NCKP1_ARATH
ID NCKP1_ARATH Reviewed; 1425 AA.
AC Q5S2C4; O82180; Q66GU3; Q6BDH4;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Protein NAP1;
DE AltName: Full=ARP2/3 regulatory protein subunit NAPP;
DE AltName: Full=NAP of plants;
DE AltName: Full=Nck-associated protein 1;
DE Short=AtNAP1;
DE Short=AtNAP125;
DE AltName: Full=Protein GNARLED;
DE AltName: Full=p125Nap1;
GN Name=NAP1; Synonyms=GRL, NAP, NAPP; OrderedLocusNames=At2g35110;
GN ORFNames=T4C15.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=15516496; DOI=10.1104/pp.104.053173;
RA Li Y., Sorefan K., Hemmann G., Bevan M.W.;
RT "Arabidopsis NAP and PIR regulate actin-based cell morphogenesis and
RT multiple developmental processes.";
RL Plant Physiol. 136:3616-3627(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), IDENTIFICATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=15316111; DOI=10.1105/tpc.104.023739;
RA Brembu T., Winge P., Seem M., Bones A.M.;
RT "NAPP and PIRP encode subunits of a putative wave regulatory protein
RT complex involved in plant cell morphogenesis.";
RL Plant Cell 16:2335-2349(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-96, INTERACTION WITH PIR, AND TISSUE
RP SPECIFICITY.
RX PubMed=15296760; DOI=10.1016/j.cub.2004.06.062;
RA El-Din El-Assal S., Le J., Basu D., Mallery E.L., Szymanski D.B.;
RT "Arabidopsis GNARLED encodes a NAP125 homolog that positively regulates
RT ARP2/3.";
RL Curr. Biol. 14:1405-1409(2004).
RN [6]
RP INTERACTION WITH PIR, AND TISSUE SPECIFICITY.
RX PubMed=15294869; DOI=10.1242/dev.01307;
RA Basu D., El-Din El-Assal S., Le J., Mallery E.L., Szymanski D.B.;
RT "Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex
RT subunit SRA1 during leaf epidermal development.";
RL Development 131:4345-4355(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
CC -!- FUNCTION: Involved in regulation of actin and microtubule organization.
CC Part of a WAVE complex that activates the Arp2/3 complex.
CC -!- SUBUNIT: Binds PIR.
CC -!- INTERACTION:
CC Q5S2C4; Q5S2C3: PIR; NbExp=5; IntAct=EBI-1547830, EBI-1547858;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5S2C4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5S2C4-2; Sequence=VSP_017078;
CC -!- TISSUE SPECIFICITY: Expressed in roots, root hairs, hypocotyls,
CC cotyledons, stems, leaves, trichomes, and flowers.
CC {ECO:0000269|PubMed:15294869, ECO:0000269|PubMed:15296760,
CC ECO:0000269|PubMed:15316111, ECO:0000269|PubMed:15516496}.
CC -!- SIMILARITY: Belongs to the HEM-1/HEM-2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC61824.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY787211; AAV64872.1; -; mRNA.
DR EMBL; AY496700; AAS78643.1; -; mRNA.
DR EMBL; AC004667; AAC61824.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09065.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09066.1; -; Genomic_DNA.
DR EMBL; AY662956; AAT76307.1; -; mRNA.
DR EMBL; BK004072; DAA04563.1; -; Genomic_DNA.
DR PIR; G84764; G84764.
DR RefSeq; NP_001031485.1; NM_001036408.3. [Q5S2C4-2]
DR RefSeq; NP_181056.2; NM_129064.3. [Q5S2C4-1]
DR AlphaFoldDB; Q5S2C4; -.
DR SMR; Q5S2C4; -.
DR BioGRID; 3423; 12.
DR DIP; DIP-29825N; -.
DR IntAct; Q5S2C4; 5.
DR STRING; 3702.AT2G35110.1; -.
DR iPTMnet; Q5S2C4; -.
DR PaxDb; Q5S2C4; -.
DR PRIDE; Q5S2C4; -.
DR ProteomicsDB; 251124; -. [Q5S2C4-1]
DR EnsemblPlants; AT2G35110.1; AT2G35110.1; AT2G35110. [Q5S2C4-1]
DR EnsemblPlants; AT2G35110.2; AT2G35110.2; AT2G35110. [Q5S2C4-2]
DR GeneID; 818077; -.
DR Gramene; AT2G35110.1; AT2G35110.1; AT2G35110. [Q5S2C4-1]
DR Gramene; AT2G35110.2; AT2G35110.2; AT2G35110. [Q5S2C4-2]
DR KEGG; ath:AT2G35110; -.
DR Araport; AT2G35110; -.
DR TAIR; locus:2063479; AT2G35110.
