PIF7_TRYB2
ID PIF7_TRYB2 Reviewed; 992 AA.
AC Q57YG0; D6XMN8;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=ATP-dependent DNA helicase PIF7;
DE EC=3.6.4.12;
DE AltName: Full=DNA repair and recombination helicase PIF7;
DE Flags: Precursor;
GN Name=PIF7; ORFNames=Tb927.8.700;
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=19646907; DOI=10.1016/j.molcel.2009.07.004;
RA Liu B., Wang J., Yaffe N., Lindsay M.E., Zhao Z., Zick A., Shlomai J.,
RA Englund P.T.;
RT "Trypanosomes have six mitochondrial DNA helicases with one controlling
RT kinetoplast maxicircle replication.";
RL Mol. Cell 35:490-501(2009).
CC -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the
CC maintenance of mitochondrial (kinetoplast) genome stability.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix, kinetoplast
CC {ECO:0000269|PubMed:19646907}. Note=Localizes predominantly to the kDNA
CC disk.
CC -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AC159407; AAX69358.1; -; Genomic_DNA.
DR EMBL; CP000071; AAZ12841.1; -; Genomic_DNA.
DR RefSeq; XP_846907.1; XM_841814.1.
DR AlphaFoldDB; Q57YG0; -.
DR SMR; Q57YG0; -.
DR STRING; 5691.AAZ12841; -.
DR PaxDb; Q57YG0; -.
DR PRIDE; Q57YG0; -.
DR GeneID; 3659043; -.
DR KEGG; tbr:Tb927.8.700; -.
DR VEuPathDB; TriTrypDB:Tb927.8.700; -.
DR eggNOG; KOG0987; Eukaryota.
DR InParanoid; Q57YG0; -.
DR OMA; MEDAKND; -.
DR Proteomes; UP000008524; Chromosome 8.
DR GO; GO:0020023; C:kinetoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; ISM:GeneDB.
DR GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IC:GeneDB.
DR GO; GO:0051276; P:chromosome organization; ISA:GeneDB.
DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; ISA:GeneDB.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003840; DNA_helicase.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF02689; Herpes_Helicase; 1.
DR Pfam; PF05970; PIF1; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Helicase; Hydrolase; Kinetoplast; Mitochondrion; Nucleotide-binding;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..21
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 22..992
FT /note="ATP-dependent DNA helicase PIF7"
FT /id="PRO_0000423753"
FT DNA_BIND 651..670
FT /evidence="ECO:0000255"
FT REGION 20..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 237..244
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 992 AA; 108863 MW; B293A11FE489A0BA CRC64;
MWDGPLRSRQ NLRTVAKLRS SGCPLTQSAI HPTTTSPSEG ETASEILQGQ ASAGYIEEQQ
LPATSSQLEM SGDSNAASDN NVVIMTKRQR DDLSQASQAE VLSRLRTEDA QGPAENNGAQ
SDLALHSCDM EERVDSSKLN LNHVTVNKHS PPASKSAGIS QQNDDGGCGA SENVDNTTTA
ASKQRGKLLL DSSSSNCTPK QQAQQAVTQV PEWELSHEQE RIFDIVVNHR RSVFLTGGAG
TGKSHLLRAI IAALPLSTTF VTATTGLAAL NLGGTTLHSF SGCGFVDQHT STHQMVYRNV
LGRKKARANW RKCRVLVVDE VSMLDAWFFD MLEYVARHIR GCRKPFGGIQ LVLSGDFLQL
PPVNKHSPKQ ETRLCFEAKS WPRVNPLVCT LSHQFRQKDK EFFSLLNEVR VGALTAPSLG
LLSSLSVITT VSFVDEEKLK LKREVGAEAV DIITDSKGRT RRQRQDGFTI LRARRSEVDA
INTEKFGELD TEIYSYKGAH RGEGHFPSDL PSTVSVRAGC RVMLLANLDL SAGLANGSIG
TVESFVSSKL HQTANPSTKD DLQHLADHMM LPVVRFDHKG KQGPGDGGGA AAGRLVVIEP
HRWTMRQGDS DVSCSIQIPL QLAYAITIHK SQGMSLSHVN VDFAGIFEEG QAYVALSRCT
DVANLVIENF DAQRVNPNIK ALAYYRALEF VGTEHREAEK KLIDNGNKMN PWGPYDVEDF
EASDDDNGGA VKKEVVENLT YDAENISCMV EQFRQRYMPQ YIMFSTLRRR VLSNTEDAAR
VKGALLVMDT TSLLALTNMT GPTSLYQTIF TERGNMMRVP RVVKEELLFL ASTDVKEVSS
VTTPTLHSFC STCSSTPCST GFSYDFVEVV SCALSIMENA KCDFLLDEQR EGEANSLPPV
IQEWRSLSPL LMLNSSPDTG EKDAPSVIGF GERSREQHHS TLMFASFLVS RYSGNGAVYV
CTETVELAAR ALAIGLRVCS IAYLCNRARR VN
