PES2_ARATH
ID PES2_ARATH Reviewed; 701 AA.
AC Q9LW26;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Phytyl ester synthase 2, chloroplastic {ECO:0000303|PubMed:22623494};
DE EC=2.3.1.- {ECO:0000269|PubMed:22623494};
DE Flags: Precursor;
GN Name=PES2 {ECO:0000303|PubMed:22623494};
GN OrderedLocusNames=At3g26840 {ECO:0000312|Araport:AT3G26840};
GN ORFNames=MDJ14.16 {ECO:0000312|EMBL:BAB01231.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=16461379; DOI=10.1104/pp.105.076083;
RA Ytterberg A.J., Peltier J.-B., van Wijk K.J.;
RT "Protein profiling of plastoglobules in chloroplasts and chromoplasts. A
RT surprising site for differential accumulation of metabolic enzymes.";
RL Plant Physiol. 140:984-997(2006).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INDUCTION BY NITROGEN
RP DEPRIVATION, SUBCELLULAR LOCATION, AND CATALYTIC ACTIVITY.
RX PubMed=22623494; DOI=10.1105/tpc.112.095588;
RA Lippold F., vom Dorp K., Abraham M., Hoelzl G., Wewer V., Yilmaz J.L.,
RA Lager I., Montandon C., Besagni C., Kessler F., Stymne S., Doermann P.;
RT "Fatty acid phytyl ester synthesis in chloroplasts of Arabidopsis.";
RL Plant Cell 24:2001-2014(2012).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=22274653; DOI=10.1104/pp.111.193144;
RA Lundquist P.K., Poliakov A., Bhuiyan N.H., Zybailov B., Sun Q.,
RA van Wijk K.J.;
RT "The functional network of the Arabidopsis plastoglobule proteome based on
RT quantitative proteomics and genome-wide coexpression analysis.";
RL Plant Physiol. 158:1172-1192(2012).
CC -!- FUNCTION: Acyltransferase involved in fatty acid phytyl ester synthesis
CC in chloroplasts, a process required for the maintenance of the
CC photosynthetic membrane integrity during abiotic stress and senescence
CC (PubMed:22623494). Exhibits phytyl ester synthesis and diacylglycerol
CC acyltransferase activities with broad substrate specificities, and can
CC employ acyl-CoAs, acyl carrier proteins, and galactolipids as acyl
CC donors (PubMed:22623494). {ECO:0000269|PubMed:22623494}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + a 1,2-
CC diacylglycerol = a triacylglycerol + an acyl-3-O-(beta-D-galactosyl)-
CC sn-glycerol; Xref=Rhea:RHEA:68116, ChEBI:CHEBI:17615,
CC ChEBI:CHEBI:17855, ChEBI:CHEBI:49172, ChEBI:CHEBI:141434;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68117;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacylglycerol + a fatty acyl-CoA = a triacylglycerol +
CC CoA; Xref=Rhea:RHEA:68120, ChEBI:CHEBI:17855, ChEBI:CHEBI:49172,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68121;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacylglycerol + a fatty acyl-[ACP] = a triacylglycerol
CC + holo-[ACP]; Xref=Rhea:RHEA:68124, Rhea:RHEA-COMP:9685, Rhea:RHEA-
CC COMP:14125, ChEBI:CHEBI:17855, ChEBI:CHEBI:49172, ChEBI:CHEBI:64479,
CC ChEBI:CHEBI:138651; Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68125;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + phytol = a fatty acid phytyl ester + CoA;
CC Xref=Rhea:RHEA:68128, ChEBI:CHEBI:17327, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:77636, ChEBI:CHEBI:177021;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68129;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=phytol + tetradecanoyl-CoA = CoA + tetradecanoate phytyl
CC ester; Xref=Rhea:RHEA:68132, ChEBI:CHEBI:17327, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57385, ChEBI:CHEBI:177026;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68133;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,3-diacylglycerol + a fatty acyl-CoA = a triacylglycerol +
CC CoA; Xref=Rhea:RHEA:68152, ChEBI:CHEBI:17855, ChEBI:CHEBI:47777,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68153;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2-dihexanoylglycerol + tetradecanoyl-CoA = 1,2-dihexanoyl-3-
