PNBA_BACSU
ID PNBA_BACSU Reviewed; 489 AA.
AC P37967; O08167; O08472; P71048; Q795H9;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Para-nitrobenzyl esterase;
DE EC=3.1.1.-;
DE AltName: Full=Intracellular esterase B;
DE AltName: Full=PNB carboxy-esterase;
DE Short=PNBCE;
GN Name=pnbA; Synonyms=estB; OrderedLocusNames=BSU34390;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-22 AND
RP 211-223.
RC STRAIN=NRRL B8079;
RX PubMed=7828905; DOI=10.1016/0378-1119(94)90630-0;
RA Zock J., Cantwell C., Swartling J., Hodges R., Pohl T., Sutton K.,
RA Rosteck P. Jr., McGilvray D., Queener S.;
RT "The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning,
RT sequence and high-level expression in Escherichia coli.";
RL Gene 151:37-43(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Dartois V.A., Ferrari E., Hoch J.A.;
RT "Identification of a SinR-homolog involved in initiation of competence
RT development and sporulation in Bacillus subtilis.";
RL Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969506; DOI=10.1099/13500872-142-11-3089;
RA Fabret C., Quentin Y., Chapal N., Guiseppi A., Haiech J., Denizot F.;
RT "Integrated mapping and sequencing of a 115 kb DNA fragment from Bacillus
RT subtilis: sequence analysis of a 21 kb segment containing the sigL locus.";
RL Microbiology 142:3089-3096(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Denizot F.;
RL Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [6]
RP PROTEIN SEQUENCE OF 1-22 AND 211-223, AND CHARACTERIZATION.
RA Chen Y.-R., Usui S., Yu C.-A.;
RT "Purification and properties of p-nitrobenzyl esterase from Bacillus
RT subtilis.";
RL FASEB J. 6:A332-A332(1991).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=168;
RX PubMed=17218307; DOI=10.1074/mcp.m600464-mcp200;
RA Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R.,
RA Mann M.;
RT "The serine/threonine/tyrosine phosphoproteome of the model bacterium
RT Bacillus subtilis.";
RL Mol. Cell. Proteomics 6:697-707(2007).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
RX PubMed=10535917; DOI=10.1073/pnas.96.22.12305;
RA Spiller B., Gershenson A., Arnold F.H., Stevens R.C.;
RT "A structural view of evolutionary divergence.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:12305-12310(1999).
CC -!- FUNCTION: Catalyzes hydrolysis of several beta-lactam antibiotic PNB
CC esters to the corresponding free acid and PNB alcohol.
CC -!- SUBUNIT: Monomer.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
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DR EMBL; U06089; AAA81915.1; -; Genomic_DNA.
DR EMBL; U46134; AAB39889.1; -; Genomic_DNA.
DR EMBL; Z71928; CAA96487.1; -; Genomic_DNA.
DR EMBL; Z94043; CAB08021.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15444.1; -; Genomic_DNA.
DR PIR; B69680; B69680.
DR RefSeq; NP_391319.1; NC_000964.3.
DR RefSeq; WP_003243926.1; NZ_JNCM01000033.1.
DR PDB; 1C7I; X-ray; 2.00 A; A=1-489.
DR PDB; 1C7J; X-ray; 1.60 A; A=1-489.
DR PDB; 1QE3; X-ray; 1.50 A; A=1-489.
DR PDBsum; 1C7I; -.
DR PDBsum; 1C7J; -.
DR PDBsum; 1QE3; -.
DR AlphaFoldDB; P37967; -.
DR SMR; P37967; -.
DR STRING; 224308.BSU34390; -.
DR ESTHER; bacsu-pnbae; Carb_B_Bacteria.
DR MEROPS; S09.948; -.
DR iPTMnet; P37967; -.
DR jPOST; P37967; -.
DR PaxDb; P37967; -.
DR PRIDE; P37967; -.
DR EnsemblBacteria; CAB15444; CAB15444; BSU_34390.
DR GeneID; 938580; -.
DR KEGG; bsu:BSU34390; -.
DR PATRIC; fig|224308.179.peg.3725; -.
DR eggNOG; COG2272; Bacteria.
DR InParanoid; P37967; -.
DR OMA; CDHLVAP; -.
DR PhylomeDB; P37967; -.
DR BioCyc; BSUB:BSU34390-MON; -.
DR BRENDA; 3.1.1.1; 658.
