ID   ARP3_ACACA              Reviewed;         427 AA.
AC   P53490;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Actin-related protein 3;
DE   AltName: Full=Actin-like protein 3;
GN   Name=ARP3;
OS   Acanthamoeba castellanii (Amoeba).
OC   Eukaryota; Amoebozoa; Discosea; Longamoebia; Centramoebida;
OC   Acanthamoebidae; Acanthamoeba.
OX   NCBI_TaxID=5755;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=ATCC 30010 / Neff;
RX   PubMed=7593166; DOI=10.1083/jcb.131.2.385;
RA   Kelleher J.F., Atkinson S.J., Pollard T.D.;
RT   "Sequences, structural models, and cellular localization of the actin-
RT   related proteins Arp2 and Arp3 from Acanthamoeba.";
RL   J. Cell Biol. 131:385-397(1995).
CC   -!- FUNCTION: Functions as ATP-binding component of the Arp2/3 complex
CC       which is involved in regulation of actin polymerization and together
CC       with an activating nucleation-promoting factor (NPF) mediates the
CC       formation of branched actin networks. Seems to contact the pointed end
CC       of the daughter actin filament (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Arp2/3 complex composed of ARP2, ARP3,
CC       ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and
CC       ARPC5/p16-ARC. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:P42528}. Cell projection
CC       {ECO:0000250|UniProtKB:P42528}.
CC   -!- SIMILARITY: Belongs to the actin family. ARP3 subfamily. {ECO:0000305}.
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DR   EMBL; U29610; AAA93068.1; -; mRNA.
DR   AlphaFoldDB; P53490; -.
DR   SMR; P53490; -.
DR   VEuPathDB; AmoebaDB:ACA1_396540; -.
DR   GO; GO:0005885; C:Arp2/3 protein complex; IEA:InterPro.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro.
DR   GO; GO:0006909; P:phagocytosis; IEA:UniProt.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   InterPro; IPR004000; Actin.
DR   InterPro; IPR020902; Actin/actin-like_CS.
DR   InterPro; IPR015623; Arp3.
DR   InterPro; IPR043129; ATPase_NBD.
DR   PANTHER; PTHR11937; PTHR11937; 1.
DR   PANTHER; PTHR11937:SF175; PTHR11937:SF175; 1.
DR   Pfam; PF00022; Actin; 1.
DR   SMART; SM00268; ACTIN; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   PROSITE; PS01132; ACTINS_ACT_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Actin-binding; ATP-binding; Cell projection; Cytoplasm; Cytoskeleton;
KW   Nucleotide-binding.
FT   CHAIN           1..427
FT                   /note="Actin-related protein 3"
FT                   /id="PRO_0000089083"
SQ   SEQUENCE   427 AA;  48636 MW;  A53A0965B1E7EAF6 CRC64;
     MSRSGLPAVV IDNGTGYTKM GYAGNTEPQY IIPTAIATKG IAEDPRCRAR RWWCPWAAGK
     NIADLDFFIG DEAYENSKVY QITMPVRHGQ VENWTHMEQF WEHCIFKYLR CEPEDHHFLL
     TEPPLNAPEN REYTAEIMFE TFNVPGLYIA VQAVLALAAS WTSKQVTEKT LTGTVIDSGD
     GVTHVIPVAE GYVIGSSIKH IPLAGRDITN FVLQLLRERN EKIPPAETLE VAKRIKETFS
     YVCPDIVKEF KKYDTEPDKW FKTYEGIESV GKKPYNVDVG YERFLGPEIF FNPEIFSSDF
     LTPLPKVVDE TIQSCPIDTR RGLYKNIVLS GGSTMFKDFG KRLQRDIKRA VDYRIKRSEE
     LSQGRIKSKA VDVKVISHHM QRFAVWFGGS MLASTPEFYK VCHTKQQYDE VGPSICRHNP
     VFGAMTM