NCKX3_MOUSE
ID NCKX3_MOUSE Reviewed; 645 AA.
AC Q99PD7; B1AWL7; Q99JR2; Q99PD8;
DT 13-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Sodium/potassium/calcium exchanger 3;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein 3 {ECO:0000303|PubMed:15489334};
DE AltName: Full=Solute carrier family 24 member 3;
DE Flags: Precursor;
GN Name=Slc24a3; Synonyms=Nckx3 {ECO:0000303|PubMed:15489334};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC STRAIN=129/SvJ, and CD-1; TISSUE=Brain, and Embryonic stem cell;
RX PubMed=11294880; DOI=10.1074/jbc.m102314200;
RA Kraev A., Quednau B.D., Leach S., Li X.-F., Dong H., Winkfein R.,
RA Perizzolo M., Cai X., Yang R., Philipson K.D., Lytton J.;
RT "Molecular cloning of a third member of the potassium-dependent sodium-
RT calcium exchanger gene family, NCKX3.";
RL J. Biol. Chem. 276:23161-23172(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 323-645.
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=22677781; DOI=10.1159/000337493;
RA Hu P., Lacruz R.S., Smith C.E., Smith S.M., Kurtz I., Paine M.L.;
RT "Expression of the sodium/calcium/potassium exchanger, NCKX4, in
RT ameloblasts.";
RL Cells Tissues Organs 196:501-509(2012).
CC -!- FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+)
CC and 1 K(+) in exchange for 4 Na(+). {ECO:0000269|PubMed:11294880}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in)
CC + 4 Na(+)(out); Xref=Rhea:RHEA:69967, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:29103, ChEBI:CHEBI:29108;
CC Evidence={ECO:0000250|UniProtKB:Q9HC58};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9HC58};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Abundant in the brain. Highest levels found in
CC selected thalamic nuclei, hippocampal CA1 neurons and in layer IV of
CC the cerebral cortex. Expressed in dental tissues.
CC {ECO:0000269|PubMed:22677781}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
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DR EMBL; AF314821; AAG60049.1; -; mRNA.
DR EMBL; AF314822; AAG60050.1; -; Genomic_DNA.
DR EMBL; AL772192; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL808117; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL824715; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL831783; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC005742; AAH05742.1; -; mRNA.
DR CCDS; CCDS89560.1; -.
DR AlphaFoldDB; Q99PD7; -.
DR STRING; 10090.ENSMUSP00000105634; -.
DR GlyGen; Q99PD7; 2 sites.
DR iPTMnet; Q99PD7; -.
DR PhosphoSitePlus; Q99PD7; -.
DR MaxQB; Q99PD7; -.
DR PaxDb; Q99PD7; -.
DR PRIDE; Q99PD7; -.
DR ProteomicsDB; 252926; -.
DR Antibodypedia; 65262; 115 antibodies from 20 providers.
DR Ensembl; ENSMUST00000110007; ENSMUSP00000105634; ENSMUSG00000063873.
DR UCSC; uc008mrq.1; mouse.
DR MGI; MGI:2137513; Slc24a3.
DR VEuPathDB; HostDB:ENSMUSG00000063873; -.
DR eggNOG; KOG1307; Eukaryota.
DR GeneTree; ENSGT01030000234532; -.
DR InParanoid; Q99PD7; -.
DR PhylomeDB; Q99PD7; -.
DR TreeFam; TF318759; -.
DR Reactome; R-MMU-425561; Sodium/Calcium exchangers.
DR ChiTaRS; Slc24a3; mouse.
DR PRO; PR:Q99PD7; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q99PD7; protein.
DR Bgee; ENSMUSG00000063873; Expressed in bed nucleus of stria terminalis and 232 other tissues.
DR ExpressionAtlas; Q99PD7; baseline and differential.
DR Genevisible; Q99PD7; MM.
DR GO; GO:0071944; C:cell periphery; IDA:ARUK-UCL.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; IDA:MGI.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0030282; P:bone mineralization; IMP:ARUK-UCL.
DR GO; GO:0070588; P:calcium ion transmembrane transport; ISO:MGI.
DR GO; GO:0006812; P:cation transport; ISO:MGI.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IMP:ARUK-UCL.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:ARUK-UCL.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:ARUK-UCL.
DR GO; GO:0071805; P:potassium ion transmembrane transport; ISO:MGI.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISO:MGI.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR InterPro; IPR030248; SLC24A3.
DR PANTHER; PTHR10846; PTHR10846; 1.
DR PANTHER; PTHR10846:SF22; PTHR10846:SF22; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 1: Evidence at protein level;
KW Antiport; Calcium; Calcium transport; Cell membrane; Glycoprotein;
KW Ion transport; Membrane; Phosphoprotein; Potassium; Potassium transport;
KW Reference proteome; Repeat; Signal; Sodium; Sodium transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..43
FT /evidence="ECO:0000255"
FT CHAIN 44..645
FT /note="Sodium/potassium/calcium exchanger 3"
FT /id="PRO_0000019371"
FT TOPO_DOM 44..106
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..181
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..209
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..234
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..486
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 487..507
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 508..512
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 513..533
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 534..551
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 573..582
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 583..603
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 604..617
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 618..638
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 639..645
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REPEAT 148..188
FT /note="Alpha-1"
FT REPEAT 520..551
FT /note="Alpha-2"
FT REGION 379..398
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 404..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 406..437
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 422
FT /note="N -> S (in Ref. 1; AAG60049)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 645 AA; 71942 MW; FA57D33BA1D14B84 CRC64;
MPPPGDQDCA RRRSRRRRRD LLLSQLCFLA SVALLLWSLS SLREQKELDL MDLIGEDRKW
MVGRKLMQVN DTLTSEDAGL QSSKNCTEPA LHEFPRDIFS NEDRRQGAVV LHVLCAMYMF
YALAIVCDDF FVPSLEKICE RLHLSEDVAG ATFMAAGSSA PELFTSVIGV FITKGDVGVG
TIVGSAVFNI LCIIGVCGLF AGQVVALSSW CLLRDSIYYT LSVVALIVFI YDEKVSWWES
LVLVLMYLIY IVIMKYNACI HQCFERRTKG AGNMVNGLAN NAEIDDSSNC DATVVLLKKA
NFHRKASVIM VDELLSAYPH QLSFSEAGLR IMITSHFPPK TRLSMASRML INERQRLINS
RAYTNGESEV AIKIPIKHTV ENGTGPSSAP DRGVNGTRRD DIVAETDNET ENENEDNENN
ENDEEEEEDE DDDEGPYTPF DPPSGKLETV KWAFTWPLSF VLYFTVPNCN KPHWEKWFMV
TFASSTLWIA AFSYMMVWMV TIIGYTLGIP DVIMGITFLA AGTSVPDCMA SLIVARQGMG
DMAVSNSIGS NVFDILIGLG LPWALQTLAV DYGSYIRLNS RGLIYSVGLL LASVFVTVFG
VHLNKWQLDK KLGCGCLFLY GVFLCFSIMT EFNVFTFVNL PMCGD
