ID   NA24_STIHA              Reviewed;          84 AA.
AC   B1B5I9;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Delta-stichotoxin-Shd3a {ECO:0000303|PubMed:22683676};
DE            Short=Delta-SHTX-Shd3a {ECO:0000303|PubMed:22683676};
DE   AltName: Full=Sodium channel toxin SHTX IV {ECO:0000303|PubMed:18243416};
DE   AltName: Full=Sodium channel toxin SHTX-4;
DE   Flags: Precursor;
OS   Stichodactyla haddoni (Saddle carpet anemone) (Haddon's sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Stichodactylidae; Stichodactyla.
OX   NCBI_TaxID=475174;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 36-79, AMIDATION AT LYS-83,
RP   MASS SPECTROMETRY, AND TOXIC DOSE.
RX   PubMed=18243416; DOI=10.1016/j.peptides.2007.12.010;
RA   Honma T., Kawahata S., Ishida M., Nagai H., Nagashima Y., Shiomi K.;
RT   "Novel peptide toxins from the sea anemone Stichodactyla haddoni.";
RL   Peptides 29:536-544(2008).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA   Oliveira J.S., Fuentes-Silva D., King G.F.;
RT   "Development of a rational nomenclature for naming peptide and protein
RT   toxins from sea anemones.";
RL   Toxicon 60:539-550(2012).
CC   -!- FUNCTION: Binds specifically to voltage-gated sodium channels (Nav),
CC       thereby delaying their inactivation during signal transduction.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted. Nematocyst.
CC   -!- MASS SPECTROMETRY: Mass=5229.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:18243416};
CC   -!- TOXIC DOSE: LD(50) is 93 ug/kg into crabs.
CC       {ECO:0000269|PubMed:18243416}.
CC   -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC       family. Type II subfamily. {ECO:0000305}.
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DR   EMBL; AB362570; BAG12825.1; -; mRNA.
DR   AlphaFoldDB; B1B5I9; -.
DR   SMR; B1B5I9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Nematocyst; Neurotoxin;
KW   Secreted; Signal; Toxin; Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..33
FT                   /evidence="ECO:0000269|PubMed:18243416"
FT                   /id="PRO_0000344517"
FT   CHAIN           36..83
FT                   /note="Delta-stichotoxin-Shd3a"
FT                   /id="PRO_0000344518"
FT   MOD_RES         83
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000305|PubMed:18243416"
FT   DISULFID        38..78
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        40..68
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        61..79
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
SQ   SEQUENCE   84 AA;  9164 MW;  832FBD6FD5C0ECB9 CRC64;
     MAYLKIVLVA LMLVLGVSAM RLSDQEDQDV SVVKRAACKC DDDGPDIRSA TLTGTVDFWN
     CNEGWEKCTA VYTAVASCCR KKKG