ID   NCAP_MOKV               Reviewed;         450 AA.
AC   P0C570;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   23-FEB-2022, entry version 61.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   Mokola virus (MOKV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC   Lyssavirus.
OX   NCBI_TaxID=12538;
OH   NCBI_TaxID=9615; Canis lupus familiaris (Dog) (Canis familiaris).
OH   NCBI_TaxID=269271; Chodsigoa caovansunga (Van Sung's shrew).
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9989; Rodentia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9225031; DOI=10.1099/0022-1317-78-7-1571;
RA   Le Mercier P., Jacob Y., Tordo N.;
RT   "The complete Mokola virus genome sequence: structure of the RNA-dependent
RT   RNA polymerase.";
RL   J. Gen. Virol. 78:1571-1576(1997).
CC   -!- FUNCTION: Encapsidates the genome in a ratio of one protein N per nine
CC       ribonucleotides, protecting it from nucleases. If expressed without
CC       protein P it binds non-specifically RNA and therefore can bind it's own
CC       mRNA. Interaction with protein P abolishes any non-specific RNA
CC       binding, and prevents phosphorylation. The soluble N-P complex
CC       encapsidates specifically the genomic RNA, with protein N protecting
CC       the genome like a pearl necklace. The encapsidated genomic RNA is
CC       termed the nucleocapsid (NC) and serves as template for viral
CC       transcription and replication. Protein N binds protein P in the NC
CC       through a different interaction, and can be phosphorylated. Subsequent
CC       viral replication is dependent on intracellular concentration of newly
CC       synthesized protein N. During replication, encapsidation by protein N
CC       is coupled to RNA synthesis and all replicative products are resistant
CC       to nucleases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. In nucleocapsid, binds protein P and thereby positions the
CC       polymerase on the template. Protein P acts as a chaperone on free
CC       protein N to prevent it from aggregation before encapsidating genomic
CC       RNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC   -!- PTM: Phosphorylated by host CK2. Unphosphorylated protein N seems to
CC       have a better affinity for leader viral promoter encapsidation.
CC       Phosphorylation of protein N in ribonucleocapsid may stabilize the
CC       interaction with protein P, thereby playing an important role in viral
CC       transcription/replication (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Displays a superantigen activity in human and mouse,
CC       activating mostly V-beta-8 subtypes of T-cell receptor. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lyssavirus nucleocapsid protein family.
CC       {ECO:0000305}.
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DR   EMBL; Y09762; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   RefSeq; YP_142350.1; NC_006429.1.
DR   SMR; P0C570; -.
DR   PRIDE; P0C570; -.
DR   GeneID; 3159476; -.
DR   KEGG; vg:3159476; -.
DR   Proteomes; UP000006826; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3570.10; -; 1.
DR   Gene3D; 1.10.3610.10; -; 1.
DR   InterPro; IPR000448; Rhabdo_ncapsid.
DR   InterPro; IPR023331; Rhabdovirus_ncapsid_C.
DR   InterPro; IPR023330; Rhabdovirus_ncapsid_N.
DR   InterPro; IPR035961; Rhabdovirus_nucleoprotein-like.
DR   Pfam; PF00945; Rhabdo_ncap; 1.
DR   SUPFAM; SSF140809; SSF140809; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW   Reference proteome; Ribonucleoprotein; RNA-binding; Superantigen;
KW   Viral nucleoprotein; Virion.
FT   CHAIN           1..450
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000295205"
FT   MOD_RES         389
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   450 AA;  50457 MW;  3AB2B85C0D1B1AC1 CRC64;
     MESDKIVFKV NNQVVSLKPE VISDQYEYKY PAILDGKKPG ITLGKAPDLN TAYKSILSGM
     KAAKLDPDDV CSYLAAAMHL FEGVCPEDWV SYGIVIAKKG EKINPSVIVD IVRTNVEGNW
     AQAGGTDVIR DPTMAEHASL VGLLLCLYRL SKIVGQNTAN YKTNVADRME QIFETAPFAK
     VVEHHTLMTT HKMCANWSTI PNFRFLVGTY DMFFARVEHI YSALRVGTVV TAYEDCSGLV
     SFTGFIKQIN LSPRDALLYF FHKNFEGEIK RMFEPGQETA VPHSYFIHFR ALGLSGKSPY
     SSNAVGHTFN LIHFVGCYMG QIRSLNATVI QTCAPHEMSV LGGYLGEEFF GKGTFERRFF
     RDEKEMQDYT ELEEARVEAS LADDGTVDSD EEDFFSGETR SPEAVYSRIM MNNGKLKKVH
     IRRYIAVSSN HQARPNSFAE FLNKVYADGS