OIAX_RHIEC
ID OIAX_RHIEC Reviewed; 417 AA.
AC Q2JZQ0;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=3-oxo-isoapionate-4-phosphate decarboxylase {ECO:0000303|PubMed:29867142};
DE EC=4.1.1.121 {ECO:0000269|PubMed:29867142};
GN Name=oiaX {ECO:0000303|PubMed:29867142};
GN OrderedLocusNames=RHE_PF00042 {ECO:0000312|EMBL:ABC93936.1};
OS Rhizobium etli (strain CFN 42 / ATCC 51251).
OG Plasmid p42f.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=347834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CFN 42 / ATCC 51251;
RX PubMed=16505379; DOI=10.1073/pnas.0508502103;
RA Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.,
RA Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A.,
RA Jimenez-Jacinto V., Collado-Vides J., Davila G.;
RT "The partitioned Rhizobium etli genome: genetic and metabolic redundancy in
RT seven interacting replicons.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC STRAIN=CFN 42 / ATCC 51251;
RX PubMed=29867142; DOI=10.1038/s41589-018-0067-7;
RA Carter M.S., Zhang X., Huang H., Bouvier J.T., Francisco B.S.,
RA Vetting M.W., Al-Obaidi N., Bonanno J.B., Ghosh A., Zallot R.G.,
RA Andersen H.M., Almo S.C., Gerlt J.A.;
RT "Functional assignment of multiple catabolic pathways for D-apiose.";
RL Nat. Chem. Biol. 14:696-705(2018).
CC -!- FUNCTION: Involved in catabolism of D-apiose. Catalyzes the
CC decarboxylation of 3-oxo-isoapionate 4-phosphate to L-erythrulose 1-
CC phosphate. {ECO:0000269|PubMed:29867142}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-oxoisoapionate 4-phosphate + H(+) = CO2 + L-erythrulose 1-
CC phosphate; Xref=Rhea:RHEA:57072, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:58002, ChEBI:CHEBI:141357;
CC EC=4.1.1.121; Evidence={ECO:0000269|PubMed:29867142};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:O93627};
CC -!- PATHWAY: Carbohydrate metabolism. {ECO:0000269|PubMed:29867142}.
CC -!- SIMILARITY: Belongs to the RuBisCO large chain family. {ECO:0000305}.
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DR EMBL; CP000138; ABC93936.1; -; Genomic_DNA.
DR AlphaFoldDB; Q2JZQ0; -.
DR SMR; Q2JZQ0; -.
DR EnsemblBacteria; ABC93936; ABC93936; RHE_PF00042.
DR KEGG; ret:RHE_PF00042; -.
DR HOGENOM; CLU_031450_3_0_5; -.
DR OMA; IHGHPDG; -.
DR Proteomes; UP000001936; Plasmid p42f.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0016984; F:ribulose-bisphosphate carboxylase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0015977; P:carbon fixation; IEA:InterPro.
DR Gene3D; 3.20.20.110; -; 1.
DR Gene3D; 3.30.70.150; -; 1.
DR InterPro; IPR033966; RuBisCO.
DR InterPro; IPR020878; RuBisCo_large_chain_AS.
DR InterPro; IPR000685; RuBisCO_lsu_C.
DR InterPro; IPR036376; RuBisCO_lsu_C_sf.
DR InterPro; IPR017443; RuBisCO_lsu_fd_N.
DR InterPro; IPR036422; RuBisCO_lsu_N_sf.
DR PANTHER; PTHR42704; PTHR42704; 1.
DR Pfam; PF00016; RuBisCO_large; 1.
DR Pfam; PF02788; RuBisCO_large_N; 1.
DR SFLD; SFLDS00014; RuBisCO; 1.
DR SUPFAM; SSF51649; SSF51649; 1.
DR SUPFAM; SSF54966; SSF54966; 1.
DR PROSITE; PS00157; RUBISCO_LARGE; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Decarboxylase; Lyase; Magnesium; Metal-binding;
KW Plasmid; Reference proteome.
FT CHAIN 1..417
FT /note="3-oxo-isoapionate-4-phosphate decarboxylase"
FT /id="PRO_0000450939"
FT BINDING 177
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /note="via carbamate group"
FT /evidence="ECO:0000250|UniProtKB:O93627"
FT BINDING 179
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:O93627"
FT BINDING 180
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:O93627"
FT MOD_RES 177
FT /note="N6-carboxylysine"
FT /evidence="ECO:0000250|UniProtKB:O93627"
SQ SEQUENCE 417 AA; 44833 MW; 508F61FEB05F85DF CRC64;
MITLTYRIET PGSVETMADK IASDQSTGTF VPVPGETEEL KSRVAARVLA IRPLENARHP
TWPESAPDTL LHRADVDIAF PLEAIGTDLS ALMTIAIGGV YSIKGMTGIR IVDMKLPEAF
RSAHPGPQFG IAGSRRLTGV EGRPIIGTIV KPALGLRPHE TAELVGELIG SGVDFIKDDE
KLMSPAYSPL KERVAAIMPR ILDHEQKTGK KVMYAFGISH ADPDEMMRNH DIVAAAGGNC
AVVNINSIGF GGMSFLRKRS SLVLHAHRNG WDVLTRDPGA GMDFKVYQQF WRLLGVDQFQ
INGIRIKYWE PDESFVSSFK AVSTPLFDAA DCPLPVAGSG QWGGQAPETY ERTGRTIDLL
YLCGGGIVSH PGGPAAGVRA VQQAWQAAVA GIPLEVYAKD HPELAASIAK FSDGKGA
