MMPLA_MYCTU
ID MMPLA_MYCTU Reviewed; 1002 AA.
AC P9WJU1; L0T8N6; O50439; P65372;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Acyltrehalose exporter MmpL10 {ECO:0000305};
GN Name=mmpL10; OrderedLocusNames=Rv1183; ORFNames=MTV005.19;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=H37Rv;
RX PubMed=25124040; DOI=10.1074/jbc.m114.581199;
RA Belardinelli J.M., Larrouy-Maumus G., Jones V., Sorio de Carvalho L.P.,
RA McNeil M.R., Jackson M.;
RT "Biosynthesis and translocation of unsulfated acyltrehaloses in
RT Mycobacterium tuberculosis.";
RL J. Biol. Chem. 289:27952-27965(2014).
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=25331437; DOI=10.1128/jb.02015-14;
RA Touchette M.H., Holsclaw C.M., Previti M.L., Solomon V.C., Leary J.A.,
RA Bertozzi C.R., Seeliger J.C.;
RT "The rv1184c locus encodes Chp2, an acyltransferase in Mycobacterium
RT tuberculosis polyacyltrehalose lipid biosynthesis.";
RL J. Bacteriol. 197:201-210(2015).
CC -!- FUNCTION: Required for the biosynthesis of polyacyltrehalose (PAT) and
CC the transport of diacyltrehalose (DAT) and possibly PAT to the cell
CC surface. {ECO:0000269|PubMed:25124040}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:25124040}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant accumulates important amounts of
CC DAT and is devoid of PAT. DAT only accumulates inside the cells and is
CC not exported to the cell surface. {ECO:0000269|PubMed:25124040,
CC ECO:0000269|PubMed:25331437}.
CC -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC (TC 2.A.6) family. MmpL subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL123456; CCP43939.1; -; Genomic_DNA.
DR PIR; G70876; G70876.
DR RefSeq; NP_215699.1; NC_000962.3.
DR RefSeq; WP_003898761.1; NZ_NVQJ01000025.1.
DR AlphaFoldDB; P9WJU1; -.
DR STRING; 83332.Rv1183; -.
DR TCDB; 2.A.6.5.13; the resistance-nodulation-cell division (rnd) superfamily.
DR PaxDb; P9WJU1; -.
DR GeneID; 886068; -.
DR KEGG; mtu:Rv1183; -.
DR TubercuList; Rv1183; -.
DR eggNOG; COG2409; Bacteria.
DR OMA; FMAVLGK; -.
DR PhylomeDB; P9WJU1; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR InterPro; IPR004869; MMPL_dom.
DR InterPro; IPR004707; MmpL_fam.
DR Pfam; PF03176; MMPL; 2.
DR TIGRFAMs; TIGR00833; actII; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Lipid transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1002
FT /note="Acyltrehalose exporter MmpL10"
FT /id="PRO_0000103574"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 806..826
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 835..855
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 862..882
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 901..921
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 923..943
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1002 AA; 106415 MW; 5B49B70AF2076AD8 CRC64;
MVGCWVALAL VLPMAVPSLA EMAQRHPVAV LPADAPSSVA VRQMAEAFHE SGSENILVVL
LTDEKGLGAA DENVYHTLVD RLRNDAKDVV MLQDFLTTPP LREVLGSKDG KAWILPIGLA
GDLGTPKSYH AYTDVERIVK RTVAGTTLTA NVTGPAATVA DLTDAGARDR ASIELAIAVM
LLVILMVIYR NPVTMLLPLV TIGASLMTAQ ALVAGVSLVG GLAVSNQAIV LLSAMIAGAG
TDYAVFLISR YHEYVRLGEH PERAVQRAMM SVGKVIAASA ATVGITFLGM RFAKLGVFST
VGPALAIGIA VSFLAAVTLL PAILVLASPR GWVAPRGERM ATFWRRAGTR IVRRPKAYLG
ASLIGLVALA SCASLAHFNY DDRKQLPPSD PSSVGYAAME HHFSVNQTIP EYLIIHSAHD
LRTPRGLADL EQLAQRVSQI PGVAMVRGVT RPNGETLEQA RATYQAGQVG NRLGGASRMI
DERTGDLNRL ASGANLLADN LGDVRGQVSR AVAGVRSLVD ALAYIQNQFG GNKTFNEIDN
AARLVSNIHA LGDALQVNFD GIANSFDWLD SVVAALDTSP VCDSNPMCGN ARVQFHKLQT
ARDNGTLDKV VGLARQLQST RSPQTVSAVV NDLGRSLNSV VRSLKSLGLD NPDAARARLI
SMQNGANDLA SAGRQVADGV QMLVDQTKNM GIGLNQASAF LMAMGNDASQ PSMAGFNVPP
QVLKSEEFKK VAQAFISPDG HTVRYFIQTD LNPFSTAAMD QVNTIIDTAK GAQPNTSLAD
ASISMSGYPV MLRDIRDYYE RDMRLIVAVT VVVVILILMA LLRAIVAPLY LVGSVVISYM
SAIGLGVVVF QVFLGQELHW SVPGLAFVVL VAVGADYNML LASRLRDESA LGVRSSVIRT
VRCTGGVITA AGLIFAASMS GLLFSSIGTV VQGGFIIGVG ILIDTFVVRT ITVPAMATLL
GRASWWPGHP WQRCAPEEGQ MSARMSARTK TVFQAVADGS KR