DR EvolutionaryTrace; P37967; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0004104; F:cholinesterase activity; IEA:InterPro.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR InterPro; IPR000997; Cholinesterase.
DR Pfam; PF00135; COesterase; 1.
DR PRINTS; PR00878; CHOLNESTRASE.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Hydrolase; Phosphoprotein;
KW Reference proteome; Serine esterase.
FT CHAIN 1..489
FT /note="Para-nitrobenzyl esterase"
FT /id="PRO_0000070296"
FT ACT_SITE 189
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 310
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 399
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT MOD_RES 189
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17218307"
FT VARIANT 59
FT /note="S -> P (in strain: NRRL B8079)"
FT VARIANT 95
FT /note="K -> Q (in strain: NRRL B8079)"
FT VARIANT 150
FT /note="N -> D (in strain: NRRL B8079)"
FT VARIANT 230
FT /note="S -> A (in strain: NRRL B8079)"
FT VARIANT 242
FT /note="G -> S (in strain: NRRL B8079)"
FT VARIANT 246
FT /note="K -> R (in strain: NRRL B8079)"
FT VARIANT 251
FT /note="S -> A (in strain: NRRL B8079)"
FT VARIANT 291
FT /note="A -> S (in strain: NRRL B8079)"
FT VARIANT 343
FT /note="V -> A (in strain: NRRL B8079)"
FT VARIANT 390
FT /note="K -> E (in strain: NRRL B8079)"
FT VARIANT 463
FT /note="L -> V (in strain: NRRL B8079)"
FT CONFLICT 17
FT /note="T -> Q (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 5..8
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 11..14
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 16..18
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 21..28
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 49..53
FT /evidence="ECO:0007829|PDB:1QE3"
FT TURN 65..67
FT /evidence="ECO:0007829|PDB:1C7I"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:1C7J"
FT STRAND 84..90
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 96..103
FT /evidence="ECO:0007829|PDB:1QE3"
FT TURN 107..109
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 116..118
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 121..127
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 130..134
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 139..142
FT /evidence="ECO:0007829|PDB:1QE3"
FT TURN 147..149
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 157..172
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 173..176
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 178..188
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 190..198
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 202..204
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 209..215
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 223..237
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 244..248
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 252..263
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 274..276
FT /evidence="ECO:0007829|PDB:1C7I"
FT TURN 281..283
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 288..293
FT /evidence="ECO:0007829|PDB:1QE3"
FT TURN 294..299
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 302..307
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 310..313
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 324..335
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 337..343
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 344..346
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 351..362
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 364..374
FT /evidence="ECO:0007829|PDB:1QE3"
FT TURN 375..377
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 380..385
FT /evidence="ECO:0007829|PDB:1QE3"
FT TURN 399..402
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 403..407
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 412..417
FT /evidence="ECO:0007829|PDB:1C7J"
FT HELIX 423..442
FT /evidence="ECO:0007829|PDB:1QE3"
FT TURN 457..459
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 461..468
FT /evidence="ECO:0007829|PDB:1QE3"
FT STRAND 470..474
FT /evidence="ECO:0007829|PDB:1QE3"
FT HELIX 477..483
FT /evidence="ECO:0007829|PDB:1QE3"
SQ SEQUENCE 489 AA; 53986 MW; 95D1EF12F613C400 CRC64;
MTHQIVTTQY GKVKGTTENG VHKWKGIPYA KPPVGQWRFK APEPPEVWED VLDATAYGSI
CPQPSDLLSL SYTELPRQSE DCLYVNVFAP DTPSKNLPVM VWIHGGAFYL GAGSEPLYDG
SKLAAQGEVI VVTLNYRLGP FGFLHLSSFN EAYSDNLGLL DQAAALKWVR ENISAFGGDP
DNVTVFGESA GGMSIAALLA MPAAKGLFQK AIMESGASRT MTKEQAASTS AAFLQVLGIN
EGQLDKLHTV SAEDLLKAAD QLRIAEKENI FQLFFQPALD PKTLPEEPEK AIAEGAASGI
PLLIGTTRDE GYLFFTPDSD VHSQETLDAA LEYLLGKPLA EKVADLYPRS LESQIHMMTD
LLFWRPAVAY ASAQSHYAPV WMYRFDWHPK KPPYNKAFHA LELPFVFGNL DGLERMAKAE
ITDEVKQLSH TIQSAWITFA KTGNPSTEAV NWPAYHEETR ETLILDSEIT IENDPESEKR
QKLFPSKGE
