MT4B_ARATH
ID MT4B_ARATH Reviewed; 85 AA.
AC Q42377; Q9SLN4;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Metallothionein-like protein 4B;
DE Short=MT-4B;
DE AltName: Full=EC protein homolog 2;
GN Name=MT4B; OrderedLocusNames=At2g23240; ORFNames=T20D16.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-85.
RC STRAIN=cv. Columbia; TISSUE=Dry seed;
RX PubMed=8580968; DOI=10.1046/j.1365-313x.1996.09010101.x;
RA Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J.,
RA Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D.,
RA Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P.,
RA Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.;
RT "Further progress towards a catalogue of all Arabidopsis genes: analysis of
RT a set of 5000 non-redundant ESTs.";
RL Plant J. 9:101-124(1996).
RN [4]
RP FUNCTION.
RX PubMed=18287486; DOI=10.1104/pp.108.115782;
RA Guo W.J., Meetam M., Goldsbrough P.B.;
RT "Examining the specific contributions of individual Arabidopsis
RT metallothioneins to copper distribution and metal tolerance.";
RL Plant Physiol. 146:1697-1706(2008).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND INDUCTION.
RX PubMed=22014117; DOI=10.1111/j.1365-3040.2011.02450.x;
RA Ren Y., Liu Y., Chen H., Li G., Zhang X., Zhao J.;
RT "Type 4 metallothionein genes are involved in regulating Zn ion
RT accumulation in late embryo and in controlling early seedling growth in
RT Arabidopsis.";
RL Plant Cell Environ. 35:770-789(2012).
CC -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC that bind various heavy metals (Probable). Functions as metal chelator
CC of copper (Cu) and zinc (Zn) (PubMed:18287486). Plays a role in storing
CC and distributing Zn ion in seed (PubMed:22014117).
CC {ECO:0000269|PubMed:18287486, ECO:0000269|PubMed:22014117,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22014117}. Nucleus
CC {ECO:0000269|PubMed:22014117}. Cell membrane
CC {ECO:0000269|PubMed:22014117}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q42377-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed specifically in seeds.
CC {ECO:0000269|PubMed:22014117}.
CC -!- DEVELOPMENTAL STAGE: During embryo development, expressed from torpedo
CC stage (6 days after pollination) to mature embryo.
CC {ECO:0000269|PubMed:22014117}.
CC -!- INDUCTION: By abscisic acid (ABA) and jasmonic acid. Down-regulated by
CC gibberellin. {ECO:0000269|PubMed:22014117}.
CC -!- MISCELLANEOUS: Plants silencing both MT4A and MT4B have reduced seed
CC weight and reduced seedling growth after germination.
CC {ECO:0000269|PubMed:22014117}.
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15 family.
CC {ECO:0000305}.
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DR EMBL; AC002391; AAB87107.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07432.1; -; Genomic_DNA.
DR EMBL; Z27049; CAA81575.1; -; mRNA.
DR EMBL; Z29896; CAA82835.1; -; mRNA.
DR PIR; T00508; T00508.
DR RefSeq; NP_179905.1; NM_127888.2. [Q42377-1]
DR AlphaFoldDB; Q42377; -.
DR SMR; Q42377; -.
DR STRING; 3702.AT2G23240.1; -.
DR PaxDb; Q42377; -.
DR PRIDE; Q42377; -.
DR EnsemblPlants; AT2G23240.1; AT2G23240.1; AT2G23240. [Q42377-1]
DR GeneID; 816856; -.
DR Gramene; AT2G23240.1; AT2G23240.1; AT2G23240. [Q42377-1]
DR KEGG; ath:AT2G23240; -.
DR Araport; AT2G23240; -.
DR TAIR; locus:2058593; AT2G23240.
DR eggNOG; ENOG502S74E; Eukaryota.
DR HOGENOM; CLU_166374_0_0_1; -.
DR InParanoid; Q42377; -.
DR OMA; CGEHCEC; -.
DR PhylomeDB; Q42377; -.
DR PRO; PR:Q42377; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q42377; baseline and differential.
DR Genevisible; Q42377; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0016020; C:membrane; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006829; P:zinc ion transport; IMP:TAIR.
DR InterPro; IPR000316; Metallthion_15.
DR Pfam; PF02068; Metallothio_PEC; 1.
DR PRINTS; PR00877; MTPLANTPEC.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Cytoplasm; Membrane; Metal-binding;
KW Metal-thiolate cluster; Nucleus; Reference proteome; Zinc.
FT CHAIN 1..85
FT /note="Metallothionein-like protein 4B"
FT /id="PRO_0000197424"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 85 AA; 8437 MW; 3B4065F1CD427912 CRC64;
MADTGKGSAS ASCNDRCGCP SPCPGGESCR CKMMSEASGG DQEHNTCPCG EHCGCNPCNC
PKTQTQTSAK GCTCGEGCTC ATCAA
