ID   PIF6_TRYB2              Reviewed;         796 AA.
AC   Q38CE9;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=ATP-dependent DNA helicase PIF6;
DE            EC=3.6.4.12;
DE   AltName: Full=DNA repair and recombination helicase PIF6;
GN   Name=PIF6; ORFNames=Tb10.70.7240;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA   Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA   Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA   Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA   Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA   Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA   Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA   Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA   Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA   Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA   Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA   Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA   Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA   Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA   Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA   Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19646907; DOI=10.1016/j.molcel.2009.07.004;
RA   Liu B., Wang J., Yaffe N., Lindsay M.E., Zhao Z., Zick A., Shlomai J.,
RA   Englund P.T.;
RT   "Trypanosomes have six mitochondrial DNA helicases with one controlling
RT   kinetoplast maxicircle replication.";
RL   Mol. Cell 35:490-501(2009).
CC   -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the
CC       maintenance of genome stability. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19646907}.
CC   -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CM000208; EAN77521.1; -; Genomic_DNA.
DR   RefSeq; XP_822349.1; XM_817256.1.
DR   AlphaFoldDB; Q38CE9; -.
DR   SMR; Q38CE9; -.
DR   STRING; 5691.EAN77521; -.
DR   PaxDb; Q38CE9; -.
DR   GeneID; 3662392; -.
DR   KEGG; tbr:Tb10.70.7240; -.
DR   VEuPathDB; TriTrypDB:Tb927.10.910; -.
DR   eggNOG; KOG0987; Eukaryota.
DR   InParanoid; Q38CE9; -.
DR   OMA; KTEWLLH; -.
DR   Proteomes; UP000008524; Chromosome 10.
DR   GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR   GO; GO:0031981; C:nuclear lumen; IDA:GeneDB.
DR   GO; GO:0005634; C:nucleus; IDA:GeneDB.
DR   GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IC:GeneDB.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IC:GeneDB.
DR   GO; GO:0051276; P:chromosome organization; ISA:GeneDB.
DR   GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; ISA:GeneDB.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR   GO; GO:0000723; P:telomere maintenance; ISA:GeneDB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR010285; DNA_helicase_pif1-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF05970; PIF1; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..796
FT                   /note="ATP-dependent DNA helicase PIF6"
FT                   /id="PRO_0000423752"
FT   DNA_BIND        636..655
FT                   /evidence="ECO:0000255"
FT   REGION          197..230
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          692..719
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          762..796
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        692..718
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        762..790
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         255..262
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   796 AA;  88243 MW;  FEA5C0106E864DC6 CRC64;
     MVVLRPKITL TSVRGRIQVT AEDGERVGPW GGTECFLSRQ TGQGPCLVVR SSRHKRHQGT
     FFRLEGVRQV LSLYAMEGKL TVVVPHQKRL CSVFIETFAD VDALQMMAAT LQDRSRWKDI
     EKNVACRVQR ITRTNVVDTK RITNMDGGTD THLDYKQFEW GGGEDDSCAV GGVSCNPLEG
     ARGERNGDGV ESILQERPTG LPSAHSGGKL PKHMGGDELN PQLEGSTTPG RMQWTTDQIV
     ATRLVCTGSN VFITGSAGTG KTEWLLHLVR NVLPRDDRTV VTASTGMSAR LLGGCTIHSF
     AGIGRGEGGF NRVYNRVKSK PEVVRAWRQC QTLIIDEIGN ISPDTFSMID EIARSLRGAP
     EKPFGGIQVI LLGDFLQLPP VDSPKARNEW TNGNDTDTDS NPIPGKLKWC FETATWESLK
     LALVGFRKSY RQMNDPDFAL CLEDIRFGRY TRRVERILNE CSTRQIKERH GIEPTLIVAR
     RDEATEYNAE RLKMLEDVHF HRYESEDYAA IPGMNLEKEV SLQQLLELRI GAQVVLLASL
     PDAPHLSNGD QGVVVSFAEQ TRGPALPVVC FATSGGEEVL VPRVSMEVLG PEGRVIATRT
     QIPLQLSWAI TVHRAQGMTL PLVSVRLNKC FFDCGQAYVA LSRVRSREDL MLTAFDPSAI
     FADARAVAFY EKNFPAQRQS VEDTECELVP IKGKTRAKHP RSQGEKNSVD EGGNAPEEHP
     LRTDAAFTAY HDLDSQVSTD MPLVPQPPRK KRMLVEELPQ VTSSAIPNFT QESNNGDANS
     QLQHPFSQNN LMVDDD