ID   ARP3B_XENLA             Reviewed;         418 AA.
AC   A0A1L8EV45;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=Actin-related protein 3-B {ECO:0000305};
GN   Name=actr3-b; ORFNames=XELAEV_18044311mg {ECO:0000312|EMBL:OCT63212.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J;
RX   PubMed=27762356; DOI=10.1038/nature19840;
RA   Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA   Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA   Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA   Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA   Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA   Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA   Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA   Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA   Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA   Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA   Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA   Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA   Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT   "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL   Nature 538:336-343(2016).
CC   -!- FUNCTION: ATP-binding component of the Arp2/3 complex, a multiprotein
CC       complex that mediates actin polymerization upon stimulation by
CC       nucleation-promoting factor (NPF). The Arp2/3 complex mediates the
CC       formation of branched actin networks in the cytoplasm, providing the
CC       force for cell motility (By similarity). Seems to contact the pointed
CC       end of the daughter actin filament (By similarity). In addition to its
CC       role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes
CC       actin polymerization in the nucleus, thereby regulating gene
CC       transcription and repair of damaged DNA (By similarity). The Arp2/3
CC       complex promotes homologous recombination (HR) repair in response to
CC       DNA damage by promoting nuclear actin polymerization, leading to drive
CC       motility of double-strand breaks (DSBs) (By similarity).
CC       {ECO:0000250|UniProtKB:P61158, ECO:0000250|UniProtKB:Q801P7}.
CC   -!- SUBUNIT: Component of the Arp2/3 complex composed of actr2/arp2,
CC       actr3/arp3, arpc1 (arpc1a or arpc1b), arpc2, arpc3, arpc4 and arpc5.
CC       {ECO:0000250|UniProtKB:Q801P7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q801P7}. Cell projection
CC       {ECO:0000250|UniProtKB:Q801P7}. Nucleus {ECO:0000250|UniProtKB:P61158}.
CC   -!- SIMILARITY: Belongs to the actin family. ARP3 subfamily. {ECO:0000305}.
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DR   EMBL; CM004482; OCT63212.1; -; Genomic_DNA.
DR   RefSeq; XP_018091075.1; XM_018235586.1.
DR   AlphaFoldDB; A0A1L8EV45; -.
DR   SMR; A0A1L8EV45; -.
DR   STRING; 8355.A0A1L8EV45; -.
DR   GeneID; 108701232; -.
DR   KEGG; xla:108701232; -.
DR   CTD; 108701232; -.
DR   Xenbase; XB-GENE-17335395; actr3.L.
DR   OMA; TAEFKSY; -.
DR   OrthoDB; 649708at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   Bgee; 108701232; Expressed in spleen and 19 other tissues.
DR   GO; GO:0005885; C:Arp2/3 protein complex; ISS:UniProtKB.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0035861; C:site of double-strand break; ISS:UniProtKB.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro.
DR   InterPro; IPR004000; Actin.
DR   InterPro; IPR020902; Actin/actin-like_CS.
DR   InterPro; IPR015623; Arp3.
DR   InterPro; IPR043129; ATPase_NBD.
DR   PANTHER; PTHR11937; PTHR11937; 1.
DR   PANTHER; PTHR11937:SF175; PTHR11937:SF175; 1.
DR   Pfam; PF00022; Actin; 1.
DR   SMART; SM00268; ACTIN; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   PROSITE; PS01132; ACTINS_ACT_LIKE; 1.
PE   3: Inferred from homology;
KW   Actin-binding; ATP-binding; Cell projection; Cytoplasm; Cytoskeleton;
KW   Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..418
FT                   /note="Actin-related protein 3-B"
FT                   /id="PRO_0000445572"
SQ   SEQUENCE   418 AA;  47262 MW;  DB7EC96750AEA3CE CRC64;
     MAARLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVGDQAQRR LMKGVDDLDF
     HIGDEAIDKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY LRAEPEDHYF LLTEPPLNTP
     ENREYTAEIM FESFNVPGLY IAVQAVLALA ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV
     AEGYVIGSCI KHIPIAGRDI TYFTQQLLRD REVGIPPEQS LETAKAVKER FSYVCPDLVK
     EFSKYDTDGA KWIKQYTGVN AVSKKEFSID VGYERFLGPE IFFHPEFANP DFTQPISEVV
     DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDVK RTVDARLKLS EELSGGRLKP
     KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY EEIGPSICRH NPVFGVMS