ID   MTO1_CAEEL              Reviewed;         638 AA.
AC   Q20680;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Protein MTO1 homolog, mitochondrial {ECO:0000250|UniProtKB:Q9Y2Z2};
DE   AltName: Full=Mitochondrial tRNA carboxymethyl-amino-methyl modification of uridine residues protein 2 {ECO:0000312|WormBase:F52H3.2};
GN   Name=mtcu-2 {ECO:0000312|WormBase:F52H3.2};
GN   ORFNames=F52H3.2 {ECO:0000312|WormBase:F52H3.2};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Involved in the 5-carboxymethylaminomethyl modification
CC       (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs.
CC       {ECO:0000250|UniProtKB:Q9Y2Z2}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000305}.
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DR   EMBL; Z66512; CAA91322.1; -; Genomic_DNA.
DR   PIR; T22518; T22518.
DR   RefSeq; NP_496169.1; NM_063768.5.
DR   AlphaFoldDB; Q20680; -.
DR   SMR; Q20680; -.
DR   STRING; 6239.F52H3.2; -.
DR   EPD; Q20680; -.
DR   PaxDb; Q20680; -.
DR   PeptideAtlas; Q20680; -.
DR   EnsemblMetazoa; F52H3.2.1; F52H3.2.1; WBGene00009944.
DR   GeneID; 174563; -.
DR   KEGG; cel:CELE_F52H3.2; -.
DR   UCSC; F52H3.2.1; c. elegans.
DR   CTD; 174563; -.
DR   WormBase; F52H3.2; CE03398; WBGene00009944; mtcu-2.
DR   eggNOG; KOG2311; Eukaryota.
DR   GeneTree; ENSGT00390000011297; -.
DR   HOGENOM; CLU_007831_2_2_1; -.
DR   InParanoid; Q20680; -.
DR   OMA; FRPGYAI; -.
DR   OrthoDB; 1437708at2759; -.
DR   PhylomeDB; Q20680; -.
DR   PRO; PR:Q20680; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00009944; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0070899; P:mitochondrial tRNA wobble uridine modification; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   FAD; Flavoprotein; Reference proteome.
FT   CHAIN           1..638
FT                   /note="Protein MTO1 homolog, mitochondrial"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000117286"
FT   BINDING         23..28
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   638 AA;  71680 MW;  30A57D68C1A104C5 CRC64;
     MLSKSTRLLR RCFQTDVDVI VIGGGHAGCE SAAAAARCGS NTVLVTQNKN TIGEMSCNPS
     FGGIGKGHLI REVDALDGLC ARICDKSAIT YQALNRAQGP AVLGLRAQID RKLYKTQMQN
     EINSTKRLEI LEGEVAELLV ENGKIVGIRM MNETVIRTKC VVITTGTFLR AQIYQGMKTW
     PAGRIGEKSS DRLSESFLKH GFELGRLRTG TPPRLMKDSI NFSKFERVAP DRTPIPFSFL
     TKNVWISYED QLPTYLGHTN DEVCRIGNEN MHENYQVASE TTSPRYCPSL ESKLLRFPKL
     HHRLFLEHEG LDSPHIYPQG MSLTFKPEVQ TQLLRAIPGL ENVEIFQPGY GVQYDFVNPK
     QLKRTLETRK VEGMFLAGQI NGTTGYEEAA AQGVVAGINA SARAQNEPGM EVSRTEGYIG
     VLIDDLTSLG TNEPYRMLTS RAEFRLYLRP DNADIRLTEL GRRHNAISDN RWAIFTETKG
     ELNNLTQRTE EMKMSMVKWK RIIPKLAATS RNDGKVLSAF DLIHRYDLDK SDLELCLKDK
     NIGEDILERL KIEGRYQMEH ERMKAKKQEI DRESATAIPD NTDFSTMRGM SLECIEKLER
     ARPRNLAAAT RISGITPEAI VVLMRHLKNP APVRSSAV