ID   OPGH_PSEPW              Reviewed;         857 AA.
AC   B1J2R4;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072};
GN   OrderedLocusNames=PputW619_0440;
OS   Pseudomonas putida (strain W619).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=390235;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=W619;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S.,
RA   Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Kim E., Taghavi S., Vangronsveld D., van der Lelie D.,
RA   Richardson P.;
RT   "Complete sequence of Pseudomonas putida W619.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; CP000949; ACA70945.1; -; Genomic_DNA.
DR   RefSeq; WP_012312382.1; NC_010501.1.
DR   AlphaFoldDB; B1J2R4; -.
DR   STRING; 390235.PputW619_0440; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; ACA70945; ACA70945; PputW619_0440.
DR   KEGG; ppw:PputW619_0440; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   OrthoDB; 1049243at2; -.
DR   UniPathway; UPA00637; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..857
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000136658"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        515..535
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        572..592
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        606..626
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        682..702
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   857 AA;  95930 MW;  B80AA0E4A24F4E79 CRC64;
     MSNSSARPES LGEYLAHLPL SDEQRAELAS CTSFSELHQR LAGNATATTT EAVQASVGPR
     LTVGSAAELE DAEMLGVDGS GRLCLKIAPP IKRTRVVPEP WRTNVLIRMW RRMTGRTNAP
     QPPKRELPPA RWRTVGSIRR YILLTLMIGQ TLVAGWYMKG ILPYQGWSFV DLDEVVNQPL
     WDTVVQVWPY ALQTSILILF GILFCWVSAG FWTALMGFLE LLTGRDKYRI SGSSAGSEPI
     APEARTALVM PICNEDVPRV FAGLRATFES VAASGNLDRF DFFVLSDTND TDIAVAEQQA
     WLDVCRETKG FGRIFYRRRR RRVKRKSGNL DDFCRRWGGE YKYMVVLDAD SVMSGECLSS
     LVRLMEANPD AGIIQTAPKA SGMDTLYARM QQFATRVYGP LFTAGLHFWQ LGESHYWGHN
     AIIRMKPFIE HCALAPLPGK GAFAGAILSH DFVEAALMRR AGWGVWIAYD LPGSYEELPP
     NLLDELKRDR RWCHGNLMNF RLFLVKGMHP VHRAVFLTGV MSYLSAPLWF FFLVLSTALL
     ATNTLMEPQY FIEPYQLYPL WPQWHPEKAV ALFSTTIVLL FLPKLLSIIL IWAKGAVEFG
     GRIKVTLSML MEMLFSMLLA PVRMIFHTRF VLAAFLGWAA TWNSPQRDDD STPWSEAVRR
     HGPQTLLGIA WAALVAWLNP SFLWWLAPIV GSLVLSIPVS VISSRTRLGL AAKDEKLFLI
     PEEYATPQEL LATDQYTHEN RWHALHDGFV RAVVDPRQNA LACAMATARH GQAAPIEAMR
     AERLAKAIEV GPKGLDLGTR LALLSDPVAL ARLHAQVWAE HNAAWIDVWR ASINNDPHSP
     LLPLHPANEA QPSLVGA