ARPC3_ARATH
ID ARPC3_ARATH Reviewed; 174 AA.
AC Q1ECJ7; O80764; Q8LFW2;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Actin-related protein 2/3 complex subunit 3;
DE AltName: Full=Actin-related protein C3;
DE AltName: Full=Arp2/3 complex 21 kDa subunit;
DE Short=p21-ARC;
GN Name=ARPC3; OrderedLocusNames=At1g60430; ORFNames=T13D8.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY, AND IDENTIFICATION OF THE ARP2/3 COMPLEX.
RX PubMed=12913159; DOI=10.1104/pp.103.028563;
RA Li S., Blanchoin L., Yang Z., Lord E.M.;
RT "The putative Arabidopsis arp2/3 complex controls leaf cell
RT morphogenesis.";
RL Plant Physiol. 132:2034-2044(2003).
RN [6]
RP REVIEW.
RX PubMed=15653407; DOI=10.1016/j.pbi.2004.11.004;
RA Szymanski D.B.;
RT "Breaking the WAVE complex: the point of Arabidopsis trichomes.";
RL Curr. Opin. Plant Biol. 8:103-112(2005).
RN [7]
RP INTERACTION WITH SCAR2/DIS3.
RX PubMed=15659634; DOI=10.1105/tpc.104.027987;
RA Basu D., Le J., El-Din El-Assal S., Huang S., Zhang C., Mallery E.L.,
RA Koliantz G., Staiger C.J., Szymanski D.B.;
RT "DISTORTED3/SCAR2 is a putative Arabidopsis WAVE complex subunit that
RT activates the Arp2/3 complex and is required for epidermal morphogenesis.";
RL Plant Cell 17:502-524(2005).
CC -!- FUNCTION: Functions as component of the Arp2/3 complex which is
CC involved in regulation of actin polymerization and together with an
CC activating nucleation-promoting factor (NPF) mediates the formation of
CC branched actin networks. Arp2/3 complex plays a critical role in the
CC control of cell morphogenesis via the modulation of cell polarity
CC development. {ECO:0000250}.
CC -!- SUBUNIT: Component of the Arp2/3 complex composed of ARP2, ARP3,
CC ARPC1/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and
CC ARPC5/p16-ARC. Interacts with SCAR2/DIS3 (WAVE complex).
CC {ECO:0000269|PubMed:15659634}.
CC -!- INTERACTION:
CC Q1ECJ7; Q5XPJ9: SCAR2; NbExp=4; IntAct=EBI-1547752, EBI-1547795;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Cell
CC projection {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in all tissues with a
CC relatively highest expression in inflorescences.
CC {ECO:0000269|PubMed:12913159}.
CC -!- SIMILARITY: Belongs to the ARPC3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24070.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004473; AAC24070.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33685.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33686.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59520.1; -; Genomic_DNA.
DR EMBL; BT025737; ABF83627.1; -; mRNA.
DR EMBL; AY084614; AAM61177.1; -; mRNA.
DR PIR; T02293; T02293.
DR RefSeq; NP_001077743.1; NM_001084274.2.
DR RefSeq; NP_001319276.1; NM_001333892.1.
DR RefSeq; NP_564757.1; NM_104730.3.
DR AlphaFoldDB; Q1ECJ7; -.
DR SMR; Q1ECJ7; -.
DR BioGRID; 27562; 8.
DR IntAct; Q1ECJ7; 8.
DR STRING; 3702.AT1G60430.2; -.
DR PaxDb; Q1ECJ7; -.
DR PRIDE; Q1ECJ7; -.
DR ProteomicsDB; 246937; -.
DR EnsemblPlants; AT1G60430.1; AT1G60430.1; AT1G60430.
DR EnsemblPlants; AT1G60430.2; AT1G60430.2; AT1G60430.
DR EnsemblPlants; AT1G60430.3; AT1G60430.3; AT1G60430.
DR GeneID; 842338; -.
DR Gramene; AT1G60430.1; AT1G60430.1; AT1G60430.
DR Gramene; AT1G60430.2; AT1G60430.2; AT1G60430.
DR Gramene; AT1G60430.3; AT1G60430.3; AT1G60430.
DR KEGG; ath:AT1G60430; -.
DR Araport; AT1G60430; -.
DR TAIR; locus:2195633; AT1G60430.
DR eggNOG; KOG3155; Eukaryota.
DR HOGENOM; CLU_094365_0_0_1; -.
DR InParanoid; Q1ECJ7; -.
DR OMA; TPSKWWL; -.
DR OrthoDB; 1451115at2759; -.
DR PhylomeDB; Q1ECJ7; -.
DR PRO; PR:Q1ECJ7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q1ECJ7; baseline and differential.
DR Genevisible; Q1ECJ7; AT.
DR GO; GO:0005885; C:Arp2/3 protein complex; IBA:GO_Central.
DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0007015; P:actin filament organization; TAS:TAIR.
DR GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IBA:GO_Central.
DR GO; GO:0030833; P:regulation of actin filament polymerization; IEA:InterPro.
DR Gene3D; 1.10.1760.10; -; 1.
DR InterPro; IPR007204; ARPC3.
DR InterPro; IPR036753; ARPC3_sf.
DR PANTHER; PTHR12391; PTHR12391; 1.
DR Pfam; PF04062; P21-Arc; 1.
DR PIRSF; PIRSF016315; ARP2/3_P21-Arc; 1.
DR SUPFAM; SSF69060; SSF69060; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Cell projection; Cytoplasm; Cytoskeleton;
KW Reference proteome.
FT CHAIN 1..174
FT /note="Actin-related protein 2/3 complex subunit 3"
FT /id="PRO_0000422530"
FT CONFLICT 13
FT /note="V -> I (in Ref. 4; AAM61177)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="V -> I (in Ref. 4; AAM61177)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 174 AA; 19491 MW; 9383A162D10E62CA CRC64;
MVYHSSFVDE EGVTKACGCP LLPLKSHIKG PAPVSEQDKT DIVDEAITFF RANVFFTNFD
IKSPADKLLI YLTFYINVAL KRLEGCRTLA VGTKAIINLG LEDIPVPGET GFPFPGLFSL
PQSQDEAELF RNYLKQVREE TSGRLLSVAY RANGTPNKWW LAFAKRKFMN VVVL
