OSK3_ORYSJ
ID OSK3_ORYSJ Reviewed; 508 AA.
AC Q852Q0; Q10MY9; Q9ZNT4;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Serine/threonine protein kinase OSK3 {ECO:0000303|PubMed:9870704};
DE Short=OsK3 {ECO:0000303|PubMed:9870704};
DE EC=2.7.11.1 {ECO:0000269|PubMed:9870704};
DE AltName: Full=Serine/threonine protein kinase OSK35 {ECO:0000303|PubMed:16087344};
DE Short=OsK35 {ECO:0000303|PubMed:16087344};
GN Name=OSK3 {ECO:0000303|PubMed:9870704};
GN Synonyms=OSK35 {ECO:0000303|PubMed:16087344},
GN OSK5 {ECO:0000303|PubMed:9870704};
GN OrderedLocusNames=LOC_Os03g17980 {ECO:0000312|EMBL:ABF95385.1},
GN Os03g0289100 {ECO:0000312|EMBL:BAF11704.1};
GN ORFNames=OsJ_10438 {ECO:0000312|EMBL:EAZ26543.1},
GN OSNPB_030289100 {ECO:0000312|EMBL:BAS83658.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Nohrin 8;
RX PubMed=9870704; DOI=10.1007/s004380050908;
RA Takano M., Kajiya-Kanegae H., Funatsuki H., Kikuchi S.;
RT "Rice has two distinct classes of protein kinase genes related to SNF1 of
RT Saccharomyces cerevisiae, which are differently regulated in early seed
RT development.";
RL Mol. Gen. Genet. 260:388-394(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY,
RP AND GENE FAMILY.
RC STRAIN=cv. Nohrin 8;
RX PubMed=16087344; DOI=10.1016/j.plaphy.2005.06.004;
RA Kanegae H., Miyoshi K., Hirose T., Tsuchimoto S., Mori M., Nagato Y.,
RA Takano M.;
RT "Expressions of rice sucrose non-fermenting-1 related protein kinase 1
RT genes are differently regulated during the caryopsis development.";
RL Plant Physiol. Biochem. 43:669-679(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Ilpoombyeo; TISSUE=Immature seed;
RA Yoon U.H., Kim Y.H.;
RT "Molecular cloning of the serin/threonine protein kinase gene in rice.";
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [10]
RP INTERACTION WITH HDR1.
RX PubMed=26954091; DOI=10.1371/journal.pgen.1005927;
RA Sun X., Zhang Z., Wu J., Cui X., Feng D., Wang K., Xu M., Zhou L., Han X.,
RA Gu X., Lu T.;
RT "The Oryza sativa regulator HDR1 associates with the kinase OsK4 to control
RT photoperiodic flowering.";
RL PLoS Genet. 12:E1005927-E1005927(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000269|PubMed:9870704};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000269|PubMed:9870704};
CC -!- SUBUNIT: Interacts with HDR1. {ECO:0000269|PubMed:26954091}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q852Q1}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q852Q0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q852Q0-2; Sequence=VSP_058605, VSP_058606;
CC -!- TISSUE SPECIFICITY: Strongly expressed in immature seeds
CC (PubMed:9870704). Mostly expressed in panicles, and to a lower extent,
CC in leaf sheaths (PubMed:16087344). {ECO:0000269|PubMed:16087344,
CC ECO:0000269|PubMed:9870704}.
CC -!- DEVELOPMENTAL STAGE: Accumulates transiently in the early stages of
CC seed maturation (PubMed:9870704). Observed in maturating caryopsis 1
CC week after flowering (DAF) (PubMed:16087344).
CC {ECO:0000269|PubMed:16087344, ECO:0000269|PubMed:9870704}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; D82036; BAA36295.1; -; mRNA.
DR EMBL; D82038; BAA36297.1; -; mRNA.
DR EMBL; AB101657; BAC56590.1; -; Genomic_DNA.
DR EMBL; EU267991; ACA50513.1; -; mRNA.
DR EMBL; DP000009; ABF95385.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF95386.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11704.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83657.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83658.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ26543.1; -; Genomic_DNA.
DR EMBL; AK069206; BAG91315.1; -; mRNA.
DR RefSeq; XP_015632249.1; XM_015776763.1. [Q852Q0-1]
DR AlphaFoldDB; Q852Q0; -.
DR SMR; Q852Q0; -.
DR STRING; 4530.OS03T0289100-02; -.
DR PRIDE; Q852Q0; -.
DR EnsemblPlants; Os03t0289100-01; Os03t0289100-01; Os03g0289100. [Q852Q0-1]
DR EnsemblPlants; Os03t0289100-02; Os03t0289100-02; Os03g0289100. [Q852Q0-2]
DR GeneID; 4332495; -.
DR Gramene; Os03t0289100-01; Os03t0289100-01; Os03g0289100. [Q852Q0-1]
DR Gramene; Os03t0289100-02; Os03t0289100-02; Os03g0289100. [Q852Q0-2]
DR KEGG; osa:4332495; -.
DR eggNOG; KOG0583; Eukaryota.
DR InParanoid; Q852Q0; -.
DR OMA; TRTITYC; -.
DR OrthoDB; 1127668at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR028375; KA1/Ssp2_C.
DR InterPro; IPR001772; KA1_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR015940; UBA.
DR Pfam; PF02149; KA1; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF00627; UBA; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF103243; SSF103243; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50032; KA1; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS50030; UBA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding; Nucleus;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..508
FT /note="Serine/threonine protein kinase OSK3"
FT /id="PRO_0000438039"
FT DOMAIN 17..269
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 290..330
FT /note="UBA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT DOMAIN 459..507
FT /note="KA1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00565"
FT ACT_SITE 140
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 23..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 46
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT VAR_SEQ 1
FT /note="M -> MLTRTITYCMVSVTHRIHHPSIMNKLFSTAWILRHVLRWFKVKM
FT (in isoform 2)"
FT /id="VSP_058605"
FT VAR_SEQ 472..474
FT /note="Missing (in isoform 2)"
FT /id="VSP_058606"
SQ SEQUENCE 508 AA; 58250 MW; E935262080B39A59 CRC64;
MDGNAKGGGH SEALKNYNLG RTLGIGSFGK VKIAEHKLTG HRVAIKILNR RQMRNMEMEE
KAKREIKILR LFIHPHIIRL YEVIYTPTDI YVVMEYCKFG ELFDYIVEKG RLQEDEARRI
FQQIISGVEY CHRNMVVHRD LKPENLLLDS KYNVKLADFG LSNVMHDGHF LKTSCGSPNY
AAPEVISGKL YAGPEVDVWS CGVILYALLC GTLPFDDENI PNLFKKIKGG IYTLPSHLSA
LARDLIPRML VVDPMKRITI REIREHQWFQ IRLPRYLAVP PPDTAQQAKM IDEDTLQDVV
NLGYGKDHVC ESLRNRLQNE ATVAYYLLLD NRFRATSGYL GADYQESLER NFNRFASSES
ASSNTRHYLP GSSDPHASGL RPHYPVERKW ALGLQSRAQP REIMIEVLKA LQDLNVSWKK
NGQYNMKCRW SVGTQATDML DVNNSFVDDS IIMDNGDVNG RLPAVIKFEI QLYKTRDEKY
LLDMQRVTGP QLLFLDFCAD FLTKLRVL
