OHP2_ORYSJ
ID OHP2_ORYSJ Reviewed; 149 AA.
AC Q6VAK4; A0A0P0XRE0; Q0IZJ0;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Histidine-containing phosphotransfer protein 2 {ECO:0000305};
DE AltName: Full=OsAHP2 {ECO:0000303|PubMed:24578505};
DE AltName: Full=OsHP1 {ECO:0000312|EMBL:AAQ24029.1};
DE AltName: Full=OsHpt3 {ECO:0000303|PubMed:16891544};
GN Name=AHP2 {ECO:0000303|PubMed:17284581};
GN Synonyms=HP1 {ECO:0000312|EMBL:AAQ24029.1},
GN OHP2 {ECO:0000303|PubMed:16919402};
GN OrderedLocusNames=Os09g0567400 {ECO:0000312|EMBL:BAF25875.1},
GN LOC_Os09g39400 {ECO:0000305};
GN ORFNames=OJ1155_H10.30 {ECO:0000312|EMBL:BAD46253.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=16919402; DOI=10.1016/j.gene.2006.06.020;
RA Ito Y., Kurata N.;
RT "Identification and characterization of cytokinin-signalling gene families
RT in rice.";
RL Gene 382:57-65(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Gu Z.M., Zhang H.S., Huang J.;
RT "Characterization of genes for two-component phosphorelay mediators with a
RT single HPt domain in Oryza sativa.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=16891544; DOI=10.1104/pp.106.086371;
RA Pareek A., Singh A., Kumar M., Kushwaha H.R., Lynn A.M.,
RA Singla-Pareek S.L.;
RT "Whole-genome analysis of Oryza sativa reveals similar architecture of two-
RT component signaling machinery with Arabidopsis.";
RL Plant Physiol. 142:380-397(2006).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=17408920; DOI=10.1016/j.ygeno.2007.02.001;
RA Du L., Jiao F., Chu J., Jin G., Chen M., Wu P.;
RT "The two-component signal system in rice (Oryza sativa L.): a genome-wide
RT study of cytokinin signal perception and transduction.";
RL Genomics 89:697-707(2007).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17284581; DOI=10.1104/pp.106.093666;
RA Schaller G.E., Doi K., Hwang I., Kieber J.J., Khurana J.P., Kurata N.,
RA Mizuno T., Pareek A., Shiu S.H., Wu P., Yip W.K.;
RT "Nomenclature for two-component signaling elements of rice.";
RL Plant Physiol. 143:555-557(2007).
RN [9]
RP SUBCELLULAR LOCATION.
RX PubMed=22383541; DOI=10.1104/pp.111.192765;
RA Tsai Y.C., Weir N.R., Hill K., Zhang W., Kim H.J., Shiu S.H.,
RA Schaller G.E., Kieber J.J.;
RT "Characterization of genes involved in cytokinin signaling and metabolism
RT from rice.";
RL Plant Physiol. 158:1666-1684(2012).
RN [10]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24578505; DOI=10.1104/pp.113.232629;
RA Sun L., Zhang Q., Wu J., Zhang L., Jiao X., Zhang S., Zhang Z., Sun D.,
RA Lu T., Sun Y.;
RT "Two rice authentic histidine phosphotransfer proteins, OsAHP1 and OsAHP2,
RT mediate cytokinin signaling and stress responses in rice.";
RL Plant Physiol. 165:335-345(2014).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-149.
RG Center for eukaryotic structural genomics (CESG);
RT "X-ray structure of putative histidine-containing phosphotransfer protein
RT from rice, AK104879.";
RL Submitted (FEB-2005) to the PDB data bank.
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 2-149.
RX PubMed=17850744; DOI=10.1016/j.str.2007.06.019;
RA Levin E.J., Kondrashov D.A., Wesenberg G.E., Phillips G.N. Jr.;
RT "Ensemble refinement of protein crystal structures: validation and
RT application.";
RL Structure 15:1040-1052(2007).
CC -!- FUNCTION: Functions as two-component phosphorelay mediators between
CC cytokinin sensor histidine kinases and response regulators (B-type
CC ARRs). Plays an important role in propagating cytokinin signal
CC transduction through the multistep His-to-Asp phosphorelay (By
CC similarity). Functions as positive regulator of the cytokinin signaling
CC pathway. May play a regulatory role in salt and drought tolerance
CC during plant development (PubMed:24578505).
CC {ECO:0000250|UniProtKB:Q8L9T7, ECO:0000269|PubMed:24578505}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:22383541,
CC ECO:0000269|PubMed:24578505}. Nucleus {ECO:0000269|PubMed:22383541,
CC ECO:0000269|PubMed:24578505}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:16919402,
CC ECO:0000269|PubMed:17408920}.
CC -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC active histidine that mediates the phosphotransfer. {ECO:0000305}.
