ID   OPGH_PSEPG              Reviewed;         857 AA.
AC   B0KM50;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072};
GN   OrderedLocusNames=PputGB1_5075;
OS   Pseudomonas putida (strain GB-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=76869;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P.;
RT   "Complete sequence of Pseudomonas putida GB-1.";
RL   Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; CP000926; ABZ00960.1; -; Genomic_DNA.
DR   RefSeq; WP_012274581.1; NC_010322.1.
DR   AlphaFoldDB; B0KM50; -.
DR   STRING; 76869.PputGB1_5075; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; ABZ00960; ABZ00960; PputGB1_5075.
DR   KEGG; ppg:PputGB1_5075; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000002157; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..857
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000084499"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        515..535
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        572..592
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        606..626
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        682..702
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   857 AA;  95858 MW;  09A26A20C0056D45 CRC64;
     MSNSNARPVS LGEYLAHLPL SDEQRAELAS CSSFSELHQR LAADPAAGPA EAVQASVGPR
     LTVGSAAELE DAEMLGVDGS GRLCLKIAPP IKRTKVVPEP WRTNVLIRMW RRMTGRPNAP
     QPPKRELPPA RWRTVGSIRR YILLALMIGQ TIVAGWYMKG ILPYQGWSFV DLDEILSQSL
     WDTVVQVWPY ALQTSILILF GILFCWVSAG FWTALMGFLE LLTGRDKYKI SGSSAGNEPI
     APEARTALVM PICNEDVPRV FAGLRATFES VAASGNLDRF DFFVLSDTND TDIAVAEQQA
     WLDVCRETKG FGRIFYRRRR RRVKRKSGNL DDFCRRWGGE YKYMVVLDAD SVMSGECLSS
     LVRLMEANPD AGIIQTGPKA SGMDTLYARM QQFATRVYGP LFTAGLHFWQ LGESHYWGHN
     AIIRMKPFIE HCALAPLPGK GAFAGAILSH DFVEAALMRR AGWGVWIAYD LPGSYEELPP
     NLLDELKRDR RWCHGNLMNF RLFLVKGMHP VHRAVFLTGV MSYLSAPLWF LFLVLSTALL
     ATNTLMEPQY FIEPYQLYPL WPQWHPEKAV ALFSTTIVLL FLPKLLSVIL IWAKGAVEFG
     GRIKVTLSML MEMLFSMLLA PVRMIFHTRF VLAAFLGWAA TWNSPQRDDD STPWSEAVRR
     HGPQTLLGIA WAALVAWLNP SFLWWLAPIV GSLVLSIPVS VISSRTRLGL AAKDEKLFLI
     PEEYATPQEL LATDQYTHEN RWHALHDGFV RAVVDPRQNA LACAMATARH GQAAPIEALR
     AERVAKAMEV GPKGLDLNTR LALLSDPVAL TRLHEQVWAE HNAAWIDVWR ASIKNDPHSP
     LLPLHPESEG QPVLAGA