ID   OSPZ2_SHIFL             Reviewed;         188 AA.
AC   Q2TH00;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 2.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Inactive cysteine S-methyltransferase OspZ {ECO:0000305};
DE   AltName: Full=Protein ORF212 {ECO:0000303|PubMed:17984206};
GN   Name=ospZ {ECO:0000303|PubMed:17984206};
GN   ORFNames=SFLP164 {ECO:0000312|EMBL:AAW64923.1};
OS   Shigella flexneri.
OG   Plasmid pSF5.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pSF5;
RX   PubMed=16704117; DOI=10.1007/s11427-006-0141-3;
RA   Xiong Z., Tang X., Yang F., Zhang X., Yang J., Chen L., Nie H., Yan Y.,
RA   Jiang Y., Wang J., Xue Y., Xu X., Zhu Y., Dong J., An L., Wang X., Jin Q.;
RT   "Comparison of the virulence plasmid genomes of two strains of Shigella
RT   which lost the ability to bind Congo red.";
RL   Sci. China, Ser. C, Life Sci. 49:141-148(2006).
RN   [2]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=17984206; DOI=10.1128/iai.00684-07;
RA   Zurawski D.V., Mumy K.L., Badea L., Prentice J.A., Hartland E.L.,
RA   McCormick B.A., Maurelli A.T.;
RT   "The NleE/OspZ family of effector proteins is required for
RT   polymorphonuclear transepithelial migration, a characteristic shared by
RT   enteropathogenic Escherichia coli and Shigella flexneri infections.";
RL   Infect. Immun. 76:369-379(2008).
RN   [3]
RP   FUNCTION.
RC   STRAIN=2104 / Serotype 2a;
RX   PubMed=20485572; DOI=10.1371/journal.ppat.1000898;
RA   Newton H.J., Pearson J.S., Badea L., Kelly M., Lucas M., Holloway G.,
RA   Wagstaff K.M., Dunstone M.A., Sloan J., Whisstock J.C., Kaper J.B.,
RA   Robins-Browne R.M., Jans D.A., Frankel G., Phillips A.D., Coulson B.S.,
RA   Hartland E.L.;
RT   "The type III effectors NleE and NleB from enteropathogenic E. coli and
RT   OspZ from Shigella block nuclear translocation of NF-kappaB p65.";
RL   PLoS Pathog. 6:e1000898-e1000898(2010).
CC   -!- FUNCTION: Inactive effector protein: in contrast to other members of
CC       the family, does not have the ability to inhibit host cell NF-kappa-B
CC       activation (PubMed:20485572). Probably lacks cysteine S-
CC       methyltransferase activity due to its inability to bind S-adenosyl-L-
CC       methionine at the C-terminus (Probable). {ECO:0000269|PubMed:20485572,
CC       ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17984206}. Host
CC       cytoplasm {ECO:0000269|PubMed:17984206}. Host nucleus
CC       {ECO:0000269|PubMed:17984206}. Note=Secreted via the type III secretion
CC       system (TTSS) (PubMed:17984206). Mainly localizes in the cytoplasm of
CC       the infected cells, and occasionaly in the host nucleus
CC       (PubMed:17984206). {ECO:0000269|PubMed:17984206}.
CC   -!- SIMILARITY: Belongs to the NleE/OspZ family. {ECO:0000305}.
CC   -!- CAUTION: Probable inactive cysteine methyltransferase. In contrast to
CC       other members of the family, has a shorter C-terminus and is unable to
CC       inhibit host cell NF-kappa-B activation (PubMed:20485572).
CC       {ECO:0000269|PubMed:20485572, ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAW64923.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY879342; AAW64923.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_010921740.1; NC_019197.1.
DR   RefSeq; YP_006960392.1; NC_019197.1.
DR   AlphaFoldDB; Q2TH00; -.
DR   SMR; Q2TH00; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR   GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host nucleus; Plasmid; Secreted; Virulence.
FT   CHAIN           1..188
FT                   /note="Inactive cysteine S-methyltransferase OspZ"
FT                   /id="PRO_0000452528"
SQ   SEQUENCE   188 AA;  22001 MW;  5E9633533A262A76 CRC64;
     MISPIKNIKN VFPINTANTE YIVRNIYPRV EHGYFNESPN IYDKKYISGI TRSMAQLKIE
     EFINEKSRRL NYMKTMYSPC PEDFQPISRA EASTPEGSWL TVISGKRPMG QFSVDSLYHP
     DLHALCELPE ISCKIFSKEN SDFLYIIVVF RNDSPQGELR ANRFIELYDI KREIMQVLRD
     ESPEIKVY