PE2R4_MOUSE
ID PE2R4_MOUSE Reviewed; 513 AA.
AC P32240;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Prostaglandin E2 receptor EP4 subtype;
DE Short=PGE receptor EP4 subtype;
DE Short=PGE2 receptor EP4 subtype;
DE AltName: Full=Prostanoid EP4 receptor;
GN Name=Ptger4; Synonyms=Ptgerep4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BDF1;
RX PubMed=8385118; DOI=10.1016/s0021-9258(18)53022-2;
RA Honda A., Sugimoto Y., Namba T., Watabe A., Irie A., Negishi M.,
RA Narumiya S., Ichikawa A.;
RT "Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2
RT subtype.";
RL J. Biol. Chem. 268:7759-7762(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-317.
RX PubMed=8939885; DOI=10.1074/jbc.271.47.29569;
RA Arakawa T., Laneuville O., Miller C.A., Lakkides K.M., Wingerd B.A.,
RA Dewitt D.L., Smith W.L.;
RT "Prostanoid receptors of murine NIH 3T3 and RAW 264.7 cells. Structure and
RT expression of the murine prostaglandin EP4 receptor gene.";
RL J. Biol. Chem. 271:29569-29575(1996).
RN [3]
RP INTERACTION WITH FEM1A.
RX PubMed=18270204; DOI=10.1074/jbc.m709663200;
RA Minami M., Shimizu K., Okamoto Y., Folco E., Ilasaca M.L., Feinberg M.W.,
RA Aikawa M., Libby P.;
RT "Prostaglandin E receptor type 4-associated protein interacts directly with
RT NF-kappaB1 and attenuates macrophage activation.";
RL J. Biol. Chem. 283:9692-9703(2008).
CC -!- FUNCTION: Receptor for prostaglandin E2 (PGE2). The activity of this
CC receptor is mediated by G(s) proteins that stimulate adenylate cyclase.
CC Has a relaxing effect on smooth muscle. May play an important role in
CC regulating renal hemodynamics, intestinal epithelial transport, adrenal
CC aldosterone secretion, and uterine function.
CC -!- SUBUNIT: Interacts with FEM1A. {ECO:0000269|PubMed:18270204}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Abundant expression in ileum, thymus and
CC mastocytoma P-815 cells. Also observed in lung, spleen, heart and
CC uterus.
CC -!- PTM: Phosphorylation mediates agonist-mediated desensitization by
CC promoting cytoplasmic retention. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- CAUTION: Was originally designated as the EP2 subtype. {ECO:0000305}.
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DR EMBL; D13458; BAA02714.1; -; mRNA.
DR EMBL; U70365; AAC52897.1; -; Genomic_DNA.
DR CCDS; CCDS27365.1; -.
DR PIR; A46638; A46638.
DR RefSeq; NP_032991.1; NM_008965.2.
DR AlphaFoldDB; P32240; -.
DR SMR; P32240; -.
DR STRING; 10090.ENSMUSP00000048736; -.
DR BindingDB; P32240; -.
DR ChEMBL; CHEMBL2489; -.
DR DrugCentral; P32240; -.
DR GuidetoPHARMACOLOGY; 343; -.
DR GlyGen; P32240; 1 site.
DR iPTMnet; P32240; -.
DR PhosphoSitePlus; P32240; -.
DR PaxDb; P32240; -.
DR PeptideAtlas; P32240; -.
DR PRIDE; P32240; -.
DR ProteomicsDB; 287665; -.
DR Antibodypedia; 2762; 379 antibodies from 36 providers.
DR DNASU; 19219; -.
DR Ensembl; ENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942.
DR GeneID; 19219; -.
DR KEGG; mmu:19219; -.
DR UCSC; uc007vcy.3; mouse.
DR CTD; 5734; -.
DR MGI; MGI:104311; Ptger4.
DR VEuPathDB; HostDB:ENSMUSG00000039942; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244902; -.
DR InParanoid; P32240; -.
DR OMA; PTIPTVM; -.
DR OrthoDB; 972015at2759; -.
DR PhylomeDB; P32240; -.
DR TreeFam; TF324982; -.
DR Reactome; R-MMU-391908; Prostanoid ligand receptors.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR BioGRID-ORCS; 19219; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Ptger4; mouse.
DR PRO; PR:P32240; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; P32240; protein.
DR Bgee; ENSMUSG00000039942; Expressed in renal corpuscle and 193 other tissues.
DR ExpressionAtlas; P32240; baseline and differential.
DR Genevisible; P32240; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0044306; C:neuron projection terminus; ISO:MGI.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0004957; F:prostaglandin E receptor activity; ISS:UniProtKB.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0060348; P:bone development; ISS:UniProtKB.
DR GO; GO:0071260; P:cellular response to mechanical stimulus; IEP:UniProtKB.
DR GO; GO:0071380; P:cellular response to prostaglandin E stimulus; IBA:GO_Central.
DR GO; GO:0042466; P:chemokinesis; ISO:MGI.
DR GO; GO:0097070; P:ductus arteriosus closure; ISO:MGI.
DR GO; GO:0070371; P:ERK1 and ERK2 cascade; ISS:UniProtKB.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0007254; P:JNK cascade; ISS:UniProtKB.
DR GO; GO:0042322; P:negative regulation of circadian sleep/wake cycle, REM sleep; ISO:MGI.
DR GO; GO:0001818; P:negative regulation of cytokine production; IMP:UniProtKB.
DR GO; GO:1904336; P:negative regulation of ductus arteriosus closure; ISO:MGI.
DR GO; GO:1904471; P:negative regulation of endothelin production; ISO:MGI.
DR GO; GO:2000420; P:negative regulation of eosinophil extravasation; ISS:UniProtKB.
