ID   MLE_CUTCT               Reviewed;         374 AA.
AC   P46057;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Muconate cycloisomerase 1;
DE            EC=5.5.1.1 {ECO:0000250|UniProtKB:P08310};
DE   AltName: Full=Cis,cis-muconate lactonizing enzyme I;
DE            Short=MLE;
DE   AltName: Full=Muconate cycloisomerase I;
OS   Cutaneotrichosporon cutaneum (Yeast) (Trichosporon cutaneum).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Trichosporonales; Trichosporonaceae; Cutaneotrichosporon.
OX   NCBI_TaxID=5554;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-42; 254-265 AND
RP   363-372.
RC   STRAIN=ATCC 58094;
RX   PubMed=8110801; DOI=10.1021/bi00173a045;
RA   Mazur P., Pieken W.A., Budihas S.R., Williams S.E., Wong S., Kozarich J.W.;
RT   "Cis,cis-muconate lactonizing enzyme from Trichosporon cutaneum: evidence
RT   for a novel class of cycloisomerases in eucaryotes.";
RL   Biochemistry 33:1961-1970(1994).
CC   -!- FUNCTION: Catalyzes a syn cycloisomerization.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-muconolactone = cis,cis-muconate + H(+);
CC         Xref=Rhea:RHEA:30031, ChEBI:CHEBI:15378, ChEBI:CHEBI:32379,
CC         ChEBI:CHEBI:58736; EC=5.5.1.1;
CC         Evidence={ECO:0000250|UniProtKB:P08310};
CC   -!- PATHWAY: Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-
CC       4,5-dihydro-2-furylacetate from catechol: step 2/3.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the cycloisomerase 2 family. {ECO:0000305}.
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DR   EMBL; L26042; AAA21782.1; -; mRNA.
DR   AlphaFoldDB; P46057; -.
DR   SMR; P46057; -.
DR   UniPathway; UPA00157; UER00259.
DR   GO; GO:0018849; F:muconate cycloisomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042952; P:beta-ketoadipate pathway; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR019405; Lactonase_7-beta_prop.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   Pfam; PF10282; Lactonase; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Isomerase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8110801"
FT   CHAIN           2..374
FT                   /note="Muconate cycloisomerase 1"
FT                   /id="PRO_0000171126"
SQ   SEQUENCE   374 AA;  41263 MW;  64E7B08D8A4D9196 CRC64;
     MAVAPTSYDI LMGTFRSPYL YTLTFDVLAR KLQVREVNEA TGGHNWLDVS PDGNTLYATV
     WGEPPKLTSY DIVRGGEYAT TKLSRNVASQ YMSGYVCSNN KAMYSACGPQ VDTFLVDDNG
     TLLDQPAVQS FNLLQGQEKN KANGTLDFGG LRHGGHSADL SPDGTKLYVA DIGRNCVWMY
     HVDRETGLLT EASKNIATRP HDGPRHAWPH PNGRIVYSLQ EHSSYVDAFR LTDDNKLEFL
     EGGCIIPDEK DHDKYWADEV RLSPMADVVF GSTRGLEEGT PGFVTAWNLR PDGTFASTEA
     THRFQTKTSG GWANAIAVCP NLGPNGEVFM TLTDSEVGFI QILAYTSDKG FEVVDELKIS
     TEKEHIMPAT TVWL