ID   NCAP_MPV15              Reviewed;         393 AA.
AC   P26589; Q5MKM8;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   23-FEB-2022, entry version 98.
DE   RecName: Full=Nucleoprotein;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=NP;
DE            Short=Protein N;
GN   Name=N; Synonyms=NP;
OS   Murine pneumonia virus (strain 15) (MPV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus.
OX   NCBI_TaxID=296738;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Non-pathogenic 15;
RX   PubMed=1848602; DOI=10.1099/0022-1317-72-3-677;
RA   Barr J., Chambers P., Pringle C.R., Easton A.J.;
RT   "Sequence of the major nucleocapsid protein gene of pneumonia virus of
RT   mice: sequence comparisons suggest structural homology between nucleocapsid
RT   proteins of pneumoviruses, paramyxoviruses, rhabdoviruses and
RT   filoviruses.";
RL   J. Gen. Virol. 72:677-685(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15604443; DOI=10.1099/vir.0.80315-0;
RA   Thorpe L.C., Easton A.J.;
RT   "Genome sequence of the non-pathogenic strain 15 of pneumonia virus of mice
RT   and comparison with the genome of the pathogenic strain J3666.";
RL   J. Gen. Virol. 86:159-169(2005).
RN   [3]
RP   INTERACTION WITH P PROTEIN.
RX   PubMed=13679601; DOI=10.1099/vir.0.19370-0;
RA   Stokes H.L., Easton A.J., Marriott A.C.;
RT   "Chimeric pneumovirus nucleocapsid (N) proteins allow identification of
RT   amino acids essential for the function of the respiratory syncytial virus N
RT   protein.";
RL   J. Gen. Virol. 84:2679-2683(2003).
CC   -!- FUNCTION: Encapsidates the viral RNA genome by forming a left-handed
CC       helical nucleocapsid that protects the RNA from nucleases. RNA
CC       replication depends on the availability of soluble nucleoprotein. The
CC       encapsidated genomic RNA is termed the NC and serves as template for
CC       transcription and replication. {ECO:0000250|UniProtKB:P03418}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Interacts with the
CC       phosphoprotein P. When in a monomeric RNA-free form, interacts with the
CC       phosphoprotein (via N-terminus). Interacts with protein M2-1; this
CC       interaction allows the association of nucleocapsid with the matrix
CC       protein. {ECO:0000250|UniProtKB:P03418}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:P03418}. Host
CC       cytoplasm {ECO:0000250|UniProtKB:P03418}. Note=Localizes in cytoplasmic
CC       inclusion bodies. {ECO:0000250|UniProtKB:P03418}.
CC   -!- SIMILARITY: Belongs to the paramyxoviruses nucleocapsid family.
CC       {ECO:0000305}.
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DR   EMBL; AY743910; AAW02834.1; -; Genomic_RNA.
DR   PIR; A38474; VHNZPM.
DR   SMR; P26589; -.
DR   Proteomes; UP000133604; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039580; P:suppression by virus of host PKR signaling; IEA:UniProtKB-KW.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR004930; Pneumo_ncap.
DR   Pfam; PF03246; Pneumo_ncap; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Helical capsid protein; Host cytoplasm;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus;
KW   Inhibition of host PKR by virus; Reference proteome; Ribonucleoprotein;
KW   RNA-binding; Viral immunoevasion; Viral nucleoprotein; Virion.
FT   CHAIN           1..393
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000142675"
FT   VARIANT         234
FT                   /note="R -> K (in strain: Non-pathogenic 15)"
SQ   SEQUENCE   393 AA;  43135 MW;  A641842509BA7D38 CRC64;
     MSLDRLKLND VSNKDSLLSN CKYSVTRSTG DVTSVSGHAM QKALARTLGM FLLTAFNRCE
     EVAEIGLQYA MSLLGRDDSI KILREAGYNV KCVDTQLKDF TIKLQGKEYK IQVLDIVGID
     AANLADLEIQ ARGVVAKELK TGARLPDNRR HDAPDCGVIV LCIAALVVSK LAAGDRGGLD
     AVERRALNVL KAEKARYPNM EVKQIAESFY DLFERKPYYI DVFITFGLAQ SSVRGGSKVE
     GLFSGLFMNA YGAGQVMLRW GLLAKSVKNI MLGHASVQAE MEQVVEVYEY AQKQGGEAGF
     YHIRNNPKAS LLSLTNCPNF TSVVLGNAAG LGIIGSYKGA PRNRELFDAA KDYAERLKDN
     NVINYSALNL TAEERELISQ QLNIVDDTPD DDI