CC tetradecanoylglycerol + CoA; Xref=Rhea:RHEA:68196, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57385, ChEBI:CHEBI:177077, ChEBI:CHEBI:177078;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68197;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2-dihexanoylglycerol + hexadecanoyl-CoA = 1,2-dihexanoyl-3-
CC hexadecanoylglycerol + CoA; Xref=Rhea:RHEA:68200, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57379, ChEBI:CHEBI:177077, ChEBI:CHEBI:177079;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68201;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2-dihexanoylglycerol + octadecanoyl-CoA = 1,2-dihexanoyl-3-
CC octadecanoylglycerol + CoA; Xref=Rhea:RHEA:68204, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57394, ChEBI:CHEBI:177077, ChEBI:CHEBI:177080;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68205;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(7Z,10Z,13Z)-hexadecatrienoyl-CoA + 1,2-dihexanoylglycerol =
CC 1,2-dihexanoyl-3-(7Z,10Z,13Z-hexadecatrienoyl)-glycerol + CoA;
CC Xref=Rhea:RHEA:68208, ChEBI:CHEBI:57287, ChEBI:CHEBI:177077,
CC ChEBI:CHEBI:177083, ChEBI:CHEBI:177091;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68209;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1,2-dihexanoylglycerol = 1,2-
CC dihexanoyl-3-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:68212,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:177077,
CC ChEBI:CHEBI:177087; Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68213;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z,15Z)-octadecatrienoyl-CoA + 1,2-dihexanoylglycerol =
CC 1,2-dihexanoyl-3-(9Z,12Z,15Z-octadecatrienoyl)-glycerol + CoA;
CC Xref=Rhea:RHEA:68216, ChEBI:CHEBI:57287, ChEBI:CHEBI:74034,
CC ChEBI:CHEBI:177077, ChEBI:CHEBI:177088;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68217;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=decanoyl-CoA + phytol = CoA + decanoate phytyl ester;
CC Xref=Rhea:RHEA:68192, ChEBI:CHEBI:17327, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:61430, ChEBI:CHEBI:177028;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68193;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(7Z,10Z,13Z)-hexadecatrienoyl-CoA + phytol = (7Z,10Z,13Z)-
CC hexadecatrienoate phytyl ester + CoA; Xref=Rhea:RHEA:68220,
CC ChEBI:CHEBI:17327, ChEBI:CHEBI:57287, ChEBI:CHEBI:177029,
CC ChEBI:CHEBI:177091; Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68221;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dodecanoyl-CoA + phytol = CoA + dodecanoate phytyl ester;
CC Xref=Rhea:RHEA:68188, ChEBI:CHEBI:17327, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57375, ChEBI:CHEBI:177027;
CC Evidence={ECO:0000269|PubMed:22623494};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68189;
CC Evidence={ECO:0000269|PubMed:22623494};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule
CC {ECO:0000269|PubMed:16461379, ECO:0000269|PubMed:22274653,
CC ECO:0000269|PubMed:22623494}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed during senescence.
CC {ECO:0000269|PubMed:22623494}.
CC -!- INDUCTION: Accumulates upon nitrogen deprivation.
CC {ECO:0000269|PubMed:22623494}.
CC -!- DISRUPTION PHENOTYPE: Plants lacking both PES1 and PES2 grow normally
CC but show reduced phytyl ester and triacylglycerol accumulation.
CC {ECO:0000269|PubMed:22623494}.
CC -!- SIMILARITY: Belongs to the diacylglycerol acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB016889; BAB01231.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77221.1; -; Genomic_DNA.
DR EMBL; AY056370; AAL07256.1; -; mRNA.
DR EMBL; AF360145; AAK25855.1; -; mRNA.
