NEK1_ARATH
ID NEK1_ARATH Reviewed; 612 AA.
AC Q9SLI2; Q9SLI3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Serine/threonine-protein kinase Nek1;
DE EC=2.7.11.1;
DE AltName: Full=NimA-related protein kinase 1;
DE Short=AtNek1;
GN Name=NEK1; OrderedLocusNames=At1g54510; ORFNames=F20D21.32/F20D21.33;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17886359; DOI=10.1111/j.1365-313x.2007.03161.x;
RA Vigneault F., Lachance D., Cloutier M., Pelletier G., Levasseur C.,
RA Seguin A.;
RT "Members of the plant NIMA-related kinases are involved in organ
RT development and vascularization in poplar, Arabidopsis and rice.";
RL Plant J. 51:575-588(2007).
CC -!- FUNCTION: May be involved in plant development processes.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr
CC protein kinase family. NIMA subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25629.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=AAD25631.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=BX816613; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AC005287; AAD25629.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC005287; AAD25631.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33111.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33112.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33113.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59722.1; -; Genomic_DNA.
DR EMBL; BX816613; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A96587; A96587.
DR PIR; B96587; B96587.
DR RefSeq; NP_001185224.1; NM_001198295.1.
DR RefSeq; NP_001185225.1; NM_001198296.1.
DR RefSeq; NP_001319226.1; NM_001333653.1.
DR RefSeq; NP_175853.1; NM_104329.5.
DR AlphaFoldDB; Q9SLI2; -.
DR SMR; Q9SLI2; -.
DR STRING; 3702.AT1G54510.1; -.
DR PaxDb; Q9SLI2; -.
DR PRIDE; Q9SLI2; -.
DR ProteomicsDB; 250825; -.
DR EnsemblPlants; AT1G54510.1; AT1G54510.1; AT1G54510.
DR EnsemblPlants; AT1G54510.2; AT1G54510.2; AT1G54510.
DR EnsemblPlants; AT1G54510.3; AT1G54510.3; AT1G54510.
DR EnsemblPlants; AT1G54510.4; AT1G54510.4; AT1G54510.
DR GeneID; 841893; -.
DR Gramene; AT1G54510.1; AT1G54510.1; AT1G54510.
DR Gramene; AT1G54510.2; AT1G54510.2; AT1G54510.
DR Gramene; AT1G54510.3; AT1G54510.3; AT1G54510.
DR Gramene; AT1G54510.4; AT1G54510.4; AT1G54510.
DR KEGG; ath:AT1G54510; -.
DR Araport; AT1G54510; -.
DR TAIR; locus:2020148; AT1G54510.
DR eggNOG; KOG0589; Eukaryota.
DR HOGENOM; CLU_000288_128_3_1; -.
DR InParanoid; Q9SLI2; -.
DR OMA; VEKACYV; -.
DR OrthoDB; 317437at2759; -.
DR PhylomeDB; Q9SLI2; -.
DR PRO; PR:Q9SLI2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SLI2; baseline and differential.
DR Genevisible; Q9SLI2; AT.
DR GO; GO:0055028; C:cortical microtubule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0007017; P:microtubule-based process; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..612
FT /note="Serine/threonine-protein kinase Nek1"
FT /id="PRO_0000314037"
FT DOMAIN 4..258
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 503..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 503..532
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 129
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 10..18
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 612 AA; 68836 MW; AB45106F89F6EEAD CRC64;
MEQYEFLEQI GKGSFGSALL VRHKHEKKKY VLKKIRLARQ TQRTRRSAHQ EMELISKMRH
PFIVEYKDSW VEKACYVCIV IGYCEGGDMA QAIKKSNGVH FQEEKLCKWL VQLLMGLEYL
HSNHILHRDV KCSNIFLTKE QDIRLGDFGL AKILTSDDLT SSVVGTPSYM CPELLADIPY
GSKSDIWSLG CCIYEMAYLK PAFKAFDMQA LINKINKTIV SPLPAKYSGP FRGLVKSMLR
KNPEVRPSAS DLLRHPHLQP YVLDVKLRLN NLRRKTLPPE LPSSKRIMKK AHFSEPAVTC
PAFGERQHRS LWNDRALNPE AEEDTASSIK CISRRISDLS IESSSKGTLI CKQVSSSACK
VSKYPLAKSS VTSRRIMETG RRSDHLHPVS GGGTTSKIIP SARRTSLPLT KRATNQEVAA
YNPIVGILQN VKSPEYSINE PQVDKIAIFP LAPYEQDIFF TPMQRKTSSK SSSVSDRSIT
KDKCTVQTHT TWQGIQLNMV DNISDGSSSS DQNATAGASS HTTSSSSRRC RFDPSSYRQR
ADALEGLLEF SARLLQEGRY DELNVLLKPF GPGKVSPRET AIWIAKSLKE NRDKTKMVDL
NVSREIPHVG LL
