NCKX2_RAT
ID NCKX2_RAT Reviewed; 670 AA.
AC O54701; O54706;
DT 13-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Sodium/potassium/calcium exchanger 2;
DE AltName: Full=Na(+)/K(+)/Ca(2+)-exchange protein 2;
DE AltName: Full=Retinal cone Na-Ca+K exchanger;
DE AltName: Full=Solute carrier family 24 member 2;
GN Name=Slc24a2; Synonyms=Nckx2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TRANSPORTER
RP ACTIVITY, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain cortex;
RX PubMed=9461611; DOI=10.1074/jbc.273.7.4155;
RA Tsoi M., Rhee K.-H., Bungard D., Li X.-F., Lee S.-L., Auer R.N., Lytton J.;
RT "Molecular cloning of a novel potassium-dependent sodium-calcium exchanger
RT from rat brain.";
RL J. Biol. Chem. 273:4155-4162(1998).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND SER-341, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+)
CC and 1 K(+) in exchange for 4 Na(+) (PubMed:9461611). Required for
CC learming and memory by regulating neuronal Ca(2+), which is essential
CC for the development of synaptic plasticity (By similarity).
CC {ECO:0000250|UniProtKB:Q8BUN9, ECO:0000269|PubMed:9461611}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in)
CC + 4 Na(+)(out); Xref=Rhea:RHEA:69967, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:29103, ChEBI:CHEBI:29108;
CC Evidence={ECO:0000305|PubMed:9461611};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9461611};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O54701-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O54701-2; Sequence=VSP_006165;
CC -!- TISSUE SPECIFICITY: Expressed abundantly in all regions of the brain
CC and weakly in the eye, large intestine and adrenal tissue.
CC {ECO:0000269|PubMed:9461611}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
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DR EMBL; AF021923; AAC19405.1; -; mRNA.
DR EMBL; AF027506; AAC19404.1; -; mRNA.
DR RefSeq; NP_113931.1; NM_031743.2. [O54701-1]
DR AlphaFoldDB; O54701; -.
DR SMR; O54701; -.
DR BioGRID; 250001; 1.
DR STRING; 10116.ENSRNOP00000010827; -.
DR TCDB; 2.A.19.4.2; the ca(2+):cation antiporter (caca) family.
DR GlyGen; O54701; 1 site.
DR iPTMnet; O54701; -.
DR PhosphoSitePlus; O54701; -.
DR PaxDb; O54701; -.
DR PRIDE; O54701; -.
DR Ensembl; ENSRNOT00000010827; ENSRNOP00000010827; ENSRNOG00000008169. [O54701-1]
DR Ensembl; ENSRNOT00000083373; ENSRNOP00000074180; ENSRNOG00000008169. [O54701-2]
DR GeneID; 84550; -.
DR KEGG; rno:84550; -.
DR UCSC; RGD:628650; rat. [O54701-1]
DR CTD; 25769; -.
DR RGD; 628650; Slc24a2.
DR eggNOG; KOG1307; Eukaryota.
DR GeneTree; ENSGT01030000234532; -.
DR HOGENOM; CLU_007948_5_1_1; -.
DR InParanoid; O54701; -.
DR OMA; TIPDCRY; -.
DR OrthoDB; 1168500at2759; -.
DR PhylomeDB; O54701; -.
DR TreeFam; TF318759; -.
DR Reactome; R-RNO-425561; Sodium/Calcium exchangers.
DR PRO; PR:O54701; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000008169; Expressed in Ammon's horn and 3 other tissues.
DR Genevisible; O54701; RN.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0031226; C:intrinsic component of plasma membrane; IMP:RGD.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0005262; F:calcium channel activity; ISO:RGD.
DR GO; GO:0008273; F:calcium, potassium:sodium antiporter activity; IDA:RGD.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0070509; P:calcium ion import; ISO:RGD.
DR GO; GO:0098703; P:calcium ion import across plasma membrane; ISO:RGD.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:RGD.
DR GO; GO:0006816; P:calcium ion transport; ISO:RGD.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; ISO:RGD.
DR GO; GO:0071486; P:cellular response to high light intensity; ISO:RGD.
DR GO; GO:0034220; P:ion transmembrane transport; ISO:RGD.
DR GO; GO:0007612; P:learning; ISO:RGD.
DR GO; GO:0060292; P:long-term synaptic depression; ISO:RGD.
DR GO; GO:0060291; P:long-term synaptic potentiation; ISO:RGD.
DR GO; GO:0007613; P:memory; ISO:RGD.
DR GO; GO:0071805; P:potassium ion transmembrane transport; ISO:RGD.
DR GO; GO:0065003; P:protein-containing complex assembly; IMP:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISO:RGD.
DR GO; GO:0007601; P:visual perception; IEA:InterPro.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004481; K/Na/Ca-exchanger.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR InterPro; IPR030236; SLC24A2.
DR PANTHER; PTHR10846; PTHR10846; 1.
DR PANTHER; PTHR10846:SF41; PTHR10846:SF41; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00367; TIGR00367; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Antiport; Calcium; Calcium transport; Cell membrane;
KW Glycoprotein; Ion transport; Membrane; Phosphoprotein; Potassium;
KW Potassium transport; Reference proteome; Repeat; Sodium; Sodium transport;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..670
FT /note="Sodium/potassium/calcium exchanger 2"
FT /id="PRO_0000019368"
FT TOPO_DOM 1..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..133
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..205
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..506
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 507..527
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 528..542
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 543..563
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 564..578
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 579..599
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 600..611
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 612..632
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 633..639
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 640..660
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 661..670
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REPEAT 175..215
FT /note="Alpha-1"
FT REPEAT 550..581
FT /note="Alpha-2"
FT REGION 67..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 91..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 312..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 394..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 337
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 341
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 360..376
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:9461611"
FT /id="VSP_006165"
FT CONFLICT 309
FT /note="Q -> R (in Ref. 1; AAC19404)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 670 AA; 74657 MW; 4E855A467FC6679E CRC64;
MDLHQSATVR LLQEWCSHES PSGCRRHYNT RKKLKLIRVI GLVMGLVAVS TVPFSISAFT
ETYSQNNRGE ASDVTGPRAA PGHRQRTLLD LNDKIRDYTP QPPASQEDRS ENGTDHAQGD
YPKDVFSLEE RRKGAIILHV IGMIYMFIAL AIVCDEFFVP SLTVITEKLG ISDDVAGATF
MAAGGSAPEL FTSLIGVFIA HSNVGIGTIV GSAVFNILFV IGMCALFSRE ILNLTWWPLF
RDVSFYIVDL IMLIIFFLDN VIMWWESLLL LTAYFAYVVF MKFNVQVERW VKQMINRNKV
VKVTVSEAQA KASTAGDKEE PTLPNKPRLQ RGGSSASLHN SLMRNSIFQL MIHTLDPLAE
ELGSYGKLKY YDTMTEEGRF REKASILHKI AKKKCQVDEN ERQNGAANHV DYAAEKIELP
NSTSTEVEMT PSSEASEPVQ NGNLSHSIEA ADAPQATETA EEDDDQPLSL SWPSNTRKQI
TFLIVLPIVF PLWITLPDVR KPASKKFFPI TFFGSITWIA VFSYLMVWWA HQVGETIGIS
EEIMGLTILA AGTSIPDLIT SVIVARKGLG DMAVSSSVGS NIFDITVGLP LPWLLYTIIH
RFKPVTVSSN GLFCAIVLLF IMLIFVILSI ALCKWRMNKI LGFIMFGLYF AFLVVSVLLE
DKVLECPVSI
