NPR3_ORYSJ
ID NPR3_ORYSJ Reviewed; 589 AA.
AC Q75HA6; Q75HA7; Q75HA8;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=BTB/POZ domain and ankyrin repeat-containing protein NPR3 {ECO:0000305};
DE Short=OsNPR3 {ECO:0000303|PubMed:17309686};
GN Name=NPR3 {ECO:0000303|PubMed:17309686};
GN Synonyms=NH3 {ECO:0000303|PubMed:20561248};
GN OrderedLocusNames=Os03g0667100 {ECO:0000312|EMBL:BAG89750.1},
GN LOC_Os03g46440 {ECO:0000312|EMBL:ABF98080.1};
GN ORFNames=OsJ_12014 {ECO:0000312|EMBL:EAZ28048.1},
GN OSJNBa0056E06.6 {ECO:0000312|EMBL:AAR87166.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), AND FUNCTION.
RX PubMed=17309686; DOI=10.1111/j.1467-7652.2007.00243.x;
RA Yuan Y., Zhong S., Li Q., Zhu Z., Lou Y., Wang L., Wang J., Wang M., Li Q.,
RA Yang D., He Z.;
RT "Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the
RT rice orthologue conferring disease resistance with enhanced herbivore
RT susceptibility.";
RL Plant Biotechnol. J. 5:313-324(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Dongjin;
RA Moon S.-J., Shin D., Kim B.-G., Park S.R., Byun M.-O.;
RT "Oryza sativa japonica group cultivar Dongjin putative NPR1-like protein 3
RT (NPR3) mRNA.";
RL Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP FUNCTION.
RC STRAIN=cv. Kitaake;
RX PubMed=20561248; DOI=10.1111/j.1467-7652.2010.00544.x;
RA Bai W., Chern M., Ruan D., Canlas P.E., Sze-To W.H., Ronald P.C.;
RT "Enhanced disease resistance and hypersensitivity to BTH by introduction of
RT an NH1/OsNPR1 paralog.";
RL Plant Biotechnol. J. 9:205-215(2011).
RN [10]
RP INTERACTION WITH TGA2.1; TGA2.2; TGA2.3; LG2; TGAL1; TGAL4; NRR; RH1; RH2
RP AND RH3.
RX PubMed=24919709; DOI=10.1186/1471-2164-15-461;
RA Chern M., Bai W., Ruan D., Oh T., Chen X., Ronald P.C.;
RT "Interaction specificity and coexpression of rice NPR1 homologs 1 and 3
RT (NH1 and NH3), TGA transcription factors and negative regulator of
RT resistance (NRR) proteins.";
RL BMC Genomics 15:461-461(2014).
CC -!- FUNCTION: Involved in defense response against the bacterial blight
CC disease caused by Xanthomonas oryzae pv. oryzae (Xoo). Plants
CC expressing an NPR3/NH3 transgene driven by its native promoter show
CC enhanced resistance to the Xoo pathogen, and exhibit elevated
CC sensitivity to benzothiadiazole (BTH) treatment and enhanced induction
CC of defense-related genes upon treatment with BTH (PubMed:20561248).
CC Intriguingly, constitutive over-expression of NPR3/NH3 with a ubiquitin
CC promoter does not confer disease resistance to Xoo (PubMed:17309686).
CC {ECO:0000269|PubMed:17309686, ECO:0000269|PubMed:20561248}.
CC -!- SUBUNIT: Interacts with TGA2.1, TGA2.2, TGA2.3, LG2, TGAL1, TGAL4, NRR,
CC RH1, RH2 and RH3. {ECO:0000269|PubMed:24919709}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q75HA6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q75HA6-2; Sequence=VSP_058476, VSP_058477;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR87167.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ450951; ABE11617.1; -; mRNA.
DR EMBL; DQ450952; ABE11618.1; -; Genomic_DNA.
DR EMBL; HM991170; AEF30413.1; -; mRNA.
DR EMBL; AC135792; AAR87166.1; -; Genomic_DNA.
DR EMBL; AC135792; AAR87167.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC135792; AAR87168.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98078.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98080.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12764.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS85647.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS85650.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ28048.1; -; Genomic_DNA.
