MHPD_AZOVD
ID MHPD_AZOVD Reviewed; 264 AA.
AC C1DRI8;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
DE EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
GN Name=mhpD {ECO:0000255|HAMAP-Rule:MF_01655}; OrderedLocusNames=Avin_15110;
OS Azotobacter vinelandii (strain DJ / ATCC BAA-1303).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Azotobacter.
OX NCBI_TaxID=322710;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DJ / ATCC BAA-1303;
RX PubMed=19429624; DOI=10.1128/jb.00504-09;
RA Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G.,
RA Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L.,
RA Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A.,
RA Houmiel K., Imperial J., Kennedy C., Larson T.J., Latreille P., Ligon L.S.,
RA Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I.,
RA Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L.,
RA Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H.,
RA Dean D.R., Dixon R., Wood D.;
RT "Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized
RT to support diverse anaerobic metabolic processes.";
RL J. Bacteriol. 191:4534-4545(2009).
CC -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC {ECO:0000255|HAMAP-Rule:MF_01655}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000255|HAMAP-Rule:MF_01655}.
CC -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
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DR EMBL; CP001157; ACO77726.1; -; Genomic_DNA.
DR RefSeq; WP_012700142.1; NC_012560.1.
DR AlphaFoldDB; C1DRI8; -.
DR SMR; C1DRI8; -.
DR STRING; 322710.Avin_15110; -.
DR EnsemblBacteria; ACO77726; ACO77726; Avin_15110.
DR KEGG; avn:Avin_15110; -.
DR eggNOG; COG3971; Bacteria.
DR HOGENOM; CLU_060136_4_1_6; -.
DR OMA; IRDWSIG; -.
DR OrthoDB; 1314120at2; -.
DR UniPathway; UPA00714; -.
DR Proteomes; UP000002424; Chromosome.
DR GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.90.850.10; -; 1.
DR HAMAP; MF_01655; MhpD; 1.
DR InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR InterPro; IPR023793; Keto_pentenoate-hydratase.
DR Pfam; PF01557; FAA_hydrolase; 1.
DR SUPFAM; SSF56529; SSF56529; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; Lyase.
FT CHAIN 1..264
FT /note="2-keto-4-pentenoate hydratase"
FT /id="PRO_1000215853"
SQ SEQUENCE 264 AA; 27699 MW; 27FE0F33ADE25E46 CRC64;
MSVTQQTLEQ LAAALRGAEA NGTAIEPLRG AIGEDNGTAA YAIQRLNVAH AVAAGRRVVG
RKIGLTNPKV QRQLGVDQPD FGTLFADMSH GDNEVLSIKR VLQPKIEAEI ALILERDLPR
ADTTFAELAE ATGWVLPALE IVGSRVRDWN IRFVDTVADN ASSGCFVLGG PVRRLAGVDL
RRAAMRMTRN GEEVSSGSGA ECLGHPLNAA VWLARTMAGL GEPLKAGDLI LTGALGPMVG
VAAGDRFEAE IEGIGQVGVE FGAA
