MOG2A_XENLA
ID MOG2A_XENLA Reviewed; 335 AA.
AC Q2KHS5;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=2-acylglycerol O-acyltransferase 2-A;
DE EC=2.3.1.22;
DE AltName: Full=Acyl-CoA:monoacylglycerol acyltransferase 2-A;
DE Short=MGAT2-A;
DE AltName: Full=Monoacylglycerol O-acyltransferase 2-A;
GN Name=mogat2-a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of diacylglycerol from 2-
CC monoacylglycerol and fatty acyl-CoA. {ECO:0000250|UniProtKB:Q3SYC2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2-acylglycerol + an acyl-CoA = a 1,2-diacyl-sn-glycerol +
CC CoA; Xref=Rhea:RHEA:32947, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:58342; EC=2.3.1.22;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-
CC (9Z-octadecenoyl)-sn-glycerol + CoA; Xref=Rhea:RHEA:37911,
CC ChEBI:CHEBI:52333, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:73990; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37912;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-
CC (9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:37915,
CC ChEBI:CHEBI:52323, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75342; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37916;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + butanoyl-CoA = 1-butyryl-2-
CC (9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38051,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57371, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75443; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38052;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + octanoyl-CoA = 1-octanoyl-2-
CC (9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38059,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57386, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75462; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38060;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + dodecanoyl-CoA = 1-dodecanoyl-
CC 2-(9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38063,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57375, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75463; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38064;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + tetradecanoyl-CoA = 1-
CC tetradecanoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38067, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75465;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38068;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-
CC hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38071, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75466;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38072;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + octadecanoyl-CoA = 1-
CC octadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38075, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75468;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38076;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + eicosanoyl-CoA = 1-eicosanoyl-
CC 2-(9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38079,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57380, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75470; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38080;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 2-(9Z-octadecenoyl)-glycerol =
CC 1-(9Z,12Z)-octadecadienoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38083, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75471;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38084;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 2-(9Z-octadecenoyl)-
CC glycerol = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-2-(9Z)-octadecenoyl-sn-
CC glycerol + CoA; Xref=Rhea:RHEA:38087, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57368, ChEBI:CHEBI:73990, ChEBI:CHEBI:75472;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38088;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-dodecanoylglycerol = 1-dodecanoyl-2-
CC (9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38115,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:75539,
CC ChEBI:CHEBI:75579; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38116;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-tetradecanoylglycerol = 1-
CC tetradecanoyl-2-(9Z-octadecenoyl)-glycerol + CoA;
CC Xref=Rhea:RHEA:38119, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75562, ChEBI:CHEBI:75582;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38120;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoylglycerol = 1-
CC hexadecanoyl-2-(9Z-octadecenoyl)glycerol + CoA; Xref=Rhea:RHEA:38123,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:69081,
CC ChEBI:CHEBI:75585; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38124;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-octadecanoylglycerol = 1-
CC octadecanoyl-2-(9Z)-octadecenoylglycerol + CoA; Xref=Rhea:RHEA:38127,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:75555,
CC ChEBI:CHEBI:75590; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38128;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z-octadecadienoyl)-glycerol =
CC 1-(9Z,12Z-octadecadienoyl)-2-(9Z-octadecenoyl)-glycerol + CoA;
CC Xref=Rhea:RHEA:38131, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75568, ChEBI:CHEBI:75614;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38132;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z,15Z-octadecatrienoyl)-
CC glycerol = 1-(9Z,12Z,15Z-octadecatrienoyl)-2-(9Z-octadecenoyl)-
CC glycerol + CoA; Xref=Rhea:RHEA:38135, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:75609, ChEBI:CHEBI:75610;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38136;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-
CC glycerol = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-(9Z-octadecenoyl)-
CC glycerol + CoA; Xref=Rhea:RHEA:38139, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:75611, ChEBI:CHEBI:75612;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38140;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl
CC hexadecanoate + CoA; Xref=Rhea:RHEA:38171, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57379, ChEBI:CHEBI:75586, ChEBI:CHEBI:75587;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38172;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-decanoylglycerol = 1-decanoyl-2-(9Z-
CC octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38019,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:75547,
CC ChEBI:CHEBI:85787; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38020;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-
CC (9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoA;
CC Xref=Rhea:RHEA:55340, ChEBI:CHEBI:34116, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:138734;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55341;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol =
CC 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38219,
CC ChEBI:CHEBI:52333, ChEBI:CHEBI:53753, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387; Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38220;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-mono-(9Z-
CC octadecenoyl)-glycerol = 1-O-(9Z-octadecenyl)-2,3-di-(9Z-
CC octadecenoyl)glycerol + CoA; Xref=Rhea:RHEA:55344, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:138734, ChEBI:CHEBI:138735;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55345;
CC Evidence={ECO:0000250|UniProtKB:Q3SYC2};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q3SYC2}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q3SYC2}. Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q3SYC2}.
CC -!- SIMILARITY: Belongs to the diacylglycerol acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; BC112915; AAI12916.1; -; mRNA.
DR RefSeq; NP_001089961.1; NM_001096492.1.
DR AlphaFoldDB; Q2KHS5; -.
DR DNASU; 735031; -.
DR GeneID; 735031; -.
DR KEGG; xla:735031; -.
DR CTD; 735031; -.
DR Xenbase; XB-GENE-5764987; mogat2.2.L.
DR OMA; PFYRDYI; -.
DR OrthoDB; 1347007at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000186698; Chromosome 2L.
DR Bgee; 735031; Expressed in intestine and 7 other tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:1990578; C:perinuclear endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0003846; F:2-acylglycerol O-acyltransferase activity; ISS:UniProtKB.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006640; P:monoacylglycerol biosynthetic process; ISS:UniProtKB.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR007130; DAGAT.
DR Pfam; PF03982; DAGAT; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Cytoplasm; Endoplasmic reticulum; Glycerol metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..335
FT /note="2-acylglycerol O-acyltransferase 2-A"
FT /id="PRO_0000249064"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 335 AA; 38254 MW; 1AB6A3F2D2EB23A8 CRC64;
MKIQFAPHNV PFERRLQTAA VLQWVFSFLA LAQTCILLFF VLLFTRFWII SVVYGVWWFL
DWDTPSKGGR RGEWLRRHVI WTYMKDYFPI TLVKTADLDP QQNYVVGSHP HGVLVAGAFT
NFCTEATGFH RLFPGITPYL LMLPLWFRAP FFRDYIMSGG LIPSDKDSAS YLLKNKAGGN
AVVIAVGGAP ESLDARPGAF TLLIKNRKGF VRLAILHGAS LVPVFSFGEN ELFDQVDNPR
GSWLRKIQEK LQKMMGVALP LFHARGVFQY SFGLIPYRKP IATIVGKPIR VEENPNPSSE
EVDKLHKIYM EELSKLFEEH KTKYNVPADK HLTFV