DR RefSeq; NP_566801.1; NM_113596.2.
DR AlphaFoldDB; Q9LW26; -.
DR BioGRID; 7629; 1.
DR STRING; 3702.AT3G26840.1; -.
DR ESTHER; arath-Y3684; AlphaBeta_hydrolase.
DR iPTMnet; Q9LW26; -.
DR PaxDb; Q9LW26; -.
DR PRIDE; Q9LW26; -.
DR ProteomicsDB; 234638; -.
DR EnsemblPlants; AT3G26840.1; AT3G26840.1; AT3G26840.
DR GeneID; 822299; -.
DR Gramene; AT3G26840.1; AT3G26840.1; AT3G26840.
DR KEGG; ath:AT3G26840; -.
DR Araport; AT3G26840; -.
DR TAIR; locus:2088404; AT3G26840.
DR eggNOG; ENOG502QQUD; Eukaryota.
DR HOGENOM; CLU_015395_2_1_1; -.
DR InParanoid; Q9LW26; -.
DR OMA; HITDYIM; -.
DR OrthoDB; 1152269at2759; -.
DR PhylomeDB; Q9LW26; -.
DR PRO; PR:Q9LW26; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LW26; baseline and differential.
DR Genevisible; Q9LW26; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0010287; C:plastoglobule; IDA:UniProtKB.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IDA:TAIR.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IEP:UniProtKB.
DR GO; GO:0033306; P:phytol metabolic process; IDA:TAIR.
DR GO; GO:0090693; P:plant organ senescence; IEP:UniProtKB.
DR GO; GO:1904963; P:regulation of phytol biosynthetic process; IMP:UniProtKB.
DR GO; GO:0010866; P:regulation of triglyceride biosynthetic process; IMP:UniProtKB.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IGI:TAIR.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR007130; DAGAT.
DR Pfam; PF03982; DAGAT; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Chloroplast; Plastid; Reference proteome; Stress response;
KW Transferase; Transit peptide.
FT TRANSIT 1..65
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 66..701
FT /note="Phytyl ester synthase 2, chloroplastic"
FT /id="PRO_0000286540"
FT REGION 37..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..52
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 701 AA; 78609 MW; A7C84C000F837D73 CRC64;
MAVTVLPSVS GLSAVASSSN LRRLTSASNH RLTAIKSVTS TSSPPTPSSG VQRRRKNNDE
NRATVAKVVE NPYSKVEAAR PDLQKRLSDF LEEAREFVGD GGGPPRWFSP LECGAQATNS
PLLLYLPGID GTGLGLIRHH KKLGEIFDIW CLHIPVSDRT PVKDLVKLIE ETVKSENFRL
PNRPIYLVGE SIGACLALDV AARNPNIDLS LILVNPATHV NNFMVQPLSG MLNVLPDGLP
TLLEDIFDFG FKQGDPLTGM LDALSNEFSV QRMGGVGGGM LRDVLAVSAN LPTLSRMFPK
DTLLWKLEML KYAIASVNSH IYSVRAETLI LLSGRDHWLL KEEDIDRYSR TLPKCIVRKL
DDNGQFPLLE DGVDLATIIK CTCFYRRGKS HDHITDYIMP TTFELKQQVD DHRLLMDGTS
PVMLSTLEDG TVVRSLEGLP SEGPVLYVGY HMILGFELAP MVIQLMTERN IHLRGLAHPM
LFKNLQDSLV DTKMFDKYKI MGGVPVSHFN IYKLLREKAH VLLYPGGVRE ALHRKGEEYK
LFWPERSEFV RVASKFGAKI VPFGVVGEDD ICEIVLDSND QRNIPILKDL MEKATKDAGN
IREGDESELG NQECYFPGLV PKIPGRFYYY FGKPIETAGK EKELKDKEKA QELYLQVKSE
VEQCIDYLKV KRESDPYRHL LPRMLYQASH GWSSEIPTFD L
