PFL4_SCHPO
ID PFL4_SCHPO Reviewed; 609 AA.
AC Q7Z9I1;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Putative cell agglutination protein pfl4 {ECO:0000305};
DE AltName: Full=Adhesin pfl4 {ECO:0000303|PubMed:17870620};
DE AltName: Full=Pombe flocculin 4 {ECO:0000303|PubMed:23236291};
DE Flags: Precursor;
GN Name=pfl4 {ECO:0000303|PubMed:23236291};
GN ORFNames=SPCC188.09c {ECO:0000312|PomBase:SPCC188.09c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP DOMAIN, AND REPEATS.
RX PubMed=17870620; DOI=10.1016/j.fgb.2007.08.002;
RA Linder T., Gustafsson C.M.;
RT "Molecular phylogenetics of ascomycotal adhesins--a novel family of
RT putative cell-surface adhesive proteins in fission yeasts.";
RL Fungal Genet. Biol. 45:485-497(2008).
RN [3]
RP FUNCTION.
RX PubMed=23236291; DOI=10.1371/journal.pgen.1003104;
RA Kwon E.J., Laderoute A., Chatfield-Reed K., Vachon L., Karagiannis J.,
RA Chua G.;
RT "Deciphering the transcriptional-regulatory network of flocculation in
RT Schizosaccharomyces pombe.";
RL PLoS Genet. 8:E1003104-E1003104(2012).
CC -!- FUNCTION: May be involved in agglutination during conjugation or other
CC aspects of colony formation (By similarity). Induces flocculation when
CC overexpressed (PubMed:23236291). {ECO:0000250|UniProtKB:O74346,
CC ECO:0000269|PubMed:23236291}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000305|PubMed:17870620}.
CC -!- SIMILARITY: Belongs to the mam3/map4 family. {ECO:0000305}.
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DR EMBL; CU329672; CAB41229.1; -; Genomic_DNA.
DR PIR; T39124; S62518.
DR RefSeq; NP_588212.1; NM_001023202.2.
DR AlphaFoldDB; Q7Z9I1; -.
DR SMR; Q7Z9I1; -.
DR BioGRID; 275772; 38.
DR STRING; 4896.SPCC188.09c.1; -.
DR PaxDb; Q7Z9I1; -.
DR EnsemblFungi; SPCC188.09c.1; SPCC188.09c.1:pep; SPCC188.09c.
DR GeneID; 2539201; -.
DR KEGG; spo:SPCC188.09c; -.
DR PomBase; SPCC188.09c; pfl4.
DR VEuPathDB; FungiDB:SPCC188.09c; -.
DR HOGENOM; CLU_532131_0_0_1; -.
DR InParanoid; Q7Z9I1; -.
DR OMA; RPRNIYR; -.
DR PhylomeDB; Q7Z9I1; -.
DR PRO; PR:Q7Z9I1; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0010339; C:external side of cell wall; TAS:PomBase.
DR GO; GO:0000128; P:flocculation; IMP:PomBase.
DR InterPro; IPR018871; GLEYA_adhesin_domain.
DR InterPro; IPR037524; PA14/GLEYA.
DR Pfam; PF10528; GLEYA; 1.
DR PROSITE; PS51820; PA14; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Reference proteome; Repeat; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..609
FT /note="Putative cell agglutination protein pfl4"
FT /id="PRO_0000353812"
FT REPEAT 77..110
FT /note="1"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 111..146
FT /note="2"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 147..182
FT /note="3"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 183..218
FT /note="4"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 219..254
FT /note="5"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 255..290
FT /note="6"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 291..325
FT /note="7"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 326..361
FT /note="8"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 362..391
FT /note="9"
FT /evidence="ECO:0000305|PubMed:17870620"
FT DOMAIN 427..589
FT /note="PA14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01164"
FT REGION 75..391
FT /note="9 X 36 AA approximate tandem repeats"
FT /evidence="ECO:0000305|PubMed:17870620"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 609 AA; 62878 MW; 10B83A455E0362BC CRC64;
MLFLRFFIFT FFTSIFTVVV SEPPVSAKFA KRVLSATSSS SSTCPEFITI FSEGPSEYIT
TIYPSSKSSG RGTTNHSTIY TTINSGTVAS TYTVTLADGD VVVKDIEPTA GTVTTTITSG
SHEFTTTLAE ASGTVPGTVE VVEPLAGTVT TTIHSGSVEY NTTLATASGT VPGTVEVVEP
AVGTVTTTIY SGSEEFTTTL ASASGSISGT VEVIEPTAGT VTTTIYSGDQ EYTTILAEAS
GTVPGTVEVI EPAVGTVTTT TYSGSVEYTT TLVPASGSVS GTVEVVEPAV GTVTTTLQSG
SQAFTTTVPA SGSVSGTVEV VQPTGGTVTN TVYEGSQTIT STLATASGTV PGTVEVILPG
PSTIYSGTVA TTITYDVSST PASTVVVIPT AVCNGERGLQ YAVYNYDISS SKNQFCATSG
VTDVSSFTQP AYFGSSDLDQ SSPLFTGVLS SSDSVPQWTS SYNLPGYPPD ATAMGSTSSA
CKVIVYQFFF RVPVTDTFSL DVTNVDDVFY GWFGDKAISG WSNTNYDTYA YWHATNGQTG
IASFSMGSLT ADTYVPVRFV VANGAGKGGF DFSFVDSEGN SYNPTSYAYT ATCTESFLPF
GQGNGGVDN
