NPR3_ARATH
ID NPR3_ARATH Reviewed; 586 AA.
AC Q8L746; Q9FHE3;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Regulatory protein NPR3;
DE AltName: Full=BTB/POZ domain-containing protein NPR3;
GN Name=NPR3; OrderedLocusNames=At5g45110; ORFNames=K17O22_11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15634206; DOI=10.1111/j.1365-313x.2004.02296.x;
RA Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.;
RT "An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.";
RL Plant J. 41:304-318(2005).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH TGA FACTORS,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=17076807; DOI=10.1111/j.1365-313x.2006.02903.x;
RA Zhang Y., Cheng Y.T., Qu N., Zhao Q., Bi D., Li X.;
RT "Negative regulation of defense responses in Arabidopsis by two NPR1
RT paralogs.";
RL Plant J. 48:647-656(2006).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins (By similarity). Involved in the regulation of basal defense
CC responses against pathogens. {ECO:0000250,
CC ECO:0000269|PubMed:17076807}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with TGA2, TGA3, TGA5 and TGA6. Interacts with TGA2
CC in vivo in the nucleus. {ECO:0000269|PubMed:17076807}.
CC -!- INTERACTION:
CC Q8L746; Q9LUX9: At5g58800; NbExp=3; IntAct=EBI-4441365, EBI-4445291;
CC Q8L746; Q9FNZ5: NIMIN-1; NbExp=4; IntAct=EBI-4441365, EBI-541099;
CC Q8L746; Q9LUA3: NIMIN-2; NbExp=3; IntAct=EBI-4441365, EBI-541107;
CC Q8L746; Q9FNZ4: NIMIN-3; NbExp=3; IntAct=EBI-4441365, EBI-541115;
CC Q8L746; P93002: NPR1; NbExp=2; IntAct=EBI-4441365, EBI-1392127;
CC Q8L746; Q8L746: NPR3; NbExp=4; IntAct=EBI-4441365, EBI-4441365;
CC Q8L746; Q5ICL9: NPR4; NbExp=5; IntAct=EBI-4441365, EBI-1392093;
CC Q8L746; Q93ZX1: RFC4; NbExp=3; IntAct=EBI-4441365, EBI-4470690;
CC Q8L746; Q39237: TGA1; NbExp=3; IntAct=EBI-4441365, EBI-541351;
CC Q8L746; P43273: TGA2; NbExp=9; IntAct=EBI-4441365, EBI-541307;
CC Q8L746; Q39234: TGA3; NbExp=6; IntAct=EBI-4441365, EBI-541366;
CC Q8L746; Q39163: TGA5; NbExp=4; IntAct=EBI-4441365, EBI-541381;
CC Q8L746; Q93XM6: TGA9; NbExp=3; IntAct=EBI-4441365, EBI-1237844;
CC Q8L746; Q94AI7: TPL; NbExp=3; IntAct=EBI-4441365, EBI-2119299;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17076807}.
CC -!- INDUCTION: Up-regulated following pathogen challenge or salicylic acid
CC (SA) treatment. {ECO:0000269|PubMed:17076807}.
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- DISRUPTION PHENOTYPE: Elevated PR-1 expression level and enhanced
CC resistance to Hyaloperonospora parasitica.
CC {ECO:0000269|PubMed:17076807}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09496.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB019224; BAB09496.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95205.1; -; Genomic_DNA.
DR EMBL; AY139763; AAM98084.1; -; mRNA.
DR EMBL; BT004537; AAO42783.1; -; mRNA.
DR RefSeq; NP_199324.2; NM_123879.3.
DR AlphaFoldDB; Q8L746; -.
DR SMR; Q8L746; -.
DR BioGRID; 19794; 18.
DR DIP; DIP-59898N; -.
DR IntAct; Q8L746; 18.
DR STRING; 3702.AT5G45110.1; -.
DR PaxDb; Q8L746; -.
DR PRIDE; Q8L746; -.
DR ProteomicsDB; 250547; -.
DR EnsemblPlants; AT5G45110.1; AT5G45110.1; AT5G45110.
DR GeneID; 834545; -.
DR Gramene; AT5G45110.1; AT5G45110.1; AT5G45110.
DR KEGG; ath:AT5G45110; -.
DR Araport; AT5G45110; -.
DR TAIR; locus:2153192; AT5G45110.
DR eggNOG; KOG0504; Eukaryota.
DR HOGENOM; CLU_034895_1_0_1; -.
DR InParanoid; Q8L746; -.
DR OrthoDB; 631377at2759; -.
DR PhylomeDB; Q8L746; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8L746; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8L746; baseline and differential.
DR Genevisible; Q8L746; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:1901149; F:salicylic acid binding; IDA:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IEA:InterPro.
DR GO; GO:0009627; P:systemic acquired resistance; IGI:TAIR.
DR GO; GO:0009862; P:systemic acquired resistance, salicylic acid mediated signaling pathway; IEA:InterPro.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR044292; NPR.
DR InterPro; IPR021094; NPR1/NIM1-like_C.
DR InterPro; IPR024228; NPR_central_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR46475; PTHR46475; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF11900; DUF3420; 1.
DR Pfam; PF12313; NPR1_like_C; 1.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Nucleus; Phosphoprotein; Plant defense; Reference proteome;
KW Repeat; Ubl conjugation pathway.
FT CHAIN 1..586
FT /note="Regulatory protein NPR3"
FT /id="PRO_0000407992"
FT DOMAIN 60..135
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REPEAT 261..291
FT /note="ANK 1"
FT REPEAT 293..320
FT /note="ANK 2"
FT REPEAT 324..353
FT /note="ANK 3"
FT REGION 554..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..586
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 10
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P93002"
SQ SEQUENCE 586 AA; 66061 MW; E0C065A1F4A9F692 CRC64;
MATLTEPSSS LSFTSSHFSY GSIGSNHFSS SSASNPEVVS LTKLSSNLEQ LLSNSDCDYS
DAEIIVDGVP VGVHRCILAA RSKFFQDLFK KEKKISKTEK PKYQLREMLP YGAVAHEAFL
YFLSYIYTGR LKPFPLEVST CVDPVCSHDC CRPAIDFVVQ LMYASSVLQV PELVSSFQRR
LCNFVEKTLV ENVLPILMVA FNCKLTQLLD QCIERVARSD LYRFCIEKEV PPEVAEKIKQ
LRLISPQDEE TSPKISEKLL ERIGKILKAL DSDDVELVKL LLTESDITLD QANGLHYSVV
YSDPKVVAEI LALDMGDVNY RNSRGYTVLH FAAMRREPSI IISLIDKGAN ASEFTSDGRS
AVNILRRLTN PKDYHTKTAK GRESSKARLC IDILEREIRK NPMVLDTPMC SISMPEDLQM
RLLYLEKRVG LAQLFFPTEA KVAMDIGNVE GTSEFTGLSP PSSGLTGNLS QVDLNETPHM
QTQRLLTRMV ALMKTVETGR RFFPYGSEVL DKYMAEYIDD DILDDFHFEK GSTHERRLKR
MRYRELKDDV QKAYSKDKES KIARSCLSAS SSPSSSSIRD DLHNTT
