NSDHL_DICDI
ID NSDHL_DICDI Reviewed; 328 AA.
AC Q54L85;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating;
DE EC=1.1.1.170 {ECO:0000250|UniProtKB:Q9R1J0};
GN Name=nsdhl; ORFNames=DDB_G0286833;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation
CC of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene
CC cholesterol biosynthesis. {ECO:0000250|UniProtKB:Q15738}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxysteroid-4alpha-carboxylate + NADP(+) = a 3-
CC oxosteroid + CO2 + NADPH; Xref=Rhea:RHEA:34771, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:47788, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:136966; EC=1.1.1.170;
CC Evidence={ECO:0000250|UniProtKB:Q9R1J0};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxysteroid-4alpha-carboxylate + NAD(+) = a 3-
CC oxosteroid + CO2 + NADH; Xref=Rhea:RHEA:34775, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:47788, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:136966; EC=1.1.1.170;
CC Evidence={ECO:0000250|UniProtKB:Q9R1J0};
CC -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from
CC lanosterol: step 4/6.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q15738}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9R1J0}; Single-pass membrane protein
CC {ECO:0000255}. Lipid droplet {ECO:0000250|UniProtKB:Q9R1J0}.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
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DR EMBL; AAFI02000090; EAL64014.1; -; Genomic_DNA.
DR RefSeq; XP_637518.1; XM_632426.1.
DR AlphaFoldDB; Q54L85; -.
DR SMR; Q54L85; -.
DR STRING; 44689.DDB0305146; -.
DR PaxDb; Q54L85; -.
DR EnsemblProtists; EAL64014; EAL64014; DDB_G0286833.
DR GeneID; 8625816; -.
DR KEGG; ddi:DDB_G0286833; -.
DR dictyBase; DDB_G0286833; -.
DR eggNOG; KOG1430; Eukaryota.
DR HOGENOM; CLU_007383_6_1_1; -.
DR InParanoid; Q54L85; -.
DR OMA; SEGVIMN; -.
DR PhylomeDB; Q54L85; -.
DR UniPathway; UPA00770; UER00757.
DR PRO; PR:Q54L85; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:InterPro.
DR GO; GO:0102175; F:3-beta-hydroxysteroid dehydrogenase/C4-decarboxylase activity; IEA:RHEA.
DR GO; GO:0103066; F:4alpha-carboxy-4beta-methyl-5alpha-cholesta-8-en-3beta-ol:NAD(P)+ 3-oxidoreductase (decarboxylating) activity; IEA:UniProtKB-EC.
DR GO; GO:0103067; F:4alpha-carboxy-5alpha-cholesta-8-en-3beta-ol:NAD(P)+ 3-dehydrogenase (decarboxylating) activity; IEA:UniProtKB-EC.
DR GO; GO:0000252; F:C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity; IEA:UniProtKB-EC.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IBA:GO_Central.
DR GO; GO:0047012; F:sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; IEA:UniProtKB-EC.
DR GO; GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 3: Inferred from homology;
KW Cholesterol biosynthesis; Cholesterol metabolism; Endoplasmic reticulum;
KW Lipid biosynthesis; Lipid droplet; Lipid metabolism; Membrane; NAD;
KW Oxidoreductase; Reference proteome; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..328
FT /note="Sterol-4-alpha-carboxylate 3-dehydrogenase,
FT decarboxylating"
FT /id="PRO_0000327832"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 145
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q12068"
FT BINDING 149
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:Q12068"
SQ SEQUENCE 328 AA; 36765 MW; FF402E4213CE9AB3 CRC64;
MKNVFLTGGS GFLGKYIIEE LISNGYKVFA LSRSETSNKV LSQMGATPVM SSLHDEQGLT
EAIKGCDIVI HCAAKLETNS ESVQELYKDN IDATELLFNI CNQSSTSSVS VFCFISSEGV
IMNGENINNA TEDTPYPPIE QLGWYNKSKA ISEQFLLATQ SSMSRMKTIV IRLPLVWGSR
DNVLDYLVGL CNKFQWFWIG GGKNYLSIVH AKNASYGIRL AIEKGDNQDI FHLTDGESVQ
YRKFFTDRFK KKGVSTNKLH MVLPTPIALS LVWIMALIWK LFNLKGLPLL TKTGLIYSSK
NFTINDDKAR LKLGYTNKIN YNQGMDEL
