MTR1L_HUMAN
ID MTR1L_HUMAN Reviewed; 617 AA.
AC Q13585; Q0VGG3; Q3ZAR0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 3.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Melatonin-related receptor;
DE AltName: Full=G protein-coupled receptor 50;
DE AltName: Full=H9;
GN Name=GPR50;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS 502-THR--HIS-505 DEL; ALA-532 AND
RP VAL-606.
RC TISSUE=Pituitary;
RX PubMed=8647286; DOI=10.1016/0014-5793(96)00437-1;
RA Reppert S.M., Weaver D.R., Ebisawa T., Mahle C.D., Kolakowski L.F. Jr.;
RT "Cloning of a melatonin-related receptor from human pituitary.";
RL FEBS Lett. 386:219-224(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS 502-THR--HIS-505 DEL AND ALA-532.
RC TISSUE=Pituitary;
RA Kaighin V.A., Martin A.L., Aronstam R.S.;
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS 502-THR--HIS-505 DEL;
RP ALA-532 AND VAL-606.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBUNIT.
RX PubMed=16778767; DOI=10.1038/sj.emboj.7601193;
RA Levoye A., Dam J., Ayoub M.A., Guillaume J.L., Couturier C., Delagrange P.,
RA Jockers R.;
RT "The orphan GPR50 receptor specifically inhibits MT1 melatonin receptor
RT function through heterodimerization.";
RL EMBO J. 25:3012-3023(2006).
RN [5]
RP VARIANTS 502THR--HIS505 DEL; ALA-528 AND ILE-606.
RX PubMed=16436372; DOI=10.1194/jlr.m500338-jlr200;
RA Bhattacharyya S., Luan J., Challis B., Keogh J., Montague C., Brennand J.,
RA Morten J., Lowenbeim S., Jenkins S., Farooqi I.S., Wareham N.J.,
RA O'Rahilly S.;
RT "Sequence variants in the melatonin-related receptor gene (GPR50) associate
RT with circulating triglyceride and HDL levels.";
RL J. Lipid Res. 47:761-766(2006).
CC -!- FUNCTION: Does not bind melatonin.
CC -!- SUBUNIT: Homodimer, and heterodimer with MTNR1A and MTNR1B.
CC {ECO:0000269|PubMed:16778767}.
CC -!- INTERACTION:
CC Q13585; P04731: MT1A; NbExp=3; IntAct=EBI-8550965, EBI-8045030;
CC Q13585; Q7Z3I7: ZNF572; NbExp=3; IntAct=EBI-8550965, EBI-10172590;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Hypothalamus and pituitary.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U52219; AAC50614.1; -; mRNA.
DR EMBL; EU432118; ABY87917.1; -; mRNA.
DR EMBL; BC103696; AAI03697.1; -; mRNA.
DR EMBL; BC105683; AAI05684.1; -; mRNA.
DR EMBL; BC105684; AAI05685.1; -; mRNA.
DR CCDS; CCDS44012.1; -.
DR PIR; S70520; S70520.
DR RefSeq; NP_004215.2; NM_004224.3.
DR AlphaFoldDB; Q13585; -.
DR SMR; Q13585; -.
DR BioGRID; 114674; 57.
DR IntAct; Q13585; 23.
DR MINT; Q13585; -.
DR STRING; 9606.ENSP00000218316; -.
DR ChEMBL; CHEMBL3341577; -.
DR iPTMnet; Q13585; -.
DR PhosphoSitePlus; Q13585; -.
DR BioMuta; GPR50; -.
DR DMDM; 296439298; -.
DR EPD; Q13585; -.
DR jPOST; Q13585; -.
DR MassIVE; Q13585; -.
DR MaxQB; Q13585; -.
DR PaxDb; Q13585; -.
DR PeptideAtlas; Q13585; -.
DR PRIDE; Q13585; -.
DR ProteomicsDB; 59583; -.
DR Antibodypedia; 7082; 305 antibodies from 34 providers.
DR DNASU; 9248; -.
DR Ensembl; ENST00000218316.4; ENSP00000218316.3; ENSG00000102195.10.
DR GeneID; 9248; -.
DR KEGG; hsa:9248; -.
DR MANE-Select; ENST00000218316.4; ENSP00000218316.3; NM_004224.3; NP_004215.2.
DR UCSC; uc010ntg.3; human.
DR CTD; 9248; -.
DR DisGeNET; 9248; -.
DR GeneCards; GPR50; -.
DR HGNC; HGNC:4506; GPR50.
DR HPA; ENSG00000102195; Tissue enriched (pituitary).
DR MIM; 300207; gene.
DR neXtProt; NX_Q13585; -.
DR OpenTargets; ENSG00000102195; -.
DR PharmGKB; PA28895; -.
DR VEuPathDB; HostDB:ENSG00000102195; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000160515; -.
DR HOGENOM; CLU_468199_0_0_1; -.
DR InParanoid; Q13585; -.
DR OMA; GHHKSVF; -.
DR OrthoDB; 907115at2759; -.
DR PhylomeDB; Q13585; -.
DR TreeFam; TF331693; -.
DR PathwayCommons; Q13585; -.
DR SignaLink; Q13585; -.
DR BioGRID-ORCS; 9248; 10 hits in 697 CRISPR screens.
DR GeneWiki; GPR50; -.
DR GenomeRNAi; 9248; -.
DR Pharos; Q13585; Tbio.
DR PRO; PR:Q13585; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q13585; protein.
DR Bgee; ENSG00000102195; Expressed in adenohypophysis and 39 other tissues.
DR Genevisible; Q13585; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0008502; F:melatonin receptor activity; IEA:InterPro.
DR GO; GO:0007267; P:cell-cell signaling; TAS:ProtInc.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR002280; Mel_rcpt_1X.
DR InterPro; IPR000025; Melatonin_rcpt.
DR PANTHER; PTHR24228:SF56; PTHR24228:SF56; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01151; MELATONIN1XR.
DR PRINTS; PR00857; MELATONINR.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..617
FT /note="Melatonin-related receptor"
FT /id="PRO_0000069878"
FT TOPO_DOM 1..30
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..64
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..188
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..239
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 261..273
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..617
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 340..438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 464..596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..355
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..409
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 476..504
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 101..178
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 502..505
FT /note="Missing (lower fasting circulating triglyceride
FT levels)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:8647286, ECO:0000269|Ref.2"
FT /id="VAR_062254"
FT VARIANT 532
FT /note="T -> A (higher fasting circulating triglyceride
FT levels; dbSNP:rs561077)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:8647286, ECO:0000269|Ref.2"
FT /id="VAR_062255"
FT VARIANT 606
FT /note="I -> V (higher fasting circulating triglyceride
FT levels; dbSNP:rs13440581)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:8647286"
FT /id="VAR_062256"
FT CONFLICT 320
FT /note="S -> P (in Ref. 1; AAC50614)"
FT /evidence="ECO:0000305"
FT CONFLICT 448
FT /note="A -> G (in Ref. 1; AAC50614)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 617 AA; 67369 MW; A0B73A7ACE1053BB CRC64;
MGPTLAVPTP YGCIGCKLPQ PEYPPALIIF MFCAMVITIV VDLIGNSMVI LAVTKNKKLR
NSGNIFVVSL SVADMLVAIY PYPLMLHAMS IGGWDLSQLQ CQMVGFITGL SVVGSIFNIV
AIAINRYCYI CHSLQYERIF SVRNTCIYLV ITWIMTVLAV LPNMYIGTIE YDPRTYTCIF
NYLNNPVFTV TIVCIHFVLP LLIVGFCYVR IWTKVLAARD PAGQNPDNQL AEVRNFLTMF
VIFLLFAVCW CPINVLTVLV AVSPKEMAGK IPNWLYLAAY FIAYFNSCLN AVIYGLLNEN
FRREYWTIFH AMRHPIIFFS GLISDIREMQ EARTLARARA HARDQAREQD RAHACPAVEE
TPMNVRNVPL PGDAAAGHPD RASGHPKPHS RSSSAYRKSA STHHKSVFSH SKAASGHLKP
VSGHSKPASG HPKSATVYPK PASVHFKADS VHFKGDSVHF KPDSVHFKPA SSNPKPITGH
HVSAGSHSKS AFSAATSHPK PTTGHIKPAT SHAEPTTADY PKPATTSHPK PTAADNPELS
ASHCPEIPAI AHPVSDDSDL PESASSPAAG PTKPAASQLE SDTIADLPDP TVVTTSTNDY
HDVVVIDVED DPDEMAV
