ID   ORF4B_BCHK4             Reviewed;         285 AA.
AC   A3EX97;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   23-FEB-2022, entry version 50.
DE   RecName: Full=Non-structural protein ORF4b;
DE            Short=ORF4b;
GN   Name=ORF4b;
OS   Bat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Merbecovirus.
OX   NCBI_TaxID=694007;
OH   NCBI_TaxID=258959; Tylonycteris pachypus (Lesser bamboo bat) (Vespertilio pachypus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU4-1;
RX   PubMed=17121802; DOI=10.1128/jvi.02182-06;
RA   Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L.,
RA   Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H.,
RA   Zheng B.-J., Yuen K.-Y.;
RT   "Comparative analysis of twelve genomes of three novel group 2c and group
RT   2d coronaviruses reveals unique group and subgroup features.";
RL   J. Virol. 81:1574-1585(2007).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=24443473; DOI=10.1099/vir.0.062059-0;
RA   Matthews K.L., Coleman C.M., van der Meer Y., Snijder E.J., Frieman M.B.;
RT   "The ORF4b-encoded accessory proteins of Middle East respiratory syndrome
RT   coronavirus and two related bat coronaviruses localize to the nucleus and
RT   inhibit innate immune signalling.";
RL   J. Gen. Virol. 95:874-882(2014).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immunity by
CC       inhibiting the interaction between host IKBKE and MAVS. In turn, this
CC       inhibition prevents the production of host interferon beta.
CC       Additionally, may also interfere with host antiviral response within
CC       the nucleus. {ECO:0000269|PubMed:24443473}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:24443473}. Host
CC       nucleus, host nucleolus {ECO:0000250|UniProtKB:K9N643}. Host cytoplasm
CC       {ECO:0000250|UniProtKB:K9N643}. Note=Mainly localized in the host
CC       nucleus. {ECO:0000250|UniProtKB:K9N643}.
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DR   EMBL; EF065505; ABN10842.1; -; Genomic_RNA.
DR   RefSeq; YP_001039956.1; NC_009019.1.
DR   GeneID; 4835994; -.
DR   KEGG; vg:4835994; -.
DR   Proteomes; UP000006574; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR   GO; GO:0039501; P:suppression by virus of host type I interferon production; IDA:UniProtKB.
DR   GO; GO:0039724; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity; IEA:UniProtKB-KW.
DR   CDD; cd21652; ORF4b_HKU4-CoV; 1.
DR   InterPro; IPR044319; ORF4b_HKU4-CoV.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host nucleus; Host-virus interaction;
KW   Inhibition of host IKBKE by virus;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host RLR pathway by virus; Reference proteome;
KW   Viral immunoevasion.
FT   CHAIN           1..285
FT                   /note="Non-structural protein ORF4b"
FT                   /id="PRO_0000290268"
SQ   SEQUENCE   285 AA;  32580 MW;  C2742B9430F7CCCC CRC64;
     MSIVLRLLSV LKHQQNKMQL DLSVNSYKLL DYSMEWSSDS VVLPPTSSDK TVMMPAKATS
     SRKRHRKPKL QYAKRRFSPV NPNDLVLVQQ EPTHCVRLVF PLSKRWIHFD GLVYSLARLN
     VSMTVDYHVT LALIYAPEAG ECFGNFLHLC PLLKDCLLEF KKLCVLGKTL TILASEWPFF
     TDVKKNKDNL TVPKAVEWLK EHGYEIYNSQ LPLHMSLAKL HDLPQAQFAE AAGLCHYFDP
     REFALPCALE VVKIGGGKVN GRSIPLARFP INNEFKFIPY LYQCV