DR eggNOG; KOG1917; Eukaryota.
DR InParanoid; Q5S2C4; -.
DR OMA; VGMVMYN; -.
DR OrthoDB; 138196at2759; -.
DR PhylomeDB; Q5S2C4; -.
DR PRO; PR:Q5S2C4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q5S2C4; baseline and differential.
DR Genevisible; Q5S2C4; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0031209; C:SCAR complex; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; IMP:TAIR.
DR GO; GO:0045010; P:actin nucleation; TAS:TAIR.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0000902; P:cell morphogenesis; IBA:GO_Central.
DR GO; GO:0030031; P:cell projection assembly; IBA:GO_Central.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0009825; P:multidimensional cell growth; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0010090; P:trichome morphogenesis; IMP:TAIR.
DR InterPro; IPR019137; Nck-associated_protein-1.
DR PANTHER; PTHR12093; PTHR12093; 1.
DR Pfam; PF09735; Nckap1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome.
FT CHAIN 1..1425
FT /note="Protein NAP1"
FT /id="PRO_0000216178"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1299..1425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1299..1409
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 363..391
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15316111"
FT /id="VSP_017078"
FT CONFLICT 547
FT /note="Missing (in Ref. 1; AAV64872)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1425 AA; 158915 MW; A2008CEC598BB6F1 CRC64;
MANSRQYYPS QDESMSPTSV RSREWEGPSR WTEYLGPEMA ASVSSTRSSK QIDGHVGGST
KALNIQWVVQ MIEVADGLMA KMYRLNQILE YPDPVGHVFS EAFWKAGVFP NHPRICTLLS
KKFPEHFSKL QLERIDKFSL DSLHDGAELH LQSLEPWIQL LLDLMAFREQ ALRLILDLSS
TVITLLPHQN SLILHAFMDL FCAFVRVNLF AEKIPRKMLL QVYNLLHALS RNDRDCDFYH
RLVQFIDSYD PPLKGLQEDL NFVSPRIGEV LEAVGPSIFL SADTRKLRNE GFLSPYHPRF
PDILTNSAHP MRAQDLANVT SYREWVLLGY LVCPDELLRV TSIDIALVVL KENLVVTLFR
DEVSLYQMVC EKEFGIGISF ASADSINLTM QYILLHEDYQ LYVLPRVLES KKMAKSGRTK
QKEADLEYSV AKQVEKMISE VHEQALQLCD TIHRERRILL KQEIGRMVLF FTDQPSLLAP
NIQMVFSALA LAQSEVLWYF QHAGIASSRS KAARVIPVDI DPNDPTIGFL LDGMDRLCCL
VRKYISAARG YALSYLSSSA GRIRYLMGTP GIVALDLDPT LKGLFQRIVQ HLESIPKAQG
ENVSAITCDL SDFRKDWLSI LMIVTSSRSS INIRHLEKAT VSTGKEGLLS EGNAAYNWSR
CVDELESQLS KHGSLKKLYF YHQHLTTVFR NTMFGPEGRP QHCCAWLSVA SSFPECASLI
IPEEVTKFGR DAVLYVESLI ESIMGGLEGL INILDSEGGF GALESQLLPE QAAAYLNNAS
RISAPSVKSP RVVGGFTLPG HESYPENNKS IKMLEAAIQR LTNLCSILND MEPICVINHV
FVLREYMREC ILGNFKRRFL TALQTDNDLQ RPSVLESLIR RHMGIVHLAE QHVSMDLTQG
IREILLTEAF SGPVSSLHTF EKPAEQQQTT GSAVEVVCNW YMDNIIKDVS GAGILFAPRH
KYFKSTRPVG GYFAESVTDL KELQAFVRIF GGYGVDRLDR MMKVHTAALV NCIETSLRSN
RELIEAAAAS MHSGDRVERD ASVRQIVDLD TVIGFCIEAG QALAFDDLLA EASGAVLEDN
ASLIHSMISG IVEHIPEEIP EKKEIRRIKG VANGVGVAGD HDSEWVRLIL EEVGGANDNS
WSLLPYFFAS FMTSNAWNTT GFNIETGGFS NNIHCLARCI SAVIAGSEYV RLQREYQQQH
QSLSNGHHSS ENLDSEFPPR VTAEASIKSS MLLFVKFAAS IVLDSWSEAN RSHLVAKLIF
LDQLCEISPY LPRSSLESHV PYTILRSIYT QYYSNTPSTP LSTASPYHSP SVSLIHASPS
MKNSTTPQRG SGSGSSSTAA PDSGYFKGSS SSLYGQEHYT ESETGNSRNN ENNNNNKQRG
SSRRSGPLDY SSSHKGGSGS NSTGPSPLPR FAVSRSGPIS YKQHN
