ID   MHPD3_PSEPU             Reviewed;         269 AA.
AC   Q706S1;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=2-keto-4-pentenoate hydratase 3 {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase 3 {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD3 {ECO:0000255|HAMAP-Rule:MF_01655};
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Strain RR21;
RX   PubMed=9791097; DOI=10.1128/jb.180.21.5505-5514.1998;
RA   Ravatn R., Studer S., Zehnder A.J., van der Meer J.R.;
RT   "Int-B13, an unusual site-specific recombinase of the bacteriophage P4
RT   integrase family, is responsible for chromosomal insertion of the 105-
RT   kilobase clc element of Pseudomonas sp. Strain B13.";
RL   J. Bacteriol. 180:5505-5514(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Strain RR21;
RX   PubMed=12823813; DOI=10.1046/j.1365-2958.2003.03548.x;
RA   Sentchilo V., Zehnder A.J., van der Meer J.R.;
RT   "Characterization of two alternative promoters and a transcription
RT   regulator for integrase expression in the clc catabolic genomic island of
RT   Pseudomonas sp. strain B13.";
RL   Mol. Microbiol. 49:93-104(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Strain RR21;
RX   PubMed=16484212; DOI=10.1128/jb.188.5.1999-2013.2006;
RA   Gaillard M., Vallaeys T., Vorholter F.J., Minoia M., Werlen C.,
RA   Sentchilo V., Puhler A., van der Meer J.R.;
RT   "The clc element of Pseudomonas sp. strain B13, a genomic island with
RT   various catabolic properties.";
RL   J. Bacteriol. 188:1999-2013(2006).
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
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DR   EMBL; AJ617740; CAE92879.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q706S1; -.
DR   SMR; Q706S1; -.
DR   PRIDE; Q706S1; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase.
FT   CHAIN           1..269
FT                   /note="2-keto-4-pentenoate hydratase 3"
FT                   /id="PRO_0000337799"
SQ   SEQUENCE   269 AA;  28759 MW;  9A9B4B8A3B95CFF0 CRC64;
     MTPQQREEAA QSLYQAMQSG KPIAPLRDTF PDMNVDDAYA IQSINTQRRI SLGRRVVGRK
     IGLTSVVVQQ QLGVDEPDFG ALFDDMSFGD AETIPLSILH QPKVEAEIGF VLGRDLDTEQ
     PTHQEVLQAV DYVVPALEIV GSRIADWNIK FVDTVADNAS SGVYVLGSTP ISPRGLDLSL
     VGMCLSRRGE PVSTGAGAAC LGTPLNAVVW LARTMSRLGK PLRAGELILS GALGPMVAVK
     PGDVFECHIN GVGSVRTEFE SNQMNGVAA