MLH2_YEAST
ID MLH2_YEAST Reviewed; 695 AA.
AC Q07980; D6VY37;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=DNA mismatch repair protein MLH2;
DE AltName: Full=MutL protein homolog 2;
GN Name=MLH2; OrderedLocusNames=YLR035C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND INTERACTION WITH MLH1.
RX PubMed=10570173; DOI=10.1073/pnas.96.24.13914;
RA Wang T.-F., Kleckner N., Hunter N.;
RT "Functional specificity of MutL homologs in yeast: evidence for three Mlh1-
RT based heterocomplexes with distinct roles during meiosis in recombination
RT and mismatch correction.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:13914-13919(1999).
RN [4]
RP FUNCTION.
RX PubMed=10679328; DOI=10.1016/s0960-9822(00)00314-6;
RA Harfe B.D., Minesinger B.K., Jinks-Robertson S.;
RT "Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the
RT removal of frameshift intermediates in budding yeast.";
RL Curr. Biol. 10:145-148(2000).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION.
RX PubMed=15467363; DOI=10.1159/000080596;
RA Abdullah M.F.F., Hoffmann E.R., Cotton V.E., Borts R.H.;
RT "A role for the MutL homologue MLH2 in controlling heteroduplex formation
RT and in regulating between two different crossover pathways in budding
RT yeast.";
RL Cytogenet. Genome Res. 107:180-190(2004).
CC -!- FUNCTION: Involved in DNA mismatch repair (MMR), correcting insertion-
CC deletion loops (IDLs) resulting from DNA replication, DNA damage or
CC from recombination events between non-identical sequences during
CC meiosis. Component of a MutL heterodimer with MLH1, which probably
CC forms a ternary complex with the MutSbeta heterodimer that initially
CC recognizes the DNA mismatches. This complex is thought to be
CC responsible for directing the downsteam MMR events, including strand
CC discrimination, excision, and resynthesis. Plays a role in the repair
CC of heteroduplex sites present in meiotic recombination intermediates
CC and is involved in maintaining the genetic stability of simple sequence
CC repeats by correction of frameshift intermediates.
CC {ECO:0000269|PubMed:10570173, ECO:0000269|PubMed:10679328,
CC ECO:0000269|PubMed:15467363}.
CC -!- SUBUNIT: Heterodimer of MLH1 and MLH2.
CC -!- INTERACTION:
CC Q07980; P38920: MLH1; NbExp=5; IntAct=EBI-33369, EBI-11003;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 72 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutL/HexB family.
CC {ECO:0000305}.
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DR EMBL; Z73207; CAA97559.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09353.1; -; Genomic_DNA.
DR PIR; S64862; S64862.
DR RefSeq; NP_013135.1; NM_001181922.1.
DR AlphaFoldDB; Q07980; -.
DR SMR; Q07980; -.
DR BioGRID; 31309; 87.
DR ComplexPortal; CPX-1667; MutLbeta endonuclease complex.
DR DIP; DIP-2413N; -.
DR IntAct; Q07980; 7.
DR MINT; Q07980; -.
DR STRING; 4932.YLR035C; -.
DR iPTMnet; Q07980; -.
DR MaxQB; Q07980; -.
DR PaxDb; Q07980; -.
DR PRIDE; Q07980; -.
DR EnsemblFungi; YLR035C_mRNA; YLR035C; YLR035C.
DR GeneID; 850722; -.
DR KEGG; sce:YLR035C; -.
DR SGD; S000004025; MLH2.
DR VEuPathDB; FungiDB:YLR035C; -.
DR eggNOG; KOG1978; Eukaryota.
DR HOGENOM; CLU_024335_0_0_1; -.
DR InParanoid; Q07980; -.
DR OMA; ISNPFMI; -.
DR BioCyc; YEAST:G3O-32194-MON; -.
DR PRO; PR:Q07980; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q07980; protein.
DR GO; GO:0032300; C:mismatch repair complex; IBA:GO_Central.
DR GO; GO:0032390; C:MutLbeta complex; IPI:SGD.
DR GO; GO:0005634; C:nucleus; IPI:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0000710; P:meiotic mismatch repair; IC:ComplexPortal.
DR GO; GO:0006298; P:mismatch repair; IMP:SGD.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR014762; DNA_mismatch_repair_CS.
DR InterPro; IPR002099; DNA_mismatch_repair_N.
DR InterPro; IPR013507; DNA_mismatch_S5_2-like.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR038973; MutL/Mlh/Pms.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR PANTHER; PTHR10073; PTHR10073; 2.
DR Pfam; PF01119; DNA_mis_repair; 1.
DR SMART; SM01340; DNA_mis_repair; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR TIGRFAMs; TIGR00585; mutl; 1.
DR PROSITE; PS00058; DNA_MISMATCH_REPAIR_1; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Nucleus; Reference proteome.
FT CHAIN 1..695
FT /note="DNA mismatch repair protein MLH2"
FT /id="PRO_0000245569"
SQ SEQUENCE 695 AA; 78248 MW; DA5DA6AC86603A81 CRC64;
MTIHQLSPES QWKIVSSSFI YGPVAAVREL LDNSIDSGAK KVFIDVDSTT GGCEYISVKD
DGSGVDIIDR PSMCLEYTTS KMSSLGDISI LTTLGFRGEA LFLLSNLCNQ KGSMQVETKT
ADDVIGEKWL VDSKGGITNG KRYKVSCPVG TTVILRKLLG GLRARYLEIS SRPRKTFDEL
IYLINHYSLI HRNIRFYFSL VSLQKNGAIE RKQMQETLDP KISRARSLSL LARLKKPVPL
NFIVEENFVI DEKINLDLIL PRMVPESDVI NIKRRFKFLS VNERALSLNL ETGKTISKLL
SSIYRDFSLL DPMVWFINLN CDTKLLDVNI EPEKNDVMIK SFEVIFKKIE NKLKLLLEER
IGIETNMLGD KHVQPSINEK TSPALVIPTP DAENEISKGC GAVSGKDKTD IPQKNSDLIV
PTFYDEANLE NTTIVAATPS PTKFSEDKAL DEQTQLTISS YRSSSSGSMA SEDSTNWRHN
FQQELSENSE VAGAGSSTLP SSLTYNYIET IPENEDLELS KDASISNPFM ITKIRNVNKK
LSENLLEAKR TSCGDANKRG MPNERQLSNT EKKELIKLQQ TYGKRNNTVD MTIPRNSKKK
VTDNYIKKAS CMHKTRPKLM HFSEYTNNYV YTLKNEKIVK HDSDNFAKET LWLRSRDDAT
SPSSSLLQAL RAHVKKPGHI EATTNEWCLF TPDSP
