ID   PHLC_STAAC              Reviewed;         330 AA.
AC   Q5HEI1;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Phospholipase C;
DE            EC=3.1.4.3;
DE   AltName: Full=Beta-hemolysin;
DE   AltName: Full=Beta-toxin;
DE   AltName: Full=Sphingomyelinase;
DE            Short=SMase;
DE   Flags: Precursor;
GN   Name=hlb; OrderedLocusNames=SACOL2003;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2726469; DOI=10.1093/nar/17.8.3305;
RA   Projan S.J., Kornblum J., Kreiswirth B., Moghazeh S.L., Eisner W.,
RA   Novick R.P.;
RT   "Nucleotide sequence: the beta-hemolysin gene of Staphylococcus aureus.";
RL   Nucleic Acids Res. 17:3305-3305(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: Bacterial hemolysins are exotoxins that attack blood cell
CC       membranes and cause cell rupture. Beta-hemolysin is a phospholipase C
CC       with specific activity toward sphingomyelins. Has a high specificity
CC       for sphingomyelin, hydrolyzes lysophosphatidylcholine at a much lower
CC       rate, but has no activity towards phosphatidylcholine,
CC       phosphatidylethanolamine, or phosphatidylserine (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC         sn-glycerol + H(+) + phosphocholine; Xref=Rhea:RHEA:10604,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815,
CC         ChEBI:CHEBI:57643, ChEBI:CHEBI:295975; EC=3.1.4.3;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neutral sphingomyelinase family.
CC       {ECO:0000305}.
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DR   EMBL; X13404; CAA31769.1; -; Genomic_DNA.
DR   EMBL; CP000046; AAW36972.1; -; Genomic_DNA.
DR   PIR; S15324; S15324.
DR   RefSeq; WP_000239607.1; NC_002951.2.
DR   AlphaFoldDB; Q5HEI1; -.
DR   SMR; Q5HEI1; -.
DR   EnsemblBacteria; AAW36972; AAW36972; SACOL2003.
DR   KEGG; sac:SACOL2003; -.
DR   HOGENOM; CLU_033415_0_0_9; -.
DR   OMA; WGQDHRA; -.
DR   PRO; PR:Q5HEI1; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0034480; F:phosphatidylcholine phospholipase C activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   CDD; cd09078; nSMase; 1.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR017766; Sphingomyelinase/PLipase_C.
DR   Pfam; PF03372; Exo_endo_phos; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
DR   TIGRFAMs; TIGR03395; sphingomy; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Disulfide bond; Hemolysis; Hydrolase; Signal; Toxin; Virulence.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000250"
FT   CHAIN           35..330
FT                   /note="Phospholipase C"
FT                   /id="PRO_0000019902"
FT   DISULFID        155..191
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   330 AA;  37238 MW;  9F4148E23091020D CRC64;
     MVKKTKSNSL KKVATLALAN LLLVGALTDN SAKAESKKDD TDLKLVSHNV YMLSTVLYPN
     WGQYKRADLI GQSSYIKNND VVIFNEAFDN GASDKLLSNV KKEYPYQTPV LGRSQSGWDK
     TEGSYSSTVA EDGGVAIVSK YPIKEKIQHV FKSGCGFDND SNKGFVYTKI EKNGKNVHVI
     GTHTQSEDSR CGAGHDRKIR AEQMKEISDF VKKKNIPKDE TVYIGGDLNV NKGTPEFKDM
     LKNLNVNDVL YAGHNSTWDP QSNSIAKYNY PNGKPEHLDY IFTDKDHKQP KQLVNEVVTE
     KPKPWDVYAF PYYYVYNDFS DHYPIKAYSK