PESC_CANAL
ID PESC_CANAL Reviewed; 587 AA.
AC Q59X38; A0A1D8PID8;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Pescadillo homolog {ECO:0000255|HAMAP-Rule:MF_03028};
DE AltName: Full=Nucleolar protein 7 homolog {ECO:0000255|HAMAP-Rule:MF_03028};
GN Name=NOP7 {ECO:0000255|HAMAP-Rule:MF_03028};
GN OrderedLocusNames=CAALFM_C209320CA; ORFNames=CaO19.11574, CaO19.4093;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF TRP-416.
RX PubMed=19075239; DOI=10.1073/pnas.0809147105;
RA Shen J., Cowen L.E., Griffin A.M., Chan L., Koehler J.R.;
RT "The Candida albicans pescadillo homolog is required for normal hypha-to-
RT yeast morphogenesis and yeast proliferation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:20918-20923(2008).
CC -!- FUNCTION: Component of the NOP7 complex, which is required for
CC maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S
CC ribosome (By similarity). Required for the transition from hyphal to
CC yeast growth. {ECO:0000255|HAMAP-Rule:MF_03028,
CC ECO:0000269|PubMed:19075239}.
CC -!- SUBUNIT: Component of the NOP7 complex, composed of ERB1, NOP7 and
CC YTM1. The complex is held together by ERB1, which interacts with NOP7
CC via its N-terminal domain and with YTM1 via a high-affinity interaction
CC between the seven-bladed beta-propeller domains of the 2 proteins. The
CC NOP7 complex associates with the 66S pre-ribosome. {ECO:0000255|HAMAP-
CC Rule:MF_03028}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000255|HAMAP-
CC Rule:MF_03028}. Nucleus, nucleoplasm {ECO:0000255|HAMAP-Rule:MF_03028}.
CC -!- DEVELOPMENTAL STAGE: Expressed under both yeast and hyphal conditions
CC of growth. {ECO:0000269|PubMed:19075239}.
CC -!- SIMILARITY: Belongs to the pescadillo family. {ECO:0000255|HAMAP-
CC Rule:MF_03028}.
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DR EMBL; CP017624; AOW27915.1; -; Genomic_DNA.
DR RefSeq; XP_714264.2; XM_709171.2.
DR AlphaFoldDB; Q59X38; -.
DR SMR; Q59X38; -.
DR BioGRID; 1227199; 2.
DR STRING; 237561.Q59X38; -.
DR PRIDE; Q59X38; -.
DR GeneID; 3644105; -.
DR KEGG; cal:CAALFM_C209320CA; -.
DR CGD; CAL0000200824; PES1.
DR VEuPathDB; FungiDB:C2_09320C_A; -.
DR eggNOG; KOG2481; Eukaryota.
DR HOGENOM; CLU_019619_1_1_1; -.
DR InParanoid; Q59X38; -.
DR OrthoDB; 777920at2759; -.
DR PHI-base; PHI:2823; -.
DR PRO; PR:Q59X38; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070545; C:PeBoW complex; IBA:GO_Central.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IEA:UniProtKB-UniRule.
DR GO; GO:0043021; F:ribonucleoprotein complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0009267; P:cellular response to starvation; IMP:CGD.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:UniProtKB-UniRule.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0060258; P:negative regulation of filamentous growth; IMP:CGD.
DR Gene3D; 3.40.50.10190; -; 1.
DR HAMAP; MF_03028; Pescadillo; 1.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR010613; PES.
DR PANTHER; PTHR12221; PTHR12221; 1.
DR Pfam; PF16589; BRCT_2; 1.
DR Pfam; PF06732; Pescadillo_N; 1.
DR SMART; SM00292; BRCT; 1.
DR SUPFAM; SSF52113; SSF52113; 1.
DR PROSITE; PS50172; BRCT; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Ribosome biogenesis;
KW rRNA processing.
FT CHAIN 1..587
FT /note="Pescadillo homolog"
FT /id="PRO_0000370484"
FT DOMAIN 335..434
FT /note="BRCT"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03028"
FT REGION 290..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 267..306
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03028"
FT COILED 470..587
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03028"
FT COMPBIAS 459..497
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 498..545
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..570
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..587
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 416
FT /note="W->R: Temperature sensitive; impairs the transition
FT from hyphal to yeast growth on rich yeast-inducing media."
FT /evidence="ECO:0000269|PubMed:19075239"
SQ SEQUENCE 587 AA; 67891 MW; ED980B77597B50D6 CRC64;
MARIKKRGTT GNAKNFITRT QAIKKLQISL ADFRRLCIFK GIYPREPRNK KKANKGSTAP
VTFYYSKDIQ YLLHEPVLDK FRQHKTFAKK LQKALGRGEV SDAYKLDQHR PKYTLNHIIK
ERYPTFADAL RDLDDPLNML FLFANMPATD KVSAKVVSEA EKLCNQWLAY VAKERCLKKV
FVSIKGVYYQ ATVKGQEIRW LIPYKFPTNI PTDVDFRIML TFLEFYSTLV HFVLYKLYNE
AGLIYPPIIE KSIGLSGYVL QDKDAPLKKK EEKNDEEGKN LSKKELNKAI KADQEQQEND
EQDNNNGESV EDIELDEFTS TKEDSLLQPS KYASSTAELF SKFIFYIGRE VPLDILEFCI
LSCGGKIISE IAIDDLKIND PEAYKKLNLS NITHQIIDRP KILQKVPGRT YVQPQWVFDS
INKQELINVN EYAAGETLPP HLSPWGDAGG YDPNKEVEKE DGEAEEDTDE EEEEVEIEDG
DEDQEDEEEE EDEDLKAQKE LELEAAGVKF SEINEEDKKS HSKKSKGTSN KEADEEKELK
KIMMSNKQKK LFKKMQYGIE KKENREKQLT KKKKQLNKKK EQLKKLN
