ID   NHAD_VIBPH              Reviewed;         414 AA.
AC   O66163;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Na(+)/H(+) antiporter NhaD;
DE   AltName: Full=Sodium/proton antiporter NhaD;
GN   Name=nhaD;
OS   Vibrio parahaemolyticus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   SUBCELLULAR LOCATION, AND GENE NAME.
RC   STRAIN=AQ3334;
RX   PubMed=9518619; DOI=10.1016/s0005-2736(97)00223-x;
RA   Nozaki K., Kuroda T., Mizushima T., Tsuchiya T.;
RT   "A new Na+/H+ antiporter, NhaD, of Vibrio parahaemolyticus.";
RL   Biochim. Biophys. Acta 1369:213-220(1998).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. Can also transport lithium.
CC       {ECO:0000269|PubMed:9518619}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.5-9.0. Inactive at pH 7.0 or 7.5.
CC         {ECO:0000269|PubMed:9518619};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:9518619};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:9518619}.
CC   -!- SIMILARITY: Belongs to the NhaD Na(+)/H(+) (TC 2.A.62) antiporter
CC       family. {ECO:0000305}.
CC   -!- CAUTION: The N-terminus is shorter than in orthologs. {ECO:0000305}.
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DR   EMBL; AB006008; BAA25994.1; -; Genomic_DNA.
DR   AlphaFoldDB; O66163; -.
DR   TCDB; 2.A.62.1.1; the nhad na(+):h(+) antiporter (nhad) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR004680; Cit_transptr-like_dom.
DR   InterPro; IPR004672; Na(+)/H(+)_antiporter_NhaD.
DR   InterPro; IPR045016; NhaD-like.
DR   PANTHER; PTHR43269; PTHR43269; 1.
DR   Pfam; PF03600; CitMHS; 1.
DR   TIGRFAMs; TIGR00775; NhaD; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..414
FT                   /note="Na(+)/H(+) antiporter NhaD"
FT                   /id="PRO_0000423662"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..309
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        364..384
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..412
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   414 AA;  45337 MW;  8F319654C42105F2 CRC64;
     MRKSKPVLLA AGLIWILIGY TFAQHHQQDV AKAALEHNLL EYAELLLFLL VAMTYINAME
     ERKLFDALQA WMVGKGFGFK KLFWLTGFLA FVISPIADNL TTALLMCAVV MKVSGDNPRF
     VNLACINIVI AANAGGAFSP FGDITTLMVW QAGHVRFSEF MPLFVPSLIN YVVPAFLMAL
     FVPNTKPNTI HEHVELKRGA RRIVLLFVLT IATAVSFHAV LHFPPVVGMM MGLAYLQFFG
     YFLRKTLKHS LAKKAAMAIA NGDDHALKRL GSVVPFDVFH RVSRAEWDTL LFFYGVVMCV
     GGLSLLGYLE LVSNVMYTQW NPVWANVMVG VLSAIVDNIP VMFAVLTMDP SMSTGNWLLV
     TLTAGVGGSL LSIGSAAGVA LMGAARGQYT FFGHLKWTPV IALGYAPVLP LICG