MLIC_ECOLI
ID MLIC_ECOLI Reviewed; 109 AA.
AC P28224;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Membrane-bound lysozyme inhibitor of C-type lysozyme {ECO:0000303|PubMed:18369469};
DE Flags: Precursor;
GN Name=mliC {ECO:0000303|PubMed:18369469}; Synonyms=ydhA;
GN OrderedLocusNames=b1639, JW1631;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-109.
RC STRAIN=K12;
RX PubMed=1356963; DOI=10.1128/jb.174.19.6033-6045.1992;
RA Lam H.-M., Winkler M.E.;
RT "Characterization of the complex pdxH-tyrS operon of Escherichia coli K-12
RT and pleiotropic phenotypes caused by pdxH insertion mutations.";
RL J. Bacteriol. 174:6033-6045(1992).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18369469; DOI=10.1371/journal.ppat.1000019;
RA Callewaert L., Aertsen A., Deckers D., Vanoirbeek K.G., Vanderkelen L.,
RA Van Herreweghe J.M., Masschalck B., Nakimbugwe D., Robben J.,
RA Michiels C.W.;
RT "A new family of lysozyme inhibitors contributing to lysozyme tolerance in
RT Gram-negative bacteria.";
RL PLoS Pathog. 4:E1000019-E1000019(2008).
RN [6]
RP STRUCTURE BY NMR OF 28-109, SUBUNIT, AND DISULFIDE BOND.
RX PubMed=16937244; DOI=10.1007/s10858-006-9032-y;
RA Revington M., Semesi A., Yee A., Arrowsmith C.H., Shaw G.S.;
RT "The solution structure of the protein ydhA from Escherichia coli.";
RL J. Biomol. NMR 35:295-300(2006).
CC -!- FUNCTION: Specifically inhibits C-type lysozymes.
CC {ECO:0000269|PubMed:18369469}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:16937244}.
CC -!- INTERACTION:
CC P28224; P0ACG8: hslR; NbExp=3; IntAct=EBI-562851, EBI-562824;
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000269|PubMed:18369469}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:18369469}. Note=Anchored to the
CC periplasmic side. {ECO:0000269|PubMed:18369469}.
CC -!- SIMILARITY: Belongs to the MliC family. Type 1 subfamily.
CC {ECO:0000305}.
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DR EMBL; U00096; AAC74711.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15400.2; -; Genomic_DNA.
DR EMBL; M92351; AAA24708.1; -; Genomic_DNA.
DR PIR; A43261; A43261.
DR RefSeq; NP_416156.4; NC_000913.3.
DR RefSeq; WP_000178044.1; NZ_STEB01000003.1.
DR PDB; 2F09; NMR; -; A=28-109.
DR PDBsum; 2F09; -.
DR AlphaFoldDB; P28224; -.
DR SMR; P28224; -.
DR BioGRID; 4263489; 136.
DR BioGRID; 851152; 1.
DR DIP; DIP-11726N; -.
DR IntAct; P28224; 5.
DR STRING; 511145.b1639; -.
DR jPOST; P28224; -.
DR PaxDb; P28224; -.
DR PRIDE; P28224; -.
DR EnsemblBacteria; AAC74711; AAC74711; b1639.
DR EnsemblBacteria; BAA15400; BAA15400; BAA15400.
DR GeneID; 946811; -.
DR KEGG; ecj:JW1631; -.
DR KEGG; eco:b1639; -.
DR PATRIC; fig|511145.12.peg.1710; -.
DR EchoBASE; EB1451; -.
DR eggNOG; COG3895; Bacteria.
DR HOGENOM; CLU_166586_0_0_6; -.
DR InParanoid; P28224; -.
DR OMA; EYRCDEK; -.
DR PhylomeDB; P28224; -.
DR BioCyc; EcoCyc:EG11488-MON; -.
DR EvolutionaryTrace; P28224; -.
DR PRO; PR:P28224; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR GO; GO:0060241; F:lysozyme inhibitor activity; IDA:EcoCyc.
DR Gene3D; 2.40.128.200; -; 1.
DR InterPro; IPR018660; MliC.
DR InterPro; IPR036328; MliC_sf.
DR Pfam; PF09864; MliC; 1.
DR SUPFAM; SSF141488; SSF141488; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Disulfide bond; Lipoprotein; Membrane;
KW Palmitate; Reference proteome; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 18..109
FT /note="Membrane-bound lysozyme inhibitor of C-type
FT lysozyme"
FT /id="PRO_0000168974"
FT SITE 71
FT /note="Directly involved in lysozyme active site
FT inhibition"
FT /evidence="ECO:0000250|UniProtKB:Q9I574"
FT SITE 85
FT /note="Directly involved in lysozyme active site
FT inhibition"
FT /evidence="ECO:0000250|UniProtKB:Q9I574"
FT LIPID 18
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 18
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT DISULFID 37..102
FT /evidence="ECO:0000269|PubMed:16937244"
FT STRAND 38..40
FT /evidence="ECO:0007829|PDB:2F09"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:2F09"
FT STRAND 50..66
FT /evidence="ECO:0007829|PDB:2F09"
FT STRAND 70..76
FT /evidence="ECO:0007829|PDB:2F09"
FT STRAND 81..84
FT /evidence="ECO:0007829|PDB:2F09"
FT STRAND 86..92
FT /evidence="ECO:0007829|PDB:2F09"
FT STRAND 97..100
FT /evidence="ECO:0007829|PDB:2F09"
SQ SEQUENCE 109 AA; 12568 MW; FB6E5F97F6893224 CRC64;
MTMKKLLIII LPVLLSGCSA FNQLVERMQT DTLEYQCDEK PLTVKLNNPR QEVSFVYDNQ
LLHLKQGISA SGARYTDGIY VFWSKGDEAT VYKRDRIVLN NCQLQNPQR
