NAC35_ARATH
ID NAC35_ARATH Reviewed; 414 AA.
AC Q9ZVP8; Q8H131; Q9C5E2;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=NAC domain-containing protein 35 {ECO:0000303|PubMed:15029955};
DE Short=ANAC035 {ECO:0000303|PubMed:15029955};
DE AltName: Full=Protein LONG VEGETATIVE PHASE 1 {ECO:0000303|PubMed:17653269};
DE Short=AtLOV1 {ECO:0000303|PubMed:17653269};
GN Name=NAC035 {ECO:0000312|EMBL:AEC05581.1};
GN OrderedLocusNames=At2g02450 {ECO:0000312|Araport:AT2G02450};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=17653269; DOI=10.1371/journal.pone.0000642;
RA Yoo S.Y., Kim Y., Kim S.Y., Lee J.S., Ahn J.H.;
RT "Control of flowering time and cold response by a NAC-domain protein in
RT Arabidopsis.";
RL PLoS ONE 2:E642-E642(2007).
CC -!- FUNCTION: Transcription factor that acts as a floral repressor.
CC Controls flowering time by negatively regulating CONSTANS (CO)
CC expression in a GIGANTEA (GI)-independent manner. Regulates the plant
CC cold response by positive regulation of the cold response genes COR15A
CC and KIN1. May coordinate cold response and flowering time.
CC {ECO:0000269|PubMed:17653269}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:17653269}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZVP8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZVP8-2; Sequence=VSP_058389;
CC -!- TISSUE SPECIFICITY: Expressed in aerial organs in early stages of
CC seedling development. {ECO:0000269|PubMed:17653269}.
CC -!- INDUCTION: Circadian regulation with a peak of expression at dawn under
CC continuous light conditions (PubMed:17653269). Circadian regulation
CC with a peak of expression around dusk and lowest expression around dawn
CC under continuous light conditions (at protein level) (PubMed:17653269).
CC {ECO:0000269|PubMed:17653269}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- DISRUPTION PHENOTYPE: Early flowering phenotype under long-day
CC conditions. Hypersensitivity to cold. {ECO:0000269|PubMed:17653269}.
CC -!- MISCELLANEOUS: The gain-of-function mutant lov1-1D (T-DNA tagging)
CC shows a late-flowering phenotype under long-day conditions, and
CC freezing tolerance. {ECO:0000269|PubMed:17653269}.
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DR EMBL; AC005312; AAC78526.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05581.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05582.1; -; Genomic_DNA.
DR EMBL; AF360308; AAK26018.2; -; mRNA.
DR EMBL; BT000874; AAN41274.1; -; mRNA.
DR PIR; G84436; G84436.
DR PIR; H84436; H84436.
DR RefSeq; NP_565284.3; NM_126301.4. [Q9ZVP8-1]
DR RefSeq; NP_850986.1; NM_180655.2. [Q9ZVP8-2]
DR AlphaFoldDB; Q9ZVP8; -.
DR SMR; Q9ZVP8; -.
DR IntAct; Q9ZVP8; 27.
DR STRING; 3702.AT2G02450.2; -.
DR PaxDb; Q9ZVP8; -.
DR PRIDE; Q9ZVP8; -.
DR ProteomicsDB; 251277; -. [Q9ZVP8-1]
DR EnsemblPlants; AT2G02450.1; AT2G02450.1; AT2G02450. [Q9ZVP8-2]
DR EnsemblPlants; AT2G02450.2; AT2G02450.2; AT2G02450. [Q9ZVP8-1]
DR GeneID; 814775; -.
DR Gramene; AT2G02450.1; AT2G02450.1; AT2G02450. [Q9ZVP8-2]
DR Gramene; AT2G02450.2; AT2G02450.2; AT2G02450. [Q9ZVP8-1]
DR KEGG; ath:AT2G02450; -.
DR Araport; AT2G02450; -.
DR TAIR; locus:2056266; AT2G02450.
DR eggNOG; ENOG502QVXP; Eukaryota.
DR InParanoid; Q9ZVP8; -.
DR OMA; HYINIPF; -.
DR OrthoDB; 1067128at2759; -.
DR PhylomeDB; Q9ZVP8; -.
DR PRO; PR:Q9ZVP8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZVP8; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Flowering; Nucleus; Reference proteome;
KW Repressor; Stress response; Transcription; Transcription regulation.
FT CHAIN 1..414
FT /note="NAC domain-containing protein 35"
FT /id="PRO_0000436556"
FT DOMAIN 51..198
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 149..204
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..47
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..251
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 331..365
FT /note="Missing (in isoform 2)"
FT /id="VSP_058389"
SQ SEQUENCE 414 AA; 47303 MW; 2D04A80EBB0F21E9 CRC64;
MAIVSSTTSI IPMSNQVNNN EKGIEDNDHR GGQESHVQNE DEADDHDHDM VMPGFRFHPT
EEELIEFYLR RKVEGKRFNV ELITFLDLYR YDPWELPAMA AIGEKEWYFY VPRDRKYRNG
DRPNRVTTSG YWKATGADRM IRSETSRPIG LKKTLVFYSG KAPKGTRTSW IMNEYRLPHH
ETEKYQKAEI SLCRVYKRPG VEDHPSVPRS LSTRHHNHNS STSSRLALRQ QQHHSSSSNH
SDNNLNNNNN INNLEKLSTE YSGDGSTTTT TTNSNSDVTI ALANQNIYRP MPYDTSNNTL
IVSTRNHQDD DETAIVDDLQ RLVNYQISDG GNINHQYFQI AQQFHHTQQQ NANANALQLV
AAATTATTLM PQTQAALAMN MIPAGTIPNN ALWDMWNPIV PDGNRDHYTN IPFK
