NAC22_ARATH
ID NAC22_ARATH Reviewed; 324 AA.
AC Q84TE6; Q0WRG2; Q9SE10; Q9SGS9;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=NAC domain-containing protein 21/22;
DE Short=ANAC021/ANAC022;
GN Name=NAC021; Synonyms=NAC022, NAC1; OrderedLocusNames=At1g56010;
GN ORFNames=F14J16.32, T6H22.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP INDUCTION (ISOFORM 1).
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11114891; DOI=10.1101/gad.852200;
RA Xie Q., Frugis G., Colgan D.F., Chua N.-H.;
RT "Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote
RT lateral root development.";
RL Genes Dev. 14:3024-3036(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH SINAT5, UBIQUITINATION, AND DEGRADATION.
RX PubMed=12226665; DOI=10.1038/nature00998;
RA Xie Q., Guo H.-S., Dallman G., Fang S., Weissman A.M., Chua N.-H.;
RT "SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin
RT signals.";
RL Nature 419:167-170(2002).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
CC -!- FUNCTION: Transcriptional activator that mediates auxin signaling to
CC promote lateral root development. Activates the expression of two
CC downstream auxin-responsive genes, DBP and AIR3.
CC {ECO:0000269|PubMed:11114891}.
CC -!- SUBUNIT: Dimer. Interacts with SINAT5. {ECO:0000269|PubMed:12226665}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=ANAC022;
CC IsoId=Q84TE6-1; Sequence=Displayed;
CC Name=2; Synonyms=ANAC021;
CC IsoId=Q84TE6-2; Sequence=VSP_011189;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the root tip and in
CC lateral root initiation sites. Also detected in expanding cotyledon,
CC and in leaf primordia. {ECO:0000269|PubMed:11114891}.
CC -!- INDUCTION: Induced by auxin.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain.
CC -!- PTM: Ubiquitinated (Probable). The interaction with SINAT5 mediate its
CC proteasome-dependent degradation (PubMed:12226665).
CC {ECO:0000269|PubMed:12226665, ECO:0000305|PubMed:12226665}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF02847.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF198054; AAF21437.1; -; mRNA.
DR EMBL; AC002304; AAF79328.1; -; Genomic_DNA.
DR EMBL; AC009894; AAF02847.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33330.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33331.1; -; Genomic_DNA.
DR EMBL; BT005873; AAO64808.1; -; mRNA.
DR EMBL; AK228347; BAF00287.1; -; mRNA.
DR EMBL; AY085996; AAM63206.1; -; mRNA.
DR PIR; C96601; C96601.
DR RefSeq; NP_175997.1; NM_104479.2. [Q84TE6-1]
DR RefSeq; NP_849817.1; NM_179486.2. [Q84TE6-2]
DR AlphaFoldDB; Q84TE6; -.
DR SMR; Q84TE6; -.
DR BioGRID; 27277; 5.
DR IntAct; Q84TE6; 2.
DR STRING; 3702.AT1G56010.2; -.
DR PaxDb; Q84TE6; -.
DR PRIDE; Q84TE6; -.
DR EnsemblPlants; AT1G56010.1; AT1G56010.1; AT1G56010. [Q84TE6-2]
DR EnsemblPlants; AT1G56010.2; AT1G56010.2; AT1G56010. [Q84TE6-1]
DR GeneID; 842052; -.
DR Gramene; AT1G56010.1; AT1G56010.1; AT1G56010. [Q84TE6-2]
DR Gramene; AT1G56010.2; AT1G56010.2; AT1G56010. [Q84TE6-1]
DR KEGG; ath:AT1G56010; -.
DR Araport; AT1G56010; -.
DR TAIR; locus:2012035; AT1G56010.
DR eggNOG; ENOG502QRZB; Eukaryota.
DR HOGENOM; CLU_035664_5_1_1; -.
DR InParanoid; Q84TE6; -.
DR OMA; DIPKMAC; -.
DR PhylomeDB; Q84TE6; -.
DR PRO; PR:Q84TE6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84TE6; baseline and differential.
DR Genevisible; Q84TE6; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; TAS:TAIR.
DR GO; GO:0048527; P:lateral root development; TAS:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..324
FT /note="NAC domain-containing protein 21/22"
FT /id="PRO_0000132310"
FT DOMAIN 19..171
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT MOTIF 120..137
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..67
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.5"
FT /id="VSP_011189"
SQ SEQUENCE 324 AA; 36569 MW; C70ED705D1A06957 CRC64;
METEEEMKES SISMVEAKLP PGFRFHPKDD ELVCDYLMRR SLHNNHRPPL VLIQVDLNKC
EPWDIPKMAC VGGKDWYFYS QRDRKYATGL RTNRATATGY WKATGKDRTI LRKGKLVGMR
KTLVFYQGRA PRGRKTDWVM HEFRLQGSHH PPNHSLSSPK EDWVLCRVFH KNTEGVICRD
NMGSCFDETA SASLPPLMDP YINFDQEPSS YLSDDHHYII NEHVPCFSNL SQNQTLNSNL
TNSVSELKIP CKNPNPLFTG GSASATLTGL DSFCSSDQMV LRALLSQLTK IDGSLGPKES
QSYGEGSSES LLTDIGIPST VWNC
