NCKP1_DANRE
ID NCKP1_DANRE Reviewed; 1128 AA.
AC B0S6R1; B0S6R0;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Nck-associated protein 1;
DE Short=NAP 1;
GN Name=nckap1; ORFNames=si:dkey-234n3.1, wu:fd05c01;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING
RP (ISOFORMS 1 AND 2).
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
CC -!- FUNCTION: Part of the WAVE complex that regulates lamellipodia
CC formation. The WAVE complex regulates actin filament reorganization via
CC its interaction with the Arp2/3 complex. Actin remodeling activity is
CC regulated by RAC1 (By similarity). Plays a role in neural tube closure.
CC {ECO:0000250, ECO:0000250|UniProtKB:Q640K3}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cell projection,
CC lamellipodium membrane {ECO:0000250}; Single-pass membrane protein
CC {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=At the interface
CC between the lamellipodial actin meshwork and the membrane.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B0S6R1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B0S6R1-2; Sequence=VSP_038573;
CC -!- SIMILARITY: Belongs to the HEM-1/HEM-2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAQ13346.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAQ14913.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; CR513785; CAQ13346.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BX548025; CAQ13346.1; JOINED; Genomic_DNA.
DR EMBL; CR513785; CAQ13347.1; -; Genomic_DNA.
DR EMBL; BX548025; CAQ13347.1; JOINED; Genomic_DNA.
DR EMBL; BX548025; CAQ14913.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CR513785; CAQ14913.1; JOINED; Genomic_DNA.
DR EMBL; BX548025; CAQ14914.1; -; Genomic_DNA.
DR EMBL; CR513785; CAQ14914.1; JOINED; Genomic_DNA.
DR RefSeq; XP_005167980.1; XM_005167923.3. [B0S6R1-1]
DR RefSeq; XP_005167981.1; XM_005167924.3. [B0S6R1-2]
DR AlphaFoldDB; B0S6R1; -.
DR SMR; B0S6R1; -.
DR STRING; 7955.ENSDARP00000116328; -.
DR PaxDb; B0S6R1; -.
DR PeptideAtlas; B0S6R1; -.
DR PRIDE; B0S6R1; -.
DR Ensembl; ENSDART00000132696; ENSDARP00000116328; ENSDARG00000060853. [B0S6R1-1]
DR GeneID; 561894; -.
DR CTD; 10787; -.
DR ZFIN; ZDB-GENE-030131-4426; nckap1.
DR eggNOG; KOG1917; Eukaryota.
DR GeneTree; ENSGT00390000016619; -.
DR InParanoid; B0S6R1; -.
DR OMA; XSIVGMT; -.
DR OrthoDB; 138196at2759; -.
DR PhylomeDB; B0S6R1; -.
DR TreeFam; TF313683; -.
DR Reactome; R-DRE-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR Reactome; R-DRE-4420097; VEGFA-VEGFR2 Pathway.
DR Reactome; R-DRE-5663213; RHO GTPases Activate WASPs and WAVEs.
DR Reactome; R-DRE-9013149; RAC1 GTPase cycle.
DR Reactome; R-DRE-9013404; RAC2 GTPase cycle.
DR Reactome; R-DRE-9013423; RAC3 GTPase cycle.
DR PRO; PR:B0S6R1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 9.
DR Bgee; ENSDARG00000060853; Expressed in early embryo and 27 other tissues.
DR ExpressionAtlas; B0S6R1; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031209; C:SCAR complex; IBA:GO_Central.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0000902; P:cell morphogenesis; IBA:GO_Central.
DR GO; GO:0030031; P:cell projection assembly; IBA:GO_Central.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0048812; P:neuron projection morphogenesis; IMP:ZFIN.
DR InterPro; IPR019137; Nck-associated_protein-1.
DR PANTHER; PTHR12093; PTHR12093; 1.
DR Pfam; PF09735; Nckap1; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Cell membrane; Cell projection; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1128
FT /note="Nck-associated protein 1"
FT /id="PRO_0000364439"
FT TRANSMEM 995..1015
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 640..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 647..665
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 74..75
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038573"
SQ SEQUENCE 1128 AA; 128741 MW; 14DE4236F8791D63 CRC64;
MSRGAMQPSQ QKLAEKLTIL NDRGIGMLTR IYNIKKACGD PKAKPSYLID KNLESAVKFI
VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCEL LNTIDACQVF
FDITVNFDLT KNYLDLVVTY TTLMLLLSRI EERKAIIGLY NYAHEMTHGS SDREYPRLGQ
MIVDYENPLK KMMEEFVPHG KSLSDALVSL QMVYPRRNLS ADQWRNAQLL SLISAPSTML
NPAQSDTMPC EYLSLDTMEK WIVFGFILCH AALNSDPAAL SLWKLALQSS TCLCLFRDEV
FHIHKAAEDL FVNIRGYNKR VNDIRECKES ALSHAGSMHR ERRKFLRSAL KELATVLADQ
PGLLGPKALF VFMALSFARD EIIWLLRHAD NIQKKSTDDF IDKHIAELIF YMEELRAHVR
KYGPVMQRYY VQYLSGFDAV VLNELVQNLS VCPEDESIIM SSFVNTMTSL SVKQVEDGEV
FDFRGMRLDW FRLQAYTSVS KASLGLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS
IFCFYSRAFE KMFQQCLELP SQSRYSISFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN
MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG KKGEPEREKP
GVESMRKNRL LVTNLDKLHT ALSELCFSIN YVPNMMVWEH TFTPREYLTS HLEIRFTKSI
VGMTMYNQTT QEIAKPSELL TSVRAYMTVL QSIENYVQID ITRVFNNVLL QQTQHLDSHG
EPTITSLYTN WYLETLLRQV SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS
ELLGPYGMKF LSESLMWHIS SQVAELKKLV VDNVEVLTQM RTSFDKPDHM AALFKRLTSV
DSVLKRMTII GVILSFRSLA QEALRDVLSC HIPFLVSSVE DFKDHIPRET DMKVAMNVYE
LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM VFVAVSMPTL ASNVMSQYSP
AIEGHCNNIH CLAKAINQIA AALFTIHKGS IEDRLKEFLA LASSSLLKIG QETDKTTTRN
RESVYLLLDM IVQESPFLTM DLLESCFPYV LLRNAYHAVY KQSVSSSA