DR GO; GO:0010727; P:negative regulation of hydrogen peroxide metabolic process; ISO:MGI.
DR GO; GO:0050728; P:negative regulation of inflammatory response; IMP:MGI.
DR GO; GO:0033624; P:negative regulation of integrin activation; ISS:UniProtKB.
DR GO; GO:0032690; P:negative regulation of interleukin-1 alpha production; ISO:MGI.
DR GO; GO:0051771; P:negative regulation of nitric-oxide synthase biosynthetic process; ISO:MGI.
DR GO; GO:0002792; P:negative regulation of peptide secretion; ISO:MGI.
DR GO; GO:1904348; P:negative regulation of small intestine smooth muscle contraction; ISO:MGI.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; ISO:MGI.
DR GO; GO:2000388; P:positive regulation of antral ovarian follicle growth; ISO:MGI.
DR GO; GO:0045780; P:positive regulation of bone resorption; ISO:MGI.
DR GO; GO:1904364; P:positive regulation of calcitonin secretion; ISO:MGI.
DR GO; GO:0043950; P:positive regulation of cAMP-mediated signaling; ISO:MGI.
DR GO; GO:0045785; P:positive regulation of cell adhesion; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:1904367; P:positive regulation of chemokinesis; ISO:MGI.
DR GO; GO:0046010; P:positive regulation of circadian sleep/wake cycle, non-REM sleep; ISO:MGI.
DR GO; GO:0001819; P:positive regulation of cytokine production; IDA:UniProtKB.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:1900127; P:positive regulation of hyaluronan biosynthetic process; ISO:MGI.
DR GO; GO:0050729; P:positive regulation of inflammatory response; IDA:UniProtKB.
DR GO; GO:0032733; P:positive regulation of interleukin-10 production; ISO:MGI.
DR GO; GO:0032757; P:positive regulation of interleukin-8 production; ISO:MGI.
DR GO; GO:1904466; P:positive regulation of matrix metallopeptidase secretion; ISO:MGI.
DR GO; GO:0070257; P:positive regulation of mucus secretion; ISO:MGI.
DR GO; GO:2000391; P:positive regulation of neutrophil extravasation; ISO:MGI.
DR GO; GO:0045778; P:positive regulation of ossification; ISO:MGI.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; ISO:MGI.
DR GO; GO:2000386; P:positive regulation of ovarian follicle development; ISO:MGI.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:MGI.
DR GO; GO:0050714; P:positive regulation of protein secretion; ISO:MGI.
DR GO; GO:0035815; P:positive regulation of renal sodium excretion; ISO:MGI.
DR GO; GO:0014911; P:positive regulation of smooth muscle cell migration; ISO:MGI.
DR GO; GO:1904460; P:positive regulation of substance P secretion; ISO:MGI.
DR GO; GO:1904496; P:positive regulation of substance P secretion, neurotransmission; ISO:MGI.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:MGI.
DR GO; GO:0035810; P:positive regulation of urine volume; ISO:MGI.
DR GO; GO:0090303; P:positive regulation of wound healing; ISO:MGI.
DR GO; GO:0010840; P:regulation of circadian sleep/wake cycle, wakefulness; ISO:MGI.
DR GO; GO:0030278; P:regulation of ossification; IMP:MGI.
DR GO; GO:0051492; P:regulation of stress fiber assembly; IDA:UniProtKB.
DR GO; GO:0032496; P:response to lipopolysaccharide; IMP:MGI.
DR GO; GO:0009624; P:response to nematode; IMP:MGI.
DR GO; GO:0042093; P:T-helper cell differentiation; IDA:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001758; Prost_EP4_rcpt.
DR InterPro; IPR008365; Prostanoid_rcpt.
DR InterPro; IPR001244; Prostglndn_DP_rcpt.
DR PANTHER; PTHR11866; PTHR11866; 1.
DR PANTHER; PTHR11866:SF6; PTHR11866:SF6; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00428; PROSTAGLNDNR.
DR PRINTS; PR01788; PROSTANOIDR.
DR PRINTS; PR00586; PRSTNOIDEP4R.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..513
FT /note="Prostaglandin E2 receptor EP4 subtype"
FT /id="PRO_0000070065"
FT TOPO_DOM 1..44
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..68
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..80
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..104
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..121
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..140
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..185
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..209
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..236
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..323
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..340
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..360
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..513
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 383..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 385..403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 402
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P35408"
FT MOD_RES 405
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P35408"
FT MOD_RES 407
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P35408"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 117..195
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 513 AA; 56158 MW; E29216087A561473 CRC64;
MAEVGGTIPR SNRELQRCVL LTTTIMSIPG VNASFSSTPE RLNSPVTIPA VMFIFGVVGN
LVAIVVLCKS RKEQKETTFY TLVCGLAVTD LLGTLLVSPV TIATYMKGQW PGDQALCDYS
TFILLFFGLS GLSIICAMSI ERYLAINHAY FYSHYVDKRL AGLTLFAIYA SNVLFCALPN
MGLGRSERQY PGTWCFIDWT TNVTAYAAFS YMYAGFSSFL ILATVLCNVL VCGALLRMHR
QFMRRTSLGT EQHHAAAAAA VASVACRGHA GASPALQRLS DFRRRRSFRR IAGAEIQMVI
LLIATSLVVL ICSIPLVVRV FINQLYQPNV VKDISRNPDL QAIRIASVNP ILDPWIYILL
RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA MSGHSRSFLA RELKEISSTS
QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ ADTTSLRTLR ISETSDSSQG QDSESVLLVD
EVSGSHREEP ASKGNSLQVT FPSETLKLSE KCI
