OUIB_DROME
ID OUIB_DROME Reviewed; 312 AA.
AC Q9VHM6; C1C5A4; Q0IGS6;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Transcription factor Ouib {ECO:0000305|PubMed:26658797};
DE AltName: Full=Protein ouija board {ECO:0000303|PubMed:26658797};
GN Name=Ouib {ECO:0000303|PubMed:26658797, ECO:0000312|FlyBase:FBgn0037618};
GN ORFNames=CG11762 {ECO:0000312|FlyBase:FBgn0037618};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ACO72870.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Sandler J., Wan K., Yu C., Celniker S.;
RL Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=26658797; DOI=10.1371/journal.pgen.1005712;
RA Komura-Kawa T., Hirota K., Shimada-Niwa Y., Yamauchi R., Shimell M.,
RA Shinoda T., Fukamizu A., O'Connor M.B., Niwa R.;
RT "The Drosophila zinc finger transcription factor Ouija Board controls
RT ecdysteroid biosynthesis through specific regulation of spookier.";
RL PLoS Genet. 11:E1005712-E1005712(2015).
CC -!- FUNCTION: Transcription factor required for ecdysteroid production in
CC the prothoracic gland by activating transcription of the ecdysteroid
CC biosynthesis gene spok. Binds to the 5'-AGCTTTATTATTTAG-3' DNA sequence
CC in the spok enhancer region. {ECO:0000269|PubMed:26658797}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:26658797}.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in the prothoracic gland
CC during embryonic and larval development. {ECO:0000269|PubMed:26658797}.
CC -!- DEVELOPMENTAL STAGE: During larval development, expression is low
CC during early stages and gradually becomes higher in late stages.
CC {ECO:0000269|PubMed:26658797}.
CC -!- DISRUPTION PHENOTYPE: Larval developmental arrest due to ecdysteroid
CC deficiency. {ECO:0000269|PubMed:26658797}.
CC -!- MISCELLANEOUS: The name 'ouija board' derives from the instrument used
CC for calling ghosts and is based on the fact that mutants cannot induce
CC expression of the ecdysteroid biosynthesis protein spookier.
CC {ECO:0000303|PubMed:26658797}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABI34225.3; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF54277.2; -; Genomic_DNA.
DR EMBL; BT028844; ABI34225.3; ALT_INIT; mRNA.
DR EMBL; BT082033; ACO72870.1; -; mRNA.
DR EMBL; BT082125; ACQ57832.1; -; mRNA.
DR RefSeq; NP_649822.2; NM_141565.4.
DR AlphaFoldDB; Q9VHM6; -.
DR SMR; Q9VHM6; -.
DR IntAct; Q9VHM6; 1.
DR STRING; 7227.FBpp0081395; -.
DR PaxDb; Q9VHM6; -.
DR PRIDE; Q9VHM6; -.
DR DNASU; 41039; -.
DR EnsemblMetazoa; FBtr0081912; FBpp0081395; FBgn0037618.
DR GeneID; 41039; -.
DR KEGG; dme:Dmel_CG11762; -.
DR UCSC; CG11762-RA; d. melanogaster.
DR CTD; 41039; -.
DR FlyBase; FBgn0037618; ouib.
DR VEuPathDB; VectorBase:FBgn0037618; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00390000000546; -.
DR HOGENOM; CLU_002678_94_3_1; -.
DR InParanoid; Q9VHM6; -.
DR OMA; CCQSDLK; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9VHM6; -.
DR BioGRID-ORCS; 41039; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41039; -.
DR PRO; PR:Q9VHM6; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037618; Expressed in ring gland and 8 other tissues.
DR Genevisible; Q9VHM6; DM.
DR GO; GO:0005634; C:nucleus; IC:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0002168; P:instar larval development; IMP:FlyBase.
DR GO; GO:0045998; P:positive regulation of ecdysteroid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:FlyBase.
DR InterPro; IPR012934; Znf_AD.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF07776; zf-AD; 1.
DR Pfam; PF00096; zf-C2H2; 1.
DR SMART; SM00868; zf-AD; 2.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS51915; ZAD; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 5.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..312
FT /note="Transcription factor Ouib"
FT /evidence="ECO:0000305"
FT /id="PRO_0000436010"
FT DOMAIN 4..79
FT /note="ZAD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01263"
FT ZN_FING 167..189
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 195..217
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 223..245
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 251..273
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 279..303
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT BINDING 6
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01263"
FT BINDING 9
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01263"
FT BINDING 52
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01263"
FT BINDING 55
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01263"
FT CONFLICT 6
FT /note="C -> Y (in Ref. 3; ABI34225)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 312 AA; 36244 MW; 24922F50913564C7 CRC64;
MLNIVCRVCG RQKICEKSLN LFDLVNRKYL KHLHMISGLR LVDLDDVPGF MCLCCQAELR
SALAFRKLCI KTQTKWLTIE DDSSSGDEDT NDNSELESEK CAFSDFGKKK EGELVEETFQ
VLIEEEPMDK TLNRDAKAQL REDGIDEKCV PSQKIIKVST KLDDQIYICE LCGTHATSKP
TFQRHMRKHR GERPFGCKDC DARFLSAGEL RAHHRVHTGE QPFACRFCEK RYVSYMGRLI
HERTHTNDRP YVCEECGKKF TTAYVLKNHM VIHTGERNFR CDICDRSFQR KAHLVTHTRS
MMHLQNVKKQ KS
