NPR5_ARATH
ID NPR5_ARATH Reviewed; 491 AA.
AC Q9ZVC2; Q84WN1;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Regulatory protein NPR5;
DE AltName: Full=BTB/POZ domain-containing protein NPR5;
DE AltName: Full=Protein BLADE ON PETIOLE 2;
GN Name=NPR5; Synonyms=BOP2; OrderedLocusNames=At2g41370; ORFNames=F13H10.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND INTERACTION WITH PAN.
RX PubMed=15805484; DOI=10.1105/tpc.104.030536;
RA Hepworth S.R., Zhang Y., McKim S., Li X., Haughn G.W.;
RT "BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning
RT in Arabidopsis.";
RL Plant Cell 17:1434-1448(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=15800002; DOI=10.1242/dev.01815;
RA Norberg M., Holmlund M., Nilsson O.;
RT "The BLADE ON PETIOLE genes act redundantly to control the growth and
RT development of lateral organs.";
RL Development 132:2203-2213(2005).
RN [7]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15634206; DOI=10.1111/j.1365-313x.2004.02296.x;
RA Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.;
RT "An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.";
RL Plant J. 41:304-318(2005).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17601823; DOI=10.1105/tpc.107.051938;
RA Ha C.M., Jun J.H., Nam H.G., Fletcher J.C.;
RT "BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through
RT regulation of LOB domain and adaxial-abaxial polarity genes.";
RL Plant Cell 19:1809-1825(2007).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18339677; DOI=10.1242/dev.012807;
RA McKim S.M., Stenvik G.E., Butenko M.A., Kristiansen W., Cho S.K.,
RA Hepworth S.R., Aalen R.B., Haughn G.W.;
RT "The BLADE-ON-PETIOLE genes are essential for abscission zone formation in
RT Arabidopsis.";
RL Development 135:1537-1546(2008).
RN [11]
RP FUNCTION.
RX PubMed=20610407; DOI=10.1534/genetics.110.118703;
RA Ha C.M., Jun J.H., Fletcher J.C.;
RT "Control of Arabidopsis leaf morphogenesis through regulation of the YABBY
RT and KNOX families of transcription factors.";
RL Genetics 186:197-206(2010).
RN [12]
RP FUNCTION, AND SUBUNIT.
RX PubMed=20118228; DOI=10.1105/tpc.109.070763;
RA Jun J.H., Ha C.M., Fletcher J.C.;
RT "BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in
RT arabidopsis by directly activating ASYMMETRIC LEAVES2.";
RL Plant Cell 22:62-76(2010).
RN [13]
RP FUNCTION.
RX PubMed=20626659; DOI=10.1111/j.1365-313x.2010.04299.x;
RA Xu M., Hu T., McKim S.M., Murmu J., Haughn G.W., Hepworth S.R.;
RT "Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and
RT determinacy in a previously undefined pathway targeting APETALA1 and
RT AGAMOUS-LIKE24.";
RL Plant J. 63:974-989(2010).
RN [14]
RP INDUCTION BY GATA18/HAN, AND TISSUE SPECIFICITY.
RX PubMed=26390296; DOI=10.1371/journal.pgen.1005479;
RA Ding L., Yan S., Jiang L., Zhao W., Ning K., Zhao J., Liu X., Zhang J.,
RA Wang Q., Zhang X.;
RT "HANABA TARANU (HAN) bridges meristem and organ primordia boundaries
RT through PINHEAD, JAGGED, BLADE-ON-PETIOLE2 and CYTOKININ OXIDASE 3 during
RT flower development in Arabidopsis.";
RL PLoS Genet. 11:E1005479-E1005479(2015).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins (By similarity). Acts redundantly with BOP2. BOP1/2 promote
CC leaf and floral meristem fate and determinacy in a pathway targeting
CC AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through
CC direct interaction with TGA factors, including PAN, a direct regulator
CC of AP1. Controls lateral organ fate through positive regulation of
CC adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and
CC through positive regulation of LOB domain-containing genes LOB,
CC LBD6/AS2 and LBD36. Promotes and maintains a developmentally
CC determinate state in leaf cells through the negative regulation of JAG,
CC JGL and class I KNOX genes. Is also involved in nectary development,
CC formation of normal abscission zones and suppression of bract
CC formation. {ECO:0000250, ECO:0000269|PubMed:15800002,
CC ECO:0000269|PubMed:15805484, ECO:0000269|PubMed:17601823,
CC ECO:0000269|PubMed:18339677, ECO:0000269|PubMed:20118228,
CC ECO:0000269|PubMed:20610407, ECO:0000269|PubMed:20626659}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Homodimer or heterodimer with BOP1. Interacts with PAN.
CC {ECO:0000269|PubMed:15805484, ECO:0000269|PubMed:20118228}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15805484}. Nucleus
CC {ECO:0000269|PubMed:15805484}.
CC -!- TISSUE SPECIFICITY: Highly expressed in young floral meristem
CC (PubMed:15805484). Predominantly expressed in the boundary between
CC floral meristem (FM) and sepal primordia (PubMed:26390296).