CC -!- PTM: Two-component system major event consists of a His-to-Asp
CC phosphorelay between a sensor histidine kinase (HK) and a response
CC regulator (RR). In plants, the His-to-Asp phosphorelay involves an
CC additional intermediate named Histidine-containing phosphotransfer
CC protein (HPt). This multistep phosphorelay consists of a His-Asp-His-
CC Asp sequential transfer of a phosphate group between first an His and
CC an Asp of the HK protein, followed by the transfer to a conserved His
CC of the HPt protein and finally the transfer to an Asp in the receiver
CC domain of the RR protein. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Dwarf, narrow leaf, small grain and low fertility
CC phenotypes. {ECO:0000269|PubMed:16891544}.
CC -!- MISCELLANEOUS: Plant silencing simultaneously OHP1 and OHP2 exhibit
CC phenotypes for a deficiency in cytokinin signaling, including dwarfism
CC with reduced internode lengths, enhanced lateral root growth, early
CC leaf senescence, and reduced tiller numbers and fertility.
CC {ECO:0000269|PubMed:24578505}.
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DR EMBL; BR000249; FAA00253.1; -; Genomic_DNA.
DR EMBL; AY345241; AAQ24029.1; -; mRNA.
DR EMBL; AP005558; BAD46253.1; -; Genomic_DNA.
DR EMBL; AP008215; BAF25875.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT09475.1; -; Genomic_DNA.
DR EMBL; CM000146; EEE70257.1; -; Genomic_DNA.
DR RefSeq; XP_015611768.1; XM_015756282.1.
DR PDB; 1YVI; X-ray; 2.00 A; A/B=2-149.
DR PDB; 2Q4F; X-ray; 2.00 A; A/B=2-149.
DR PDBsum; 1YVI; -.
DR PDBsum; 2Q4F; -.
DR AlphaFoldDB; Q6VAK4; -.
DR SMR; Q6VAK4; -.
DR STRING; 4530.OS09T0567400-01; -.
DR PaxDb; Q6VAK4; -.
DR PRIDE; Q6VAK4; -.
DR EnsemblPlants; Os09t0567400-01; Os09t0567400-01; Os09g0567400.
DR EnsemblPlants; Os09t0567400-02; Os09t0567400-02; Os09g0567400.
DR GeneID; 4347886; -.
DR Gramene; Os09t0567400-01; Os09t0567400-01; Os09g0567400.
DR Gramene; Os09t0567400-02; Os09t0567400-02; Os09g0567400.
DR KEGG; osa:4347886; -.
DR eggNOG; KOG4747; Eukaryota.
DR HOGENOM; CLU_111777_3_0_1; -.
DR InParanoid; Q6VAK4; -.
DR OMA; CATFRTF; -.
DR OrthoDB; 1488145at2759; -.
DR EvolutionaryTrace; Q6VAK4; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000007752; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR Genevisible; Q6VAK4; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009927; F:histidine phosphotransfer kinase activity; IBA:GO_Central.
DR GO; GO:0043424; F:protein histidine kinase binding; IBA:GO_Central.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IBA:GO_Central.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IBA:GO_Central.
DR GO; GO:0080038; P:positive regulation of cytokinin-activated signaling pathway; IMP:UniProtKB.
DR CDD; cd00088; HPT; 1.
DR Gene3D; 1.20.120.160; -; 1.
DR InterPro; IPR045871; AHP1-5/YPD1.
DR InterPro; IPR036641; HPT_dom_sf.
DR InterPro; IPR008207; Sig_transdc_His_kin_Hpt_dom.
DR PANTHER; PTHR28242; PTHR28242; 1.
DR Pfam; PF01627; Hpt; 1.
DR SUPFAM; SSF47226; SSF47226; 1.
DR PROSITE; PS50894; HPT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytokinin signaling pathway; Cytoplasm; Nucleus;
KW Phosphoprotein; Reference proteome; Sensory transduction;
KW Two-component regulatory system.
FT CHAIN 1..149
FT /note="Histidine-containing phosphotransfer protein 2"
FT /id="PRO_0000247615"
FT DOMAIN 39..144
FT /note="HPt"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT MOD_RES 80
FT /note="Phosphohistidine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT HELIX 2..19
FT /evidence="ECO:0007829|PDB:1YVI"
FT HELIX 25..34
FT /evidence="ECO:0007829|PDB:1YVI"
FT HELIX 42..65
FT /evidence="ECO:0007829|PDB:1YVI"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:1YVI"
FT HELIX 72..89
FT /evidence="ECO:0007829|PDB:1YVI"
FT HELIX 92..106
FT /evidence="ECO:0007829|PDB:1YVI"
FT HELIX 110..142
FT /evidence="ECO:0007829|PDB:1YVI"
SQ SEQUENCE 149 AA; 16811 MW; 38CAED8B091225D3 CRC64;
MAAAALRDQL TALLSSMFSQ GLVDEQFQQL QMLQDEGGTP GFVSEVVTLF CDDADRIINE
IATLLEQPVV NFDKVDAYVH QLKGSSASVG AQKVKFTCMQ FRQFCQDKSR DGCLMALAVV
RNDFYDLRNK FQTMLQLEQQ IQAYDPKQQ
