ID   MHBT_KLEOX              Reviewed;         452 AA.
AC   Q5EXK5;
DT   20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=3-hydroxybenzoate transporter MhbT {ECO:0000303|PubMed:22729544};
GN   Name=mhbT {ECO:0000312|EMBL:AAW63412.1};
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M5a1;
RX   PubMed=15782938; DOI=10.1016/j.micres.2004.09.003;
RA   Liu D.Q., Liu H., Gao X.L., Leak D.J., Zhou N.Y.;
RT   "Arg169 is essential for catalytic activity of 3-hydroxybenzoate 6-
RT   hydroxylase from Klebsiella pneumoniae M5a1.";
RL   Microbiol. Res. 160:53-59(2005).
RN   [2]
RP   FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION,
RP   AND MUTAGENESIS OF ASP-82; VAL-311 AND ASP-314.
RC   STRAIN=M5a1;
RX   PubMed=22729544; DOI=10.1128/aem.01511-12;
RA   Xu Y., Gao X., Wang S.H., Liu H., Williams P.A., Zhou N.Y.;
RT   "MhbT is a specific transporter for 3-hydroxybenzoate uptake by Gram-
RT   negative bacteria.";
RL   Appl. Environ. Microbiol. 78:6113-6120(2012).
CC   -!- FUNCTION: Uptake of 3-hydroxybenzoate (3HBA).
CC       {ECO:0000269|PubMed:22729544}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=5.57 uM for 3-hydroxybenzoate {ECO:0000269|PubMed:22729544};
CC         Vmax=18.35 nmol/min/mg enzyme {ECO:0000269|PubMed:22729544};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:22729544}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: Induced by 3-hydroxybenzoate. {ECO:0000269|PubMed:22729544}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Aromatic
CC       acid:H(+) symporter (AAHS) (TC 2.A.1.15) family. {ECO:0000305}.
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DR   EMBL; AY648560; AAW63412.1; -; Genomic_DNA.
DR   RefSeq; WP_004870412.1; NZ_QPKC01000003.1.
DR   AlphaFoldDB; Q5EXK5; -.
DR   SMR; Q5EXK5; -.
DR   STRING; 571.MC52_27535; -.
DR   GeneID; 64336515; -.
DR   eggNOG; COG2271; Bacteria.
DR   OrthoDB; 1121832at2; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..452
FT                   /note="3-hydroxybenzoate transporter MhbT"
FT                   /id="PRO_0000435145"
FT   TRANSMEM        22..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        112..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        253..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        290..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        319..338
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        385..407
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        412..434
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         82
FT                   /note="D->A: Loss of activity."
FT                   /evidence="ECO:0000269|PubMed:22729544"
FT   MUTAGEN         311
FT                   /note="V->W: Loss of activity."
FT                   /evidence="ECO:0000269|PubMed:22729544"
FT   MUTAGEN         314
FT                   /note="D->A: Loss of activity."
FT                   /evidence="ECO:0000269|PubMed:22729544"
SQ   SEQUENCE   452 AA;  48068 MW;  B5D2B6A535E3A5FE CRC64;
     MTQRLELQKL LNAAPVGARQ WRVIICCFLV VMLDGFDTAA IGFIAPDIRS HWQLTAGDLA
     PLFGAGLLGL TAGALLCGPL SDRFGRKRVI EFCVALFGLF SLLSAFAPDL QMLVFLRFLT
     GLGLGGAMPN TITMTSEYLP ARRRGALVTL MFCGFTLGSA FGGVVSAQLV PLIGWHGILV
     LGGVLPLILA VALVPLLPES PRWQIRRQLP QATIARTVGA ITGERYNDTH FWLDEPAAGA
     KGSISQLFAG RQLAITLMLW VVFFMSLLII YLLSSWMPTL LNHRGIDLQH ASWVTAAFQI
     GGTFGALLLG VLMDRFNPYR VLALSYGLGA VCIVMIGLSE NGLWLMALAI FGTGIGISGS
     QVGLNALTAT LYPTQSRATG VSWSNAVGRC GAIVGSLSGG VMMAMNFSFD TLFFVIAVPA
     VVSAVMLILL TLAVRNPTSV PAALPSAGIV NK