ID   A1245_CONAA             Reviewed;          11 AA.
AC   P0DUU4;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=Conotoxin Ama1245 {ECO:0000303|PubMed:31203793};
DE   Contains:
DE     RecName: Full=Conotoxin Ama1158 {ECO:0000303|PubMed:31203793};
OS   Conus amadis (Amadis cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Leptoconus.
OX   NCBI_TaxID=198732;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, HYDROXYLATION AT
RP   PRO-10, AND AMIDATION AT CYS-11.
RC   TISSUE=Venom;
RX   PubMed=31203793; DOI=10.2174/0929866526666190614144006;
RA   Rajesh R.P., Franklin J.B., Badsha I., Arjun P., Jain R.P., Vignesh M.S.,
RA   Kannan R.R.;
RT   "Proteome based de novo sequencing of novel conotoxins from marine
RT   molluscivorous cone snail Conus amadis and neurological activities of its
RT   natural venom in zebrafish model.";
RL   Protein Pept. Lett. 26:819-833(2019).
CC   -!- FUNCTION: Probable toxin that inhibits ion channels. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31203793}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:31203793}.
CC   -!- DOMAIN: The cysteine framework is I (CC-C-C). Alpha3/2 pattern.
CC   -!- PTM: Contains 2 disulfide bonds. {ECO:0000269|PubMed:31203793}.
CC   -!- MASS SPECTROMETRY: [Conotoxin Ama1158]: Mass=1158.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:31203793};
CC   -!- MASS SPECTROMETRY: [Conotoxin Ama1245]: Mass=1245.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:31203793};
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Hydroxylation;
KW   Ion channel impairing toxin; Secreted; Toxin.
FT   PEPTIDE         1..11
FT                   /note="Conotoxin Ama1245"
FT                   /evidence="ECO:0000269|PubMed:31203793"
FT                   /id="PRO_0000453583"
FT   PEPTIDE         2..11
FT                   /note="Conotoxin Ama1158"
FT                   /evidence="ECO:0000269|PubMed:31203793"
FT                   /id="PRO_0000453584"
FT   MOD_RES         10
FT                   /note="4-hydroxyproline; in Ama1158 and Ama1245"
FT                   /evidence="ECO:0000269|PubMed:31203793"
FT   MOD_RES         11
FT                   /note="Cysteine amide; in Ama1158 and Ama1245"
FT                   /evidence="ECO:0000269|PubMed:31203793"
FT   UNSURE          2
FT                   /note="Q or K"
FT                   /evidence="ECO:0000305|PubMed:31203793"
FT   UNSURE          6
FT                   /note="Q or K"
FT                   /evidence="ECO:0000305|PubMed:31203793"
FT   UNSURE          7
FT                   /note="I or L"
FT                   /evidence="ECO:0000305|PubMed:31203793"
SQ   SEQUENCE   11 AA;  1234 MW;  936C6661B046CB5E CRC64;
     SQCCYQICSP C