MTH2_DROME
ID MTH2_DROME Reviewed; 518 AA.
AC Q9VRN2; B5RIK3; Q8SWY8;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2003, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Probable G-protein coupled receptor Mth-like 2;
DE AltName: Full=Protein methuselah-like 2;
DE Flags: Precursor;
GN Name=mthl2; ORFNames=CG17795;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP REVIEW.
RX PubMed=10908591; DOI=10.1083/jcb.150.2.f83;
RA Brody T., Cravchik A.;
RT "Drosophila melanogaster G protein-coupled receptors.";
RL J. Cell Biol. 150:F83-F88(2000).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAO41276.2; -; Genomic_DNA.
DR EMBL; AY094944; AAM11297.1; -; mRNA.
DR EMBL; BT044127; ACH92192.1; -; mRNA.
DR RefSeq; NP_788462.2; NM_176284.3.
DR AlphaFoldDB; Q9VRN2; -.
DR SMR; Q9VRN2; -.
DR STRING; 7227.FBpp0076796; -.
DR GlyGen; Q9VRN2; 4 sites.
DR PaxDb; Q9VRN2; -.
DR PRIDE; Q9VRN2; -.
DR DNASU; 38636; -.
DR EnsemblMetazoa; FBtr0077088; FBpp0076796; FBgn0035623.
DR GeneID; 38636; -.
DR KEGG; dme:Dmel_CG17795; -.
DR CTD; 38636; -.
DR FlyBase; FBgn0035623; mthl2.
DR VEuPathDB; VectorBase:FBgn0035623; -.
DR eggNOG; KOG4193; Eukaryota.
DR GeneTree; ENSGT00940000168274; -.
DR HOGENOM; CLU_002753_3_0_1; -.
DR InParanoid; Q9VRN2; -.
DR OMA; NAEIADC; -.
DR OrthoDB; 1153592at2759; -.
DR PhylomeDB; Q9VRN2; -.
DR BioGRID-ORCS; 38636; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38636; -.
DR PRO; PR:Q9VRN2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035623; Expressed in embryonic/larval hemocyte (Drosophila) and 12 other tissues.
DR Genevisible; Q9VRN2; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0042594; P:response to starvation; ISS:UniProtKB.
DR CDD; cd00251; Mth_Ecto; 1.
DR Gene3D; 2.170.180.11; -; 1.
DR Gene3D; 2.30.160.11; -; 1.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR044860; Methusela_ecto_dom_1.
DR InterPro; IPR023311; Methusela_ecto_dom_2.
DR InterPro; IPR010596; Methuselah_N_dom.
DR InterPro; IPR036272; Methuselah_N_sf.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF06652; Methuselah_N; 1.
DR SUPFAM; SSF63877; SSF63877; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..518
FT /note="Probable G-protein coupled receptor Mth-like 2"
FT /id="PRO_0000013024"
FT TOPO_DOM 27..220
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..250
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..279
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..321
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..371
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..426
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 427..447
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 448..455
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..518
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 125
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 201
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 31..85
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 87..92
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 96..190
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 97..108
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 152..211
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT CONFLICT 85
FT /note="C -> G (in Ref. 3; AAM11297)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 518 AA; 60350 MW; 587A0E64EEE5D003 CRC64;
MIASSKMLLS ASILIYFLLN LQSSSAEIAD CSFYDTVDIS EGQRLSNGSY LYEGLLIPAH
LTAKYEFKLL ANGDKEQVPS HVRGCVCKLR TCVRFCCPHD HIMDMGECYA NMTTEENELL
DPMLNVTLDD GSVVQRHYKK ELMVQWDLPK PCDDMFYLDN RDIMDEYTLF ENGRLLRHYD
QVYLDKSEYC LQHRTFGEGN NNSIRIIPHN CLILPSRTGQ TVVMITSLIC LVLTIAVYLC
VKKLMNLEGK CFICYMMCLF FGYLFLLLDL WELSLDFCKA AGFLGYFFVM AAFFWLSIIS
RHYWKCLTNP CASMNIRSER AFLLYSCFAW AMPLALTGVT YLADNVVNNE EWQPRVGDEG
HCWIYTKSWS AMVYFYGPMV LLILFNITMF VLTAKHIIDS KRTLRKIARN EGRIQKLNSD
KQNYTQFLLL FTVMGMSWSF EIFSYLVQRE KLWVNIFLVA DYFNWSQGVI IFVLFILRRK
TLVLFKKQIF PKQRAFSRSA TQSTIESISQ TKRHFNMT
