NFYA4_ARATH
ID NFYA4_ARATH Reviewed; 198 AA.
AC Q8VY64; O64585;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Nuclear transcription factor Y subunit A-4;
DE Short=AtNF-YA-4;
GN Name=NFYA4; OrderedLocusNames=At2g34720; ORFNames=T29F13.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11250072; DOI=10.1016/s0378-1119(01)00323-7;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of the CCAAT-binding NF-Y subunits in Arabidopsis thaliana.";
RL Gene 264:173-185(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11867211; DOI=10.1016/s0378-1119(01)00833-2;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding
RT nuclear factor Y subunits.";
RL Gene 283:41-48(2002).
CC -!- FUNCTION: Stimulates the transcription of various genes by recognizing
CC and binding to a CCAAT motif in promoters. {ECO:0000250}.
CC -!- SUBUNIT: Heterotrimeric transcription factor composed of three
CC components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and
CC dimerize for NF-YA association and DNA binding (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q8VY64; A9LNK9: CPSF30; NbExp=4; IntAct=EBI-4461713, EBI-962511;
CC Q8VY64; O23310: NFYB3; NbExp=3; IntAct=EBI-4461713, EBI-4452064;
CC Q8VY64; Q8L4B2: NFYC9; NbExp=4; IntAct=EBI-4461713, EBI-2466050;
CC Q8VY64; Q9S7W5: TCP13; NbExp=3; IntAct=EBI-4461713, EBI-4424877;
CC Q8VY64; O64647: TCP9; NbExp=3; IntAct=EBI-4461713, EBI-9838721;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in stems, caulines, and senescent
CC flowers. {ECO:0000269|PubMed:11250072}.
CC -!- SIMILARITY: Belongs to the NFYA/HAP2 subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00966}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC16262.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003096; AAC16262.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09013.1; -; Genomic_DNA.
DR EMBL; AY072414; AAL62406.1; -; mRNA.
DR EMBL; AY114711; AAM48030.1; -; mRNA.
DR PIR; T01363; T01363.
DR RefSeq; NP_850235.1; NM_179904.5.
DR AlphaFoldDB; Q8VY64; -.
DR SMR; Q8VY64; -.
DR BioGRID; 3383; 34.
DR IntAct; Q8VY64; 27.
DR STRING; 3702.AT2G34720.1; -.
DR iPTMnet; Q8VY64; -.
DR PaxDb; Q8VY64; -.
DR PRIDE; Q8VY64; -.
DR ProteomicsDB; 251160; -.
DR EnsemblPlants; AT2G34720.1; AT2G34720.1; AT2G34720.
DR GeneID; 818037; -.
DR Gramene; AT2G34720.1; AT2G34720.1; AT2G34720.
DR KEGG; ath:AT2G34720; -.
DR Araport; AT2G34720; -.
DR TAIR; locus:2061534; AT2G34720.
DR eggNOG; KOG1561; Eukaryota.
DR HOGENOM; CLU_065504_1_0_1; -.
DR InParanoid; Q8VY64; -.
DR OMA; RHMHALR; -.
DR OrthoDB; 1411373at2759; -.
DR PhylomeDB; Q8VY64; -.
DR PRO; PR:Q8VY64; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VY64; baseline and differential.
DR Genevisible; Q8VY64; AT.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR018362; CCAAT-binding_factor_CS.
DR InterPro; IPR001289; NFYA.
DR PANTHER; PTHR12632; PTHR12632; 1.
DR Pfam; PF02045; CBFB_NFYA; 1.
DR PRINTS; PR00616; CCAATSUBUNTB.
DR SMART; SM00521; CBF; 1.
DR PROSITE; PS00686; NFYA_HAP2_1; 1.
DR PROSITE; PS51152; NFYA_HAP2_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..198
FT /note="Nuclear transcription factor Y subunit A-4"
FT /id="PRO_0000198774"
FT DNA_BIND 131..156
FT /note="NFYA/HAP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 101..124
FT /note="Subunit association domain (SAD)"
FT COMPBIAS 10..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..188
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 198 AA; 22259 MW; 665ACB070E64D00C CRC64;
MTSSVHELSD NNESHAKKER PDSQTRPQVP SGRSSESIDT NSVYSEPMAH GLYPYPDPYY
RSVFAQQAYL PHPYPGVQLQ LMGMQQPGVP LQCDAVEEPV FVNAKQYHGI LRRRQSRAKL
EARNRAIKAK KPYMHESRHL HAIRRPRGCG GRFLNAKKEN GDHKEEEEAT SDENTSEASS
SLRSEKLAMA TSGPNGRS