DR EMBL; AK065363; BAG89488.1; -; mRNA.
DR EMBL; AK065952; BAG89750.1; -; mRNA.
DR RefSeq; XP_015632015.1; XM_015776529.1. [Q75HA6-1]
DR RefSeq; XP_015632016.1; XM_015776530.1.
DR AlphaFoldDB; Q75HA6; -.
DR SMR; Q75HA6; -.
DR STRING; 4530.OS03T0667100-01; -.
DR PaxDb; Q75HA6; -.
DR PRIDE; Q75HA6; -.
DR EnsemblPlants; Os03t0667100-01; Os03t0667100-01; Os03g0667100. [Q75HA6-1]
DR EnsemblPlants; Os03t0667100-02; Os03t0667100-02; Os03g0667100. [Q75HA6-2]
DR GeneID; 4333666; -.
DR Gramene; Os03t0667100-01; Os03t0667100-01; Os03g0667100. [Q75HA6-1]
DR Gramene; Os03t0667100-02; Os03t0667100-02; Os03g0667100. [Q75HA6-2]
DR KEGG; osa:4333666; -.
DR eggNOG; KOG0504; Eukaryota.
DR InParanoid; Q75HA6; -.
DR OMA; SQCIQRI; -.
DR OrthoDB; 631377at2759; -.
DR PlantReactome; R-OSA-6788019; Salicylic acid signaling.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q75HA6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IBA:GO_Central.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IEA:InterPro.
DR GO; GO:0009862; P:systemic acquired resistance, salicylic acid mediated signaling pathway; IEA:InterPro.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR044292; NPR.
DR InterPro; IPR021094; NPR1/NIM1-like_C.
DR InterPro; IPR024228; NPR_central_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR46475; PTHR46475; 1.
DR Pfam; PF13637; Ank_4; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF11900; DUF3420; 1.
DR Pfam; PF12313; NPR1_like_C; 1.
DR SMART; SM00248; ANK; 2.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ANK repeat; Plant defense; Reference proteome;
KW Repeat.
FT CHAIN 1..589
FT /note="BTB/POZ domain and ankyrin repeat-containing protein
FT NPR3"
FT /id="PRO_0000437002"
FT DOMAIN 52..137
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REPEAT 260..290
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 292..319
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 323..352
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 555..589
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 559..576
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 573
FT /note="R -> S (in isoform 2)"
FT /id="VSP_058476"
FT VAR_SEQ 574..589
FT /note="Missing (in isoform 2)"
FT /id="VSP_058477"
SQ SEQUENCE 589 AA; 65047 MW; 4B3F1F541F1CC56F CRC64;
METSTISFSS SSPPSPPPPQ PAPGDIDAVS LGRLSRNLEN LLDPAFLNCA DAEIVLASGG
GDPGGGAVVG VHRCILAARS RFFYDHFSSA PAPAPATAGD KPQLDLDGLV PGGRHIGRDA
LVAVLSYLYT GRLRSAPPEA AACLDDGCSH DACRPAIDFV VESTYAASGF QISELVSLFQ
RRLSDFVNKA LAEDILPILV VASTCHLPEL LNQCIQRVAN SNLDNRYLEK RLPDDLYAKL
KEFRVPDEPH SGILDPEHEK RVRNIHKALD SDDVDLVGML LKESPVTLDD AFAIHYAAAY
CEPKVLAELL KLESANVNLK NSSGYTPLHM ACMRREPDII VSLIEKGASV LERTQDGRDA
LTICKRLTRE KDRNEKSEKC KERSKAYLCI GVLQQEIKRR PQILEDQMSA EESIATPLLV
DNFHMRLLNL ENRVAFARIF FPSEAKLVMR IAQADSTQEF AGLTSANFSK LKEVDLNETP
TMQNRRLRER LDALTKTVEL GRRYFPHCSE VLDKFLNEES TDLILLESGT AEDQQTKRMR
FSELREDVRK AFTKDKAAGA AISSSTSASS SPRYETKLRP GNKKGKLSR
