NUD24_ARATH
ID NUD24_ARATH Reviewed; 365 AA.
AC P0C026;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Nudix hydrolase 24, chloroplastic;
DE Short=AtNUDT24;
DE EC=3.6.1.-;
DE Flags: Precursor;
GN Name=NUDT24; Synonyms=NUDX24; OrderedLocusNames=At5g19470;
GN ORFNames=F7K24.220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NOMENCLATURE.
RX PubMed=15878881; DOI=10.1074/jbc.m503536200;
RA Ogawa T., Ueda Y., Yoshimura K., Shigeoka S.;
RT "Comprehensive analysis of cytosolic nudix hydrolases in Arabidopsis
RT thaliana.";
RL J. Biol. Chem. 280:25277-25283(2005).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=18815383; DOI=10.1104/pp.108.128413;
RA Ogawa T., Yoshimura K., Miyake H., Ishikawa K., Ito D., Tanabe N.,
RA Shigeoka S.;
RT "Molecular characterization of organelle-type Nudix hydrolases in
RT Arabidopsis.";
RL Plant Physiol. 148:1412-1424(2008).
CC -!- FUNCTION: Probably mediates the hydrolysis of some nucleoside
CC diphosphate derivatives. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P0C026-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:18815383}.
CC -!- SIMILARITY: Belongs to the Nudix hydrolase family. {ECO:0000305}.
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DR EMBL; AF296837; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92711.1; -; Genomic_DNA.
DR RefSeq; NP_197448.2; NM_121952.3. [P0C026-1]
DR AlphaFoldDB; P0C026; -.
DR SMR; P0C026; -.
DR STRING; 3702.AT5G19470.1; -.
DR PaxDb; P0C026; -.
DR PRIDE; P0C026; -.
DR ProteomicsDB; 234940; -. [P0C026-1]
DR EnsemblPlants; AT5G19470.1; AT5G19470.1; AT5G19470. [P0C026-1]
DR GeneID; 832067; -.
DR Gramene; AT5G19470.1; AT5G19470.1; AT5G19470. [P0C026-1]
DR KEGG; ath:AT5G19470; -.
DR Araport; AT5G19470; -.
DR TAIR; locus:2150260; AT5G19470.
DR eggNOG; KOG4313; Eukaryota.
DR InParanoid; P0C026; -.
DR OMA; ETFPVYG; -.
DR PhylomeDB; P0C026; -.
DR PRO; PR:P0C026; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P0C026; baseline and differential.
DR Genevisible; P0C026; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0044715; F:8-oxo-dGDP phosphatase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR031804; DUF4743.
DR InterPro; IPR015797; NUDIX_hydrolase-like_dom_sf.
DR InterPro; IPR000086; NUDIX_hydrolase_dom.
DR Pfam; PF15916; DUF4743; 1.
DR Pfam; PF00293; NUDIX; 1.
DR SUPFAM; SSF55811; SSF55811; 1.
DR PROSITE; PS51462; NUDIX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Hydrolase; Magnesium; Manganese;
KW Metal-binding; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 31..365
FT /note="Nudix hydrolase 24, chloroplastic"
FT /id="PRO_0000019966"
FT DOMAIN 196..337
FT /note="Nudix hydrolase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00794"
FT MOTIF 235..256
FT /note="Nudix box"
FT BINDING 250
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 254
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
SQ SEQUENCE 365 AA; 41056 MW; 7E84C2B840CB3919 CRC64;
MASAFCSLCP TPTSLFSSHA LIPTLQWRSS SSSRSPPLHI SRVLSVETVP LSPSFTWNDV
FENSRKEYVP QNSSDLTGFL EKVDRCNRGL EKLGEFIPFV IEEQIVGYIH KGFTKYLRDF
NDIFTFSQYG GHVTLNMMLD KPEERTRAVA HVIKILGNKG IIPGIRNELY PVKPSFNAPA
FFSIERAAAP YFGLKGYAIH VNGYVERDGQ KFLWIGKRSL AKSTYPGKLD HLVAGGLPHG
ISVCENLVKE CEEEAGISKV LADRAIAVGV VSYMDIDRYC FTRDVLFCYD LELPQDFVPT
NQDGEVDSFR LIPVAQVANV VRKTSFFKDS CSLVIIDFLF RHGLIRPESP GYLDLYRRLR
NGDCS