CC {ECO:0000269|PubMed:15805484, ECO:0000269|PubMed:26390296}.
CC -!- DEVELOPMENTAL STAGE: Expressed during vegetative development in young
CC leaf primordia and at the base of the rosette leaves on the adaxial
CC side. Expressed during reproductive development in young floral buds,
CC and at the base of the sepals and petals.
CC {ECO:0000269|PubMed:15800002}.
CC -!- INDUCTION: Activated by GATA18/HAN. {ECO:0000269|PubMed:26390296}.
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- DISRUPTION PHENOTYPE: Defects in inflorescence and flower development.
CC Bop1 and bop2 double mutant displays leafy petioles, loss of floral
CC organ abscission, and asymmetric flowers subtended by a bract.
CC {ECO:0000269|PubMed:15800002, ECO:0000269|PubMed:15805484,
CC ECO:0000269|PubMed:17601823, ECO:0000269|PubMed:18339677}.
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DR EMBL; AY928830; AAX22759.1; -; mRNA.
DR EMBL; AC005662; AAC78536.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09966.1; -; Genomic_DNA.
DR EMBL; BT002986; AAO22795.1; -; mRNA.
DR EMBL; BT015773; AAU90063.1; -; mRNA.
DR PIR; H84840; H84840.
DR RefSeq; NP_181668.1; NM_129700.3.
DR AlphaFoldDB; Q9ZVC2; -.
DR SMR; Q9ZVC2; -.
DR BioGRID; 4072; 5.
DR IntAct; Q9ZVC2; 4.
DR STRING; 3702.AT2G41370.1; -.
DR iPTMnet; Q9ZVC2; -.
DR PaxDb; Q9ZVC2; -.
DR PRIDE; Q9ZVC2; -.
DR ProteomicsDB; 250515; -.
DR EnsemblPlants; AT2G41370.1; AT2G41370.1; AT2G41370.
DR GeneID; 818735; -.
DR Gramene; AT2G41370.1; AT2G41370.1; AT2G41370.
DR KEGG; ath:AT2G41370; -.
DR Araport; AT2G41370; -.
DR TAIR; locus:2040292; AT2G41370.
DR eggNOG; KOG0504; Eukaryota.
DR HOGENOM; CLU_028148_0_0_1; -.
DR InParanoid; Q9ZVC2; -.
DR OMA; SSMYRPY; -.
DR OrthoDB; 651575at2759; -.
DR PhylomeDB; Q9ZVC2; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9ZVC2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZVC2; baseline and differential.
DR Genevisible; Q9ZVC2; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010434; P:bract formation; IGI:UniProtKB.
DR GO; GO:0010582; P:floral meristem determinacy; IGI:TAIR.
DR GO; GO:0010227; P:floral organ abscission; IGI:UniProtKB.
DR GO; GO:0048439; P:flower morphogenesis; IGI:TAIR.
DR GO; GO:0009864; P:induced systemic resistance, jasmonic acid mediated signaling pathway; IGI:TAIR.
DR GO; GO:0009944; P:polarity specification of adaxial/abaxial axis; IGI:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0009954; P:proximal/distal pattern formation; IGI:TAIR.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR044284; NPR5/6.
DR InterPro; IPR024228; NPR_central_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR46668; PTHR46668; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF11900; DUF3420; 1.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Cytoplasm; Nucleus; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..491
FT /note="Regulatory protein NPR5"
FT /id="PRO_0000407994"
FT DOMAIN 26..116
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REPEAT 254..283
FT /note="ANK 1"
FT REPEAT 284..313
FT /note="ANK 2"
FT REPEAT 318..347
FT /note="ANK 3"
FT REGION 400..491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 400..440
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..476
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 477..491
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 386
FT /note="C -> R (in Ref. 4; AAO22795)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 491 AA; 54001 MW; F2E8A7133B591776 CRC64;
MSNLEESLRS LSLDFLNLLI NGQAFSDVTF SVEGRLVHAH RCILAARSLF FRKFFCGTDS
PQPVTGIDPT QHGSVPASPT RGSTAPAGII PVNSVGYEVF LLLLQFLYSG QVSIVPQKHE
PRPNCGERGC WHTHCSAAVD LALDTLAASR YFGVEQLALL TQKQLASMVE KASIEDVMKV
LIASRKQDMH QLWTTCSHLV AKSGLPPEIL AKHLPIDVVT KIEELRLKSS IARRSLMPHN
HHHDLSVAQD LEDQKIRRMR RALDSSDVEL VKLMVMGEGL NLDESLALHY AVESCSREVV
KALLELGAAD VNYPAGPAGK TPLHIAAEMV SPDMVAVLLD HHADPNVRTV GGITPLDILR
TLTSDFLFKG AVPGLTHIEP NKLRLCLELV QSAAMVISRE EGNNSNNQNN DNNTGIYPHM
NEEHNSGSSG GSNNNLDSRL VYLNLGAGTG QMGPGRDQGD DHNSQREGMS RHHHHHQDPS
TMYHHHHQHH F
