MET7_NEUCR
ID MET7_NEUCR Reviewed; 621 AA.
AC P38675; Q7RV94;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 3.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Cystathionine gamma-synthase;
DE EC=2.5.1.48;
DE AltName: Full=O-succinylhomoserine (thiol)-lyase;
GN Name=met-7; ORFNames=NCU02430;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-621.
RX PubMed=1531800; DOI=10.1016/0378-1119(92)90698-o;
RA Crawford J.M., Geever R.F., Asch D.K., Case M.E.;
RT "Sequence and characterization of the met-7 gene of Neurospora crassa.";
RL Gene 111:265-266(1992).
CC -!- FUNCTION: Catalyzes the formation of L-cystathionine from O-succinyl-L-
CC homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In
CC the absence of thiol, catalyzes gamma-elimination to form 2-
CC oxobutanoate, succinate and ammonia (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-cysteine + O-succinyl-L-homoserine = H(+) + L,L-
CC cystathionine + succinate; Xref=Rhea:RHEA:20397, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:35235, ChEBI:CHEBI:57661,
CC ChEBI:CHEBI:58161; EC=2.5.1.48;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
CC -!- SUBUNIT: Both met-3 and met-7 are required to form a functional
CC cystathionine gamma-synthase.
CC -!- SIMILARITY: Belongs to the trans-sulfuration enzymes family. MET7
CC subfamily. {ECO:0000305}.
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DR EMBL; CM002242; EAA30199.2; -; Genomic_DNA.
DR EMBL; M64066; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; JQ1524; JQ1524.
DR RefSeq; XP_959435.2; XM_954342.3.
DR AlphaFoldDB; P38675; -.
DR SMR; P38675; -.
DR STRING; 5141.EFNCRP00000003145; -.
DR EnsemblFungi; EAA30199; EAA30199; NCU02430.
DR GeneID; 3875582; -.
DR KEGG; ncr:NCU02430; -.
DR VEuPathDB; FungiDB:NCU02430; -.
DR HOGENOM; CLU_011302_1_0_1; -.
DR InParanoid; P38675; -.
DR OMA; FPVAKQY; -.
DR UniPathway; UPA00051; UER00077.
DR Proteomes; UP000001805; Chromosome 7, Linkage Group VII.
DR GO; GO:0003962; F:cystathionine gamma-synthase activity; IBA:GO_Central.
DR GO; GO:0102028; F:cystathionine gamma-synthase activity (acts on O-phosphohomoserine); IEA:UniProtKB-EC.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0019346; P:transsulfuration; IBA:GO_Central.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR InterPro; IPR000277; Cys/Met-Metab_PyrdxlP-dep_enz.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR Pfam; PF01053; Cys_Met_Meta_PP; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Methionine biosynthesis; Pyridoxal phosphate;
KW Reference proteome; Transferase.
FT CHAIN 1..621
FT /note="Cystathionine gamma-synthase"
FT /id="PRO_0000114779"
FT MOD_RES 429
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000255"
FT CONFLICT 194
FT /note="R -> A (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 621 AA; 68938 MW; 7A3B6166218B162A CRC64;
MPPFELGDPI PSETAHAVSV SLPTWSANVG YEEGQDWVVK RMATGYPRFF IHRTIQAFAA
DIVATHVSTK AKRTSDITAM LFPTPTIASR CVDFIRSRAP ADVCSNIEVV NLVLDMSDPE
ARALEPLCPS ISAVIMPQDG FPFAKQYWQH SGDGVSSRRA EFCHGLFKDG LLRPDTELRN
AAVSAAKPCR GPKRYQRQAS LDAGGNQQTI MHGHIHRATG ETAMIQETSR FLEERFGRNL
DLSFVHPAKS AIKRRIAGAL RSADHDLGGS PSLSEKQMSS NTRGIANLRE EDIYLFPCGM
NAIFHAHRAL YSIRTPPGST PLKSVNFGFP YVDTLKILEK FNPSGALFYG HGSKSDLDDL
ETRLESGERY LALFCEFPGN PLLTCPDLVR IRELADKYEF AVVVDETIGT FANVNVLQFA
DIVVSSLTKI FSGDCNVMGG GAIFNPNSRY YSALKSFVQQ QLEDTYWPED VIFMERNSRD
FVARIDRVNA NAEAICRVLQ DHPLVKTLYY PKYNDSRANY EAVKLPQGGY GGLISVVLKG
KKQAVAFYDA IETAKGPSLG TNFTLTSPYV LLAHYQELDW AEQYGVDRNL IRISVGLEGT
DELINVFTRA LKVAEEQSQY